Article
Genetics & Heredity
Lingyu Jiang, Wenjing Hu, Yexiong Qian, Qiaoyu Ren, Jing Zhang
Summary: Heat shock factors (Hsfs) and heat shock proteins (Hsps) are crucial in plant development and defense against abiotic stress. This study identified 25 ZmHsf and 22 ZmHsp70 genes in maize, and analyzed their structural characteristics and phylogenetic relationships. Most of the ZmHsf and ZmHsp70 genes showed different expression patterns during maize development stages and responded to heat stress. Co-expression and protein-protein interaction network analyses provided insights into the regulatory network associated with these genes in maize. Subcellular location analysis revealed functional diversification in heat stress response for maize Hsp70 proteins.
Article
Multidisciplinary Sciences
Sharif Hasan Siddiqui, Mousumee Khan, Hosung Choe, Darae Kang, Kwanseob Shim
Summary: This study investigated the structure and functional expression of heat shock proteins (HSPs) in different chicken organs using meta-analysis. The results showed that the expression of HSPs varied among different organs and under different stress levels. Heat stress significantly changed the expression of HSP70 in the chicken liver. The findings highlight the diverse expression patterns of HSPs in chicken organs.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Qianqian Qin, Yujun Zhao, Jiajun Zhang, Li Chen, Weina Si, Haiyang Jiang
Summary: In this study, an HSF gene ZmHsf11 was cloned from maize and found to negatively regulate the expression of oxidative stress-related genes, increase ROS levels, and decrease proline content, thereby reducing plant tolerance to heat stress.
Article
Biochemistry & Molecular Biology
Digvijay Singh, Prajna Tripathi, Rahul Sharma, Sonam Grover, Janendra K. Batra
Summary: The N-terminal domain of Mtb ClpB plays a crucial role in the interaction with its co-partners and in the prevention of aggregation of proteins. The substrate binding pocket identified in this study, comprising of nine amino acid residues forming an alpha-helix, is important for the function of ClpB in repairing and resolubilizing aggregated proteins.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Plant Sciences
Sique Chen, Hongrui Cao, Baolin Huang, Xiujuan Zheng, Kangjing Liang, Guo-Liang Wang, Xinli Sun
Summary: This study reveals the functions of WRKY10 transcription factor and VQ8 protein in rice thermotolerance. WRKY10 negatively regulates thermotolerance by modulating the balance of reactive oxygen species (ROS) and hypersensitive response, while VQ8 functions antagonistically to positively regulate thermotolerance.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Urszula Korotko, Karolina Chwialkowska, Izabela Sanko-Sawczenko, Miroslaw Kwasniewski
Summary: The study found that under heat stress, the level of DNA methylation decreases in Arabidopsis thaliana, especially after returning to normal conditions. Analysis of gene ontology enrichment and regulatory pathways showed that genes with differential DNA methylation are mainly associated with stress responses, including heat stress. This indicates an active demethylation phenomenon rather than passive demethylation under the influence of heat stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Roshan Kumar Singh, Mehanathan Muthamilarasan, Manoj Prasad
Summary: In this study, a novel small heat shock protein, SisHSP21.9, in foxtail millet was identified and characterized for its role in conferring tolerance to high-temperature stress. Overexpression of SisHSP21.9 in rice improved the plant's survival and overall grain yield during high-temperature stress, indicating its potential for designing thermotolerant crops. Further research is needed as tolerance to abiotic stresses is polygenic.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biology
Huanhuan Qi, Xiaoke Chen, Sen Luo, Hongzeng Fan, Jinghua Guo, Xuehai Zhang, Yinggen Ke, Pingfang Yang, Feng Yu
Summary: This study analyzed the distribution, gene structure, and promoter elements of HSP20 in maize. The results showed that ZmHSP20s exhibited different expression patterns under heat stress and had binding relationships with certain heat stress transcription factors. These findings provide valuable insights for further understanding the function of HSP20 and improving maize's resistance to abiotic stresses.
Article
Plant Sciences
Wenxu Wang, Jiaojiao Zhang, Lijuan Ai, Dan Wu, Bing Li, Lingang Zhang, Liqun Zhao
Summary: This study demonstrates the relationship between CNGC6 and H2O2 in plant responses to heat shock, showing that CNGC6 may stimulate the accumulation of H2O2 in a manner dependent on an increase in cytosolic Ca2+. Additionally, CNGC6 enhances the gene transcription of HS factors and the accumulation of HS proteins via H2O2 to improve plant thermotolerance.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Vivek Kumar, Archana Chugh
Summary: In this study, a novel peptide-based macromolecule delivery strategy targeting chloroplasts was successfully developed, offering advantages such as non-invasiveness, biocompatibility, low-toxicity, and target-specific delivery.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kamilla Gomori, Melissa Herwig, Roua Hassoun, Heidi Budde, Nusratul Mostafi, Simin Delalat, Suvasini Modi, Merima Begovic, Tamara Szabados, Judit Pipis, Nikolett Farkas-Morvay, Istvan Lepran, Arpad Kovacs, Andreas Mugge, Peter Ferdinandy, Aniko Gorbe, Peter Bencsik, Nazha Hamdani
Summary: This study investigates the effects of volume overload on the heart using a rat model and finds that it leads to cardiac hypertrophy, mechanical dysfunction, dysregulated signaling pathways, and increased oxidative stress. Furthermore, oxidative stress causes impairment of the protein quality control system.
Review
Biochemistry & Molecular Biology
Vickram Ramkumar, Debashree Mukherjea, Asmita Dhukhwa, Leonard P. Rybak
Summary: Hearing loss can result from exogenous insults that generate oxidative stress and inflammation, with cisplatin ototoxicity being one major cause. The mechanisms associated with cisplatin-induced hearing loss, including pathways for cisplatin entry and toxic pathways activated by cisplatin, have been discussed. Protective G-protein coupled receptors and heat shock proteins in the cochlea may offer potential avenues for prevention and treatment.
Article
Plant Sciences
Elena Kreis, Justus Niemeyer, Marco Merz, David Scheuring, Michael Schroda
Summary: In plant cells, the CLP/HSP100 chaperone family member HSP101 plays a crucial role in resolving heat-induced protein aggregates. However, the role of the chloroplast family member CLPB3 in this process remains unclear. In this study, two clpb3 mutants in Chlamydomonas reinhardtii were characterized, revealing that chloroplast CLPB3 is required for resolving heat-induced protein aggregates and conferring thermotolerance. CLPB3 was found to interact with HSP22E/F near the thylakoid membranes, suggesting a possible role in disentangling protein aggregates from the thylakoid membrane system.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Parasitology
Nancy A. Aguoru, Ruth S. Kirk, Anthony J. Walker
Summary: This study provides a detailed molecular bioinformatic analysis of heat shock proteins (HSPs) in the human parasitic blood fluke Schistosoma mansoni. The results reveal the classification, expression patterns, protein interactions, and phosphorylation sites of HSPs in different stages of the parasite's life cycle. The findings suggest that HSPs play important roles in parasite survival and development, and offer insights for future investigations and therapeutics development against schistosomiasis.
PARASITES & VECTORS
(2022)
Article
Food Science & Technology
Simeng Chen, Jie Zhou, Chidimma Juliet Igbokwe, Yuqing Duan, Meihong Cai, Yuanqing He, Haihui Zhang
Summary: This study investigated the effect and potential mechanism of RDPEER, an antioxidant oligopeptide derived from watermelon seeds, on heat stress-induced liver injury and cell damage. The results showed that supplementation with RDPEER attenuated hepatocyte injury by increasing cell viability, reducing ROS production, improving antioxidant enzyme activity, and decreasing MDA content. RDPEER also inhibited HPA axis hyperactivity, transaminase activity, and inflammatory factor levels, while activating the endogenous antioxidant pathway and reducing inflammatory factor transcription and heat shock protein expression. Overall, RDPEER supplementation enhanced antioxidant capacity and alleviated liver damage caused by heat stress, suggesting its potential use in nutritional interventions.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Review
Agronomy
Liangjie Niu, Ruiqi Hao, Xiaolin Wu, Wei Wang
Article
Plant Sciences
Yibo Wang, Xiuyun Wang, Ruixue Yang, Liangjie Niu, Wei Wang
Article
Plant Sciences
Qinbin Zhang, Hui Liu, Xiaolin Wu, Wei Wang
Article
Plant Sciences
Tingting Wang, Zhenfeng Zang, Shuai Wang, Yuke Liu, Hezhong Wang, Wei Wang, Xiuli Hu, Jinhua Sun, Fuju Tai, Rui He
Summary: The study demonstrated that IFQA can promote plant seedlings' root growth, positively influence root active absorption and protein expression, and enhance plants' tolerance to osmotic stress.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Yanhua Li, Liangjie Niu, Xiaolin Wu, Claudia Faleri, Fuju Tai, Man Zhang, Hui Liu, Wei Wang, Giampiero Cai
Summary: The study identified 38 homologous genes of ZmCP03 in maize and analyzed the protein motifs, conserved domains, gene structures, and duplication patterns of 39 CPs, revealing their evolutionary relationship and potential functions. The results suggested that ZmCP03 was relatively conservative and stable during evolution. This study provides valuable insights into the role of ZmCP03 in pollen development and germination in maize.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Liangjie Niu, Cui Du, Wenrui Wang, Man Zhang, Wei Wang, Hui Liu, Jinghua Zhang, Xiaolin Wu
Summary: This study compared the development of ABA synthesis-deficient mutant (vp5) and normal maize (Vp5) embryos through ABA content and transcriptome analysis. The results showed that differential ABA synthesis affected seed maturation, and identified gene modules that were negatively correlated with ABA content and involved in metabolic and cellular processes. These findings are important for understanding the molecular mechanism of ABA signaling and developing high dehydration tolerance maize suitable for mechanical harvesting.
Article
Biochemistry & Molecular Biology
Jinghua Zhang, Qiaofeng Pai, Ling Yue, Xiaolin Wu, Hui Liu, Wei Wang
Summary: Cytokinins affect cell division in the female gametophyte by regulating the expression of cell cycle genes, thus impacting the alternating generations in angiosperms.
Review
Biochemistry & Molecular Biology
Hanwei Du, Jiajia Chen, Haiying Zhan, Shen Li, Yusheng Wang, Wei Wang, Xiuli Hu
Summary: The calcium ion (Ca2+) acts as an important second messenger in plants, playing a crucial role in growth, development, and stress adaptation. Calcium-dependent protein kinases (CDPKs), acting as central Ca2+ sensor proteins, are widely present in plants. In maize, the response processes of ZmCDPKs to abiotic stresses have been well studied, and it has been found that they are regulated by various abiotic stresses and transmit signals to downstream molecules through protein interaction or phosphorylation, leading to cascade reactions and involvement in hormone and reactive oxygen signaling regulation. ZmCDPKs play an indispensable role in regulating maize growth, development, and stress responses. This review summarizes the roles of ZmCDPKs in integrating different signaling pathways to regulate maize's response to abiotic stress, providing insights into the molecular mechanisms of ZmCDPKs in maize's tolerance to abiotic stress and creating new opportunities for agricultural applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Hui Liu, Songbo Song, Hui Zhang, Yanhua Li, Liangjie Niu, Jinghua Zhang, Wei Wang
Summary: Drought is a global threat that affects agricultural production. Plants have evolved several adaptive strategies to cope with drought, among which stomatal closure is a critical mechanism. Abscisic acid, reactive oxygen species, and calcium ion play pivotal roles in controlling stomatal closure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Environmental Sciences
Liangjie Niu, Chunyang Li, Wei Wang, Jinghua Zhang, Monica Scali, Weiqiang Li, Hui Liu, Fuju Tai, Xiuli Hu, Xiaolin Wu
Summary: In this review, proteomic studies on Cd-tolerance mechanisms in Cd-tolerant crops and Cdhyperaccumulating plants were summarized, especially focusing on the similarities and differences across plant species. Enhanced differentially abundant proteins identified through proteomic studies can be potential targets for developing Cd-hyperaccumulators and Cd-tolerant crops, which is crucial for ensuring food security for the growing global population. The article also discusses the methodological drawbacks in current proteomic studies and suggests the utilization of better protocols and advanced techniques to enhance the reliability and applicability of future Cd-stress-related studies in plants. This review provides insights into improving phytoremediation efficiency and studying the molecular mechanisms of Cd enrichment in plants.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Yanhua Li, Liangjie Niu, Xiaoli Zhou, Hui Liu, Fuju Tai, Wei Wang
Summary: A pollen-specific cysteine protease (PCP) plays an important role in pollen germination and drought response in maize. Overexpression of PCP inhibits pollen germination, while mutation of PCP promotes it to some extent. Additionally, overexpression of PCP enhances drought tolerance in maize plants with increased antioxidant enzyme activities and decreased root cortical cell numbers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Liangjie Niu, Liangwei Liu, Jinghua Zhang, Monica Scali, Wei Wang, Xiuli Hu, Xiaolin Wu
Summary: Maize is a significant source of starch, which is crucial for bioethanol production. However, the current production process involves high temperatures and additional equipment, leading to increased costs. This review discusses the features of starch granules suitable for efficient enzymatic digestion and explores the role of key proteins in starch metabolism. The authors propose modifying key enzymes through genetic engineering to synthesize easily digested starch granules in maize seeds, offering valuable insights for developing specialized maize cultivars for the bioethanol industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Hui Liu, Songbo Song, Mengyao Liu, Yangwei Mu, Ying Li, Yuxin Xuan, Liangjie Niu, Hui Zhang, Wei Wang
Summary: In this study, an NAC transcription factor ZmNAC20 was identified and found to regulate drought stress response in maize. Overexpression of ZmNAC20 improved drought resistance in maize by promoting stomatal closure and activating the expression of stress-responsible genes. These findings provide valuable insights and new clues for improving crop drought resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Benshuai Liang, Jiahui Cao, Ruilin Wang, Chenjie Fan, Wei Wang, Xiuli Hu, Rui He, Fuju Tai
Summary: In this study, the CIPK32 gene from Zea mays was isolated and characterized. It was found that CIPK32 plays a role in promoting seed germination under stress conditions by modulating GA signals and reducing ABA levels.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Hao Yang, Yulong Zhao, Ning Chen, Yanpei Liu, Shaoyu Yang, Hanwei Du, Wei Wang, Jianyu Wu, Fuju Tai, Feng Chen, Xiuli Hu
Summary: This study identified a novel AC protein, ZmRPP13-LK3, which catalyzes ATP to produce cAMP and plays a role in ABA-regulated heat resistance. The results demonstrate that ZmRPP13-LK3 interacts with ZmABC2 and is associated with the ABA signaling pathway.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)