Journal
ENVIRONMENTAL MICROBIOLOGY
Volume 10, Issue 8, Pages 2179-2183Publisher
WILEY
DOI: 10.1111/j.1462-2920.2008.01634.x
Keywords
-
Categories
Ask authors/readers for more resources
Streptococcus uberis is an environmental bovine mastitis pathogen capable of UV-inducible SOS mutagenesis. Bacterial SOS systems can be induced by several chemicals including also antibiotics used in clinical practice. Here, we have studied the effect of ciprofloxacin, a fluoroquinolone antibiotic and known inducer of SOS, on mutations leading to antibiotic resistance in S. uberis. Mutation frequencies and spectra were compared in a wild-type S. uberis strain and its Delta umuC derivative. The results revealed that concentrations of ciprofloxacin corresponding to 0.3-0.5x minimum inhibitory concentration (MIC) induce mutagenesis independent of UmuC. Partial sequencing of the rpoB gene of individual rifampin-resistant clones from wild-type and Delta umuC strains revealed a similar but complex pattern of point mutations including transitions, transversions and deletions/insertions. It was previously shown that UV induces mainly transition-type mutations and UmuC is essential for the process. Thus, the results presented here demonstrate that S. uberis employs distinct mechanisms for ciprofloxacin and UV-induced mutagenesis, which is a striking difference to Escherichia coli SOS model.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available