Article
Engineering, Environmental
Junhong Guo, Shuxin Li, Marian Brestic, Na Li, Peng Zhang, Lei Liu, Xiangnan Li
Summary: This study investigates the effects of the co-existence of nanomaterials on plant growth. The results show that the co-existence of nanoplastics and zinc oxide nanoparticles increases the concentration of H2O2 and ABA, and affects protein phosphorylation, photosynthesis, carbon fixation, nitrogen metabolism, and arginine and proline metabolisms. Furthermore, the co-existence of nanomaterials causes damage to the systems of antioxidant and carbohydrate metabolisms in plants.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Engineering, Environmental
Shuxin Li, Tianya Wang, Junhong Guo, Yuefan Dong, Zongshuai Wang, Lei Gong, Xiangnan Li
Summary: This study found that microplastics negatively affect the growth of barley plants by limiting root development and altering enzyme activities in roots. There were differences in carbohydrate and ROS metabolism enzyme activities between leaves and roots under microplastics treatment, as well as distinct changes in phytohormone concentrations. The findings suggest that the phytohormone regulatory network plays a key role in regulating enzyme activities in response to microplastics in barley plants.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Fan Ye, Miao Jiang, Peng Zhang, Lei Liu, Shengqun Liu, Chunsheng Zhao, Xiangnan Li
Summary: Exogenous melatonin can enhance the drought tolerance of crops under drought conditions by regulating the rhizosphere microbial community and improving nutrient conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Miao Jiang, Fan Ye, Fulai Liu, Marian Brestic, Xiangnan Li
Summary: The application of rhizospheric melatonin can enhance the tolerance of barley to low temperature by regulating photosynthetic carbon assimilation and redox homeostasis. This effect is potentially related to the changes in microbial diversity in the rhizosphere.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Zengke Ma, Juncheng Wang, Chengdao Li, Panrong Ren, Lirong Yao, Baochun Li, Yaxiong Meng, Xiaole Ma, Erjing Si, Ke Yang, Xunwu Shang, Huajun Wang
Summary: This study reveals the phosphorylation proteins in barley roots under P starvation and resupply conditions, and their functions in various metabolic pathways. The phosphorylated proteins show significant changes under P starvation and resupply conditions, mainly participating in carbohydrate metabolism, phytohormones, signal transduction, and other metabolic processes.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Juncheng Wang, Zengke Ma, Chengdao Li, Panrong Ren, Lirong Yao, Baochun Li, Yaxiong Meng, Xiaole Ma, Erjing Si, Ke Yang, Xunwu Shang, Huajun Wang
Summary: Barley, as a major cereal crop, with low Pi demand, is advantageous for studying phosphorus deficiency tolerance mechanisms. The study revealed that barley roots can regulate specific changes in succinylated protein sites in response to Pi stress and specific metabolic processes. Understanding the metabolic pathways of succinylated protein regulation characteristics will improve phosphate acquisition and utilization efficiency in crops.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Agronomy
Giorgia Capasso, Giorgia Santini, Mariagioia Petraretti, Sergio Esposito, Simone Landi
Summary: Barley, a major crop globally, has been genetically improved for higher yields but weakened in resilience to adverse environments. Climate change demands crop varieties that can grow under stress conditions, where wild varieties and landraces may play a crucial role.
Article
Agronomy
Karel Klem, Jan Kren, Jan Simor, Daniel Kovac, Petr Holub, Petr Misa, Ilona Svobodova, Vojtech Lukas, Petr Lukes, Hana Findurova, Otmar Urban
Summary: Sensitive methods for estimating N status in malting barley during the vegetation period are crucial to ensure optimal yield and grain quality. Utilizing N nutrition index, N uptake, and artificial neural network based on multiple spectral reflectance wavelengths can significantly improve the accuracy of N status estimation, reducing the impact of various factors such as genotype, sowing density, and year fluctuations.
Article
Biochemistry & Molecular Biology
Junhong Guo, Gerrit T. S. Beemster, Fulai Liu, Zongming Wang, Xiangnan Li
Summary: ABA deficiency leads to decreased low temperature tolerance in barley. Proteomic analysis revealed changes in photosynthesis and metabolism processes. Additionally, ABA deficiency caused damage to chloroplasts, decreased Fv/Fm, and altered enzyme activities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ibrahim I. Shabbaj, Hamada AbdElgawad, Mansour A. Balkhyour, Abdurazag Tammar, Mahmoud M. Y. Madany
Summary: This study investigated the toxicity of In2O3-NPs in C3 and C4 plants and the mitigating effects of eCO(2). It found that In2O3-NPs significantly reduced growth and photosynthesis, induced oxidative damage, and impaired nutrient homeostasis in plants. However, eCO(2) alleviated these effects and improved plant growth and nutrient uptake. The study also highlighted the role of SOD in mitigating the hazardous effects of In2O3-NPs.
Article
Agronomy
Roxana Savin, C. Mariano Cossani, Rachid Dahan, Jamal Y. Ayad, Rossella Albrizio, Mladen Todorovic, Mohammed Karrou, Gustavo A. Slafer
Summary: Water management is crucial for improving grain yield in the dryland systems of the Mediterranean basin. This study explored the potential of nitrogen (N) fertilisation to increase grain yield by improving water use efficiency. The results showed that durum wheat and barley responded positively to N fertilisation under rainfed cultivation, leading to an average increase of 26% in grain yield compared to unfertilised controls. The response to N fertilisation was observed in both low and high yield potential cases, with grain number per unit area and total biomass at maturity being the main factors influencing grain yield. Barley and durum wheat had similar responses to N fertilisation.
EUROPEAN JOURNAL OF AGRONOMY
(2022)
Article
Plant Sciences
Xiaxia Tian, Qiang Yao, Zedong Zhang, Xiangrui Cheng, Jianfeng Qin, Zhensheng Kang, Jie Zhao
Summary: This study reports the first occurrence of crown rust on barley in China, and reveals different levels of host resistance to the pathogen among different plant species.
Article
Plant Sciences
Maja Cohen, Kendra Hertweck, Maxim Itkin, Sergey Malitsky, Bareket Dassa, Andreas M. Fischer, Robert Fluhr
Summary: This study characterized leaf senescence in two near-isogenic barley lines differing in the HvNAM1 allele, highlighting the role of metabolites and genes associated with proteostasis, lipid metabolism, and nitrogen remobilization. The results showed that thylakoid lipids were converted to neutral lipids during senescence, accompanied by an accumulation of sugars and a decrease in nitrogen compounds. RNA-Seq analysis suggested lipid catabolism and carbon skeleton production as a replacement for diminished carbon supply from photosynthesis. Differences in gene expression between the two lines indicated a potential role of heat stress transcription factors and chaperone-encoding genes in leaf longevity control.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Runhong Gao, Guimei Guo, Hongwei Xu, Zhiwei Chen, Yingbo Li, Ruiju Lu, Chenghong Liu, Jianmin Chen
Summary: The study found that under low nitrogen stress, the high nitrogen use efficiency mutant A9-29 exhibited better growth and nitrogen uptake compared to the wild-type barley cultivar Hua30, with larger root area and higher expression levels of nitrogen assimilation genes.
PLANT GROWTH REGULATION
(2021)
Article
Engineering, Environmental
Zongshuai Wang, Shuxin Li, Shulian Jian, Fan Ye, Tianya Wang, Lei Gong, Xiangnan Li
Summary: Increasing plastic consumption results in the accumulation of polystyrene nanoplastics (PS-NPs) in agricultural soil, negatively affecting plant growth and the stress tolerance of crops.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Applied
Eliana Dell'Olmo, Rosa Gaglione, Katia Pane, Sergio Sorbo, Adriana Basile, Sergio Esposito, Angela Arciello
Summary: The study demonstrated the efficacy of Acca sellowiana fruit acetonic extract on both human MDR cancer cells and bacterial biofilm, including resistant tumor and bacterial strains. These findings intriguingly expand the panel of activities of the fruit extract, opening up interesting perspectives for therapeutic applications.
NATURAL PRODUCT RESEARCH
(2021)
Article
Plant Sciences
Giovanna Salbitani, Sabrina Cipolletta, Vincenza Vona, Catello Di Martino, Simona Carfagna
Summary: Galdieria phlegrea is capable of autotrophy, heterotrophy, and mixotrophy, with the ability to switch metabolic pathways in different conditions, allowing for rapid growth recovery and photosynthetic functionality.
JOURNAL OF PLANT GROWTH REGULATION
(2021)
Review
Green & Sustainable Science & Technology
Giovanna Salbitani, Simona Carfagna
Summary: Ammonium is a convenient nitrogen source for plant cell metabolism, but can be toxic to microalgae at high concentrations, depending on species. Its high concentrations in the environment pose risks like water pollution and eutrophication, necessitating nutrient removal from wastewater before discharge, with microalgae offering a sustainable alternative solution.
Review
Plant Sciences
Daniela Castiglia, Simone Landi, Sergio Esposito
Summary: Algal species harbor untapped potential for the discovery of new compounds, particularly those from extreme environments with unique alleles and proteins suitable for a wide range of biotechnology applications. The review focuses on the latest applications of algae-derived proteins and the innovative use of modified algae as bio-reactors. The study also discusses the pharmaceutical applications, including the development of drugs to combat viruses like SARS-CoV-2, and the utilization of extremophiles as bio-factories for specific protein and molecule production.
Article
Plant Sciences
Valeria Paradisone, Eloy Navarro-Leon, Juan M. Ruiz, Sergio Esposito, Begona Blasco
Summary: This study investigated the effects of silicon on barley plants under zinc deficiency and toxicity conditions, demonstrating that CaSiO3 can alleviate the impact of zinc stress on barley plants and promote plant growth.
ACTA PHYSIOLOGIAE PLANTARUM
(2021)
Article
Plant Sciences
Simone Landi, Giorgia Capasso, Sergio Esposito
Summary: Low temperatures pose significant physiological stress on plants, but the involvement of the oxidative pentose phosphate pathway may play a crucial role in responding to cold stress.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Eloy Navarro-Leon, Valeria Paradisone, Francisco Javier Lopez-Moreno, Juan Jose Rios, Sergio Esposito, Begona Blasco
Summary: Salinity is an environmental factor that reduces plant productivity, but modifying Ca2+ cation exchangers (CAX) transporters could increase plant tolerance to salinity. Through Targeting Induced Local Lesions in Genomes (TILLING), researchers generated mutants of Brassica rapa CAX1a transporter, with BraA.cax1a-4 showing improved photosynthetic performance under saline conditions. This study highlights BraA.cax1a-4 as a useful mutation for enhancing photosynthesis in plants grown in saline conditions.
Review
Agronomy
Giorgia Capasso, Giorgia Santini, Mariagioia Petraretti, Sergio Esposito, Simone Landi
Summary: Barley, a major crop globally, has been genetically improved for higher yields but weakened in resilience to adverse environments. Climate change demands crop varieties that can grow under stress conditions, where wild varieties and landraces may play a crucial role.
Article
Agronomy
Sawsen Ayadi, Salma Jallouli, Zoubeir Chamekh, Ines Zouari, Simone Landi, Zied Hammami, Fatma Ezzahra Ben Azaiez, Mokhtar Baraket, Sergio Esposito, Youssef Trifa
Summary: Nitrogen is crucial for plant growth and the use of fertilizers can optimize agricultural environments. This study investigated the grain yield, grain protein content, and nitrogen use efficiency of different genotypes of wheat. The results suggest that nitrogen use efficiency and grain protein content are important traits for identifying wheat with higher nitrogen use efficiency.
Article
Biochemistry & Molecular Biology
Nunzia Esercizio, Mariamichela Lanzilli, Simone Landi, Lucio Caso, Zhaohui Xu, Genoveffa Nuzzo, Carmela Gallo, Emiliano Manzo, Sergio Esposito, Angelo Fontana, Giuliana D'Ippolito
Summary: Capnophilic lactic fermentation (CLF) is an anaplerotic pathway identified in Thermotoga bacteria that enables non-competitive synthesis of hydrogen and L-lactic acid. This study discovered and characterized CLF in Thermotoga sp. strain RQ7, opening up new possibilities for molecular investigation of the pathway. Key CLF enzymes are up-regulated in the CLF-performing strains, and the up-regulation of V-ATPase plays a crucial role in supporting the simultaneous synthesis of hydrogen and lactic acid.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Simone Landi, Giorgia Santini, Ermenegilda Vitale, Gabriella Di Natale, Giulia Maisto, Carmen Arena, Sergio Esposito
Summary: The Caulerpa species are of scientific interest due to their invasion in the Mediterranean ecological niche, production of valuable natural metabolites, and response to zinc contamination. Caulerpa racemosa showed efficient uptake of zinc from contaminated seawater.
Review
Microbiology
Carmen Laezza, Giovanna Salbitani, Simona Carfagna
Summary: The increasing interest in microalgae as sources of new biomolecules and environmental remediators has led to investigations and applications in various fields. However, the presence of fungal contaminants in microalgal cultures is a major problem that affects biomass production and quality.
Review
Biochemistry & Molecular Biology
Giovanna Salbitani, Viviana Maresca, Piergiorgio Cianciullo, Rosanna Bossa, Simona Carfagna, Adriana Basile
Summary: Heavy-metal pollution is a widely discussed problem. It has been shown that heavy metals have biological effects on both animals and plants, including oxidative stress and genotoxicity. Plants, especially metal-tolerant species, have developed various strategies to counteract exposure to toxic metal concentrations. This review discusses the role of non-protein thiol compounds and antioxidant molecules in bryophytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Simone Landi, Paola Punzo, Roberta Nurcato, Rossella Albrizio, Walter Sanseverino, Riccardo Aiese Cigliano, Pasquale Giorio, Florinda Fratianni, Giorgia Batelli, Sergio Esposito, Stefania Grillo
Summary: 'Corbarino' (COR) and 'Lucariello' (LUC) are Mediterranean long shelf-life tomato landraces that produce high-quality fruits under low water input cultivation. Understanding their unique drought stress tolerance at morpho-physiological and molecular levels may be crucial for their utilization in breeding programs.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Review
Plant Sciences
Giorgia Santini, Daniela Castiglia, Maryanna Martina Perrotta, Simone Landi, Giulia Maisto, Sergio Esposito
Summary: Plastic pollution is a growing environmental concern, with microplastics and nanoplastics having detrimental effects on plants, including photosynthetic injuries, oxidative stress, and protein damage.
Article
Plant Sciences
Jingqiu Xia, Jiawen Liang, Mengmeng Yu, Rui Wang, Chen Sun, Huishan Song, Qinghua Xu, Jing Cang, Yuying Wang, Da Zhang
Summary: The MED complex acts as a bridge to regulate transcription by connecting transcription factors and specific gene promoters. This study demonstrates the important role of MED25 in freezing tolerance in wheat, possibly through its interaction with the jasmonate signaling pathway and cold-responsive genes.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Asier R. Larrinaga, Luis Sampedro, Rafael Zas
Summary: This study investigated the allocation to growth, reproduction, and defence in maritime pine under different resource availability and simulated herbivory treatments. The results showed that fertilization increased tree growth and seed quantity but not seed quality. Needle clipping and methyl-jasmonate treatment increased needle resin and phenol concentrations. Overall, there were complex interactions among the life-history dimensions, suggesting that pairwise approaches are insufficient to unravel these complexities.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Shuang Han, Shuxian Li, Ya Li, Qingchen Zhang, Yuanyuan Xu, Wenjing Wang, Xiao Qin Zhu, Dongli Pei
Summary: The xanthophyll cycle plays a significant role in protecting plants from membrane peroxidation induced by intense light exposure. This study identified 240 differentially expressed genes associated with respiratory chain, lipid metabolism, antioxidant activity, and flavonoid metabolism. The overexpression of the CmBCH1 gene resulted in the upregulation of genes involved in scavenging reactive oxygen species and encoding antioxidants, leading to the accumulation of xanthophyll, zeaxanthin, chlorophylls, and anthocyanins. The study highlighted the potential of BCH1 in regulating the xanthophyll cycle and enhancing tolerance to intense light stress in transgenic plants.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Yuzhu Ding, Hejun Ren, Xinyu Hao, Ruonan Zhang, Jianjun Hao, Jinliang Liu, Hongyu Pan, Yan Wang
Summary: This study demonstrated that co-expressing two exogenous genes, tfdB and bphC, in Arabidopsis thaliana improved the tolerance and removal efficiency of PCB28. Transgenic plants also exhibited increased enzymatic activities under PCB28 stress. The co-expression of tfdB and bphC in A. thaliana resulted in nearly twofold increase in PCB28 removal rates from soil. This research suggests that co-expressing two genes holds great potential for enhancing phytoremediation efficiency and mitigating PCB-induced toxicity stress on plants.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Nosheen Kabir, Sumbal Wahid, Shoaib Ur Rehman, Ghulam Qanmber
Summary: Trichomes are specialized plant structures that protect plants and are regulated by complex gene networks and signaling pathways. Gene expression, cell cycle regulation, and differentiation determine whether cells become trichomes. Transcription factors, epigenetic modifications, and phytohormones play important roles in trichome development. The integration of phytohormonal and transcriptional networks contributes to the diversity and adaptability of trichomes in plants.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Enrique Mateos-Naranjo, Jesus Alberto Perez-Romero, Giacomo Puglielli, Javier Lopez-Jurado, Jennifer Mesa-Marin, Eloisa Pajuelo, Ignacio David Rodriguez-Llorente, Susana Redondo-Gomez
Summary: The impact of multifactorial abiotic stress combinations on plant functional responses remains controversial. The halophyte Salicornia ramosissima showed unique functional responses depending on the specific stress factors involved. Under extreme conditions, there was a more negative impact on plant functional traits, but the presence of beneficial microorganisms, especially in combination with elevated atmospheric CO2 concentration, mitigated these negative effects.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Mengjiao Ruan, Wenliang He, Rui He, Xiangxiang Wang, Jinxin Wei, Yujie Zhu, Ruiling Li, Zhijun Jiang, Xiaofan Na, Xiaomin Wang, Yurong Bi
Summary: The study reveals the important role of AOX2 in Arabidopsis seed germination by regulating ABA signal and ROS homeostasis under salt stress. Additionally, ABI3/ABI4 are essential for salt-induced AOX2 expression.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Ning Li, Peiyao Yu, Yanling Zeng, Jiali Chen, Wenhai Yang, Guannan Qin, Shenxiu Du, Xiao Han, Li-Jun Huang
Summary: In eukaryotic cells, epigenetic modifications of DNA and histones play a crucial role in gene expression regulation. ROXY19, a plant-specific CC-type glutaredoxin, was found to strongly repress a subset of genes regulated by class II TGA factors through its association with the TPL/TPR family. The ectopic expression of ROXY19 in Arabidopsis plants led to hypersensitivity to xenobiotic chemicals due to the silencing of detoxification pathway genes.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Yang Li, Chenyang Feng, Yijing Xing, Meng Li, Xiaoning Wang, Qingjie Du, Huaijuan Xiao, Juanqi Li, Jiqing Wang
Summary: In this study, researchers found that CsNLP1 gene in cucumber plays an important role in nitrogen utilization. The study also revealed the regulation mechanism of CsNLP1 in cucumber growth and nitrogen assimilation.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Lei Chen, Cheng Jiang, Li Ye, Yue Gao, Xilin Hou
Summary: This study identified and characterized 163 C2H2-type zinc finger proteins in non-heading Chinese cabbage. Two of these proteins, BcZAT12 and BcZAT10, were found to be closely related to cold tolerance and acted as transcriptional repressors in regulating cold resistance. Furthermore, BcZAT12 was shown to directly bind to the promoter of BcCBF1 and inhibit its activity. Additionally, interactions between BcZAT12 and other proteins such as BcABF2/4 were observed. These findings provide insights into the functional roles and regulatory mechanisms of C2H2-ZFPs in non-heading Chinese cabbage under cold stress.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Ommolbanin Tourajzadeh, Halimeh Piri, Amir Naserin, Mohammad mahdi Cahri
Summary: The combination of NB and reduced irrigation water depth can mitigate the negative effects of salinity and drought stresses on quinoa and improve its water productivity. The use of NB up to 2% alleviated salinity and drought stress and increased yield, while decreasing salinity and irrigation water depth increased water productivity. The appropriate use of NB can reduce the negative effects of stress and promote the sustainable production of quinoa.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Jing Li, Yujie Wu, Xiaohui Feng, Tabassum Hussain, Kai Guo, Xiaojing Liu
Summary: This study investigated the growth and physiological responses of cherry tomatoes under different salt conditions and found that nonuniform salinity conditions improved photosynthetic characteristics, water use efficiency, and fruit yield. These findings are important for improving tomato productivity on salt-affected lands.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Ruonan Li, Ruying Wang, Meng Li, Yunpu Zheng, Xiaxiang Zhang, Zhimin Yang, Jingjin Yu
Summary: The study found that elevated CO2 can increase stolon growth and carbohydrate accumulation in stolon nodes and internodes. Foliar application of auxin enhances this effect, while auxin transport inhibitor has no effect.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Jian-Jun Tao, Cui-Cui Yin, Yang Zhou, Yi-Hua Huang, Shou-Yi Chen, Jin-Song Zhang
Summary: Soil salinization is a major obstacle for agriculture sustainability, as it severely affects plant growth and crop yield. Ethylene, a stress hormone, plays a crucial role as a signal molecule in coordinating plant growth and stress response in the model plant Arabidopsis. However, the roles and mechanisms of ethylene in most crop plants under salinity remain undefined. This review summarizes the universal roles of ethylene and focuses on its divergent roles in rice and other crop species under salinity, particularly in terms of ethylene biosynthesis and signal transduction. The contradictions between ethylene production and signaling in salt response are also discussed.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)
Article
Plant Sciences
Chang Guo, Xiaona Wang, Qi Wang, Zipeng Zhao, Bing Xie, Lang Xu, Ruijie Zhang
Summary: This review provides an overview of the effects of ozone pollution on plant secondary metabolism. It explores the role of plant secondary metabolism in defense against ozone stress and adaptation to ozone-polluted environments, while summarizing the severity of ozone pollution.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2024)