Journal
ENVIRONMENT INTERNATIONAL
Volume 50, Issue -, Pages 56-65Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envint.2012.09.009
Keywords
Carbon nanoparticles; Multivariate analysis; Nanotoxicity; Spectroscopy; Synchrotron radiation
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Funding
- Natural Environment Research Council [ceh010010] Funding Source: researchfish
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1112433] Funding Source: National Science Foundation
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Our ability to identify the mechanisms by which carbon-based nanomaterials (CBNs) exert toxicity in cells is constrained by the lack of standardized methodologies to assay endpoint effects. Herein we describe a method of mechanistically identifying the effects of various CBN types in both prokaryotic and eukaryotic cells using multi-beam synchrotron radiation-based Fourier-transform infrared imaging (SR-FTIRI) at diffraction-limited resolution. This technique overcomes many of the inherent difficulties of assaying nanotoxicity and demonstrates exceptional sensitivity in identifying the effects of CBNs in cells at environmentally-relevant concentrations. We identify key mechanisms of nanotoxicity as the alteration of Amide and lipid biomolecules, but propose more specific bioactivity of CBNs occurs as a result of specific interactions between CBN structural conformation and cellular characteristics. (C) 2012 Elsevier Ltd. All rights reserved.
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