Journal
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
Volume 104, Issue -, Pages 16-25Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envexpbot.2014.03.003
Keywords
Abiotic stress; Glycine betaine; N-methyltransferase; Transgenic rice
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Funding
- National Basic Research Program of China (973 Program) [2011CB100401]
- Key Project from Chongqing Local Government [2010AA1019]
- National Science Fund of China for Distinguished Young Scientists [30825030]
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Glycine betaine (GB) is an osmoprotectant that protects plants from abiotic stress. GB is biosynthesized by choline dehydrogenation/oxidation and glycine methylation. The latter pathway involves glycine as a substrate and produces GB via successive methylation of glycine by two different N-methyltransferase enzymes; glycine sarcosine methyltransferase (GSMT), and sarcosine dimethylglycine methyltransferase (SDMT). In this study, Agrobacterium-mediated gene transformation was used to produce transgenic rice (Oryza sativa L., cv. Nipponbare) plants containing ApGSMT and ApDMT genes isolated from Aphanothece halophytica. The co-expression of both ApGSMT and ApDMT transgenes resulted in a significant increase of GB biosynthesis and enhanced tolerance to salt and cold stresses in the transgenic rice plants. These results demonstrate the potential of bioengineering for glycine N-methyltransferase genes in crop plants tolerance to abiotic stress. (C) 2014 Elsevier B.V. All rights reserved.
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