Journal
ENERGIES
Volume 6, Issue 3, Pages 1794-1801Publisher
MDPI AG
DOI: 10.3390/en6031794
Keywords
bioethanol; carrot pomace; Kluyveromyces marxianus; simultaneous saccharification and fermentation
Categories
Funding
- National Science Council [NSC98-2221-E-036-029, NSC 100-2221-E-036-025]
- Ministry of Economic Affairs of R.O.C. [98-EC-17-A-13-S1-116]
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Carrot pomace, a major agricultural waste from the juice industry, was used as a feedstock for bioethanol production by fermentation with the thermotolerant yeast Kluyveromyces marxianus. Treatment of the carrot pomace with Accellerase (TM) 1000 and pectinase at 50 C for 84 h, resulted in conversion of 42% of its mass to fermentable sugars, mainly glucose, fructose, and sucrose. Simultaneous saccharification and fermentation (SSF) at 42 degrees C was performed on 10% (w/v) carrot pomace; the concentration of ethanol reached 18 g/L and the yield of ethanol from carrot pomace was 0.18 g/g. The highest ethanol concentration of 37 g/L was observed with an additional charge of 10% supplemented to the original 10% of carrot pomace after 12 h; the corresponding yield was 0.185 g/g. Our results clearly demonstrated the potential of combining a SSF process with thermotolerant yeast for the production of bioethanol using carrot pomace as a feedstock.
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