Journal
ENVIRONMENTAL GEOCHEMISTRY AND HEALTH
Volume 35, Issue 4, Pages 535-549Publisher
SPRINGER
DOI: 10.1007/s10653-013-9513-3
Keywords
Chlorinated aliphatic hydrocarbons; Degradation; Microbiota; 16S rRNA; Remediation
Categories
Funding
- Fundamental Research Funds for the Central Universities [2010ZY05]
- National Program of Control and Treatment of Water Pollution [2009ZX07424-002]
- National Natural Science Foundation [40972162]
Ask authors/readers for more resources
Pollution of groundwater with chlorinated aliphatic hydrocarbons (CAHs) is a serious environmental problem which is threatening human health. Microorganisms are the major participants in degrading these contaminants. Here, groundwater contaminated for a decade with CAHs was investigated. Numerical simulation and field measurements were used to track and forecast the migration and transformation of the pollutants. The diversity, abundance, and possible activity of groundwater microbial communities at CAH-polluted sites were characterized by molecular approaches. The number of microorganisms was between 5.65E+05 and 1.49E+08 16S rRNA gene clone numbers per liter according to quantitative real-time PCR analysis. In 16S rRNA gene clone libraries constructed from samples along the groundwater flow, eight phyla were detected, and Proteobacteria were dominant (72.8 %). The microbial communities varied with the composition and concentration of pollutants. Meanwhile, toluene monooxygenases and methane monooxygenases capable of degradation of PCE and TCE were detected, demonstrating the major mechanism for PCE and TCE degradation and possibility for in situ remediation by addition of oxygen in this study.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available