4.5 Article

The Physiological Role of Arcuate Kisspeptin Neurons in the Control of Reproductive Function in Female Rats

Journal

ENDOCRINOLOGY
Volume 155, Issue 3, Pages 1091-1098

Publisher

OXFORD UNIV PRESS INC
DOI: 10.1210/en.2013-1544

Keywords

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Funding

  1. Medical Research Council
  2. Biotechnology and Biological Sciences Research Council
  3. National Institute for Health Research
  4. Wellcome Trust an Integrative Mammalian Biology Capacity Building Award
  5. Society for Endocrinology
  6. National Institute for Health Research Career Development fellowship
  7. Wellcome Trust Research Training fellowship
  8. European Obesity Consortium studying the Hypothalamus and its Interaction with the Periphery Grant [HEALTH- 2009- 241592]
  9. Biotechnology and Biological Sciences Research Council [BB/I00842X/1, BB/E52708X/1, BB/J002232/1] Funding Source: researchfish
  10. Medical Research Council [G1000455] Funding Source: researchfish
  11. National Institute for Health Research [CDF-2009-02-05, CL-2011-21-006, NF-SI-0513-10080, NF-SI-0507-10337] Funding Source: researchfish
  12. BBSRC [BB/J002232/1, BB/I00842X/1, BB/E52708X/1] Funding Source: UKRI
  13. MRC [G1000455] Funding Source: UKRI

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Kisspeptin plays a pivotal role in pubertal onset and reproductive function. In rodents, kisspeptin perikarya are located in 2 major populations: the anteroventral periventricular nucleus and the hypothalamic arcuate nucleus (ARC). These nuclei are believed to play functionally distinct roles in the control of reproduction. The anteroventral periventricular nucleus population is thought to be critical in the generation of the LH surge. However, the physiological role played by the ARC kisspeptin neurons remains to be fully elucidated. We used bilateral stereotactic injection of recombinant adeno-associated virus encoding kisspeptin antisense into the ARC of adult female rats to investigate the physiological role of kisspeptin neurons in this nucleus. Female rats with kisspeptin knockdown in the ARC displayed a significantly reduced number of both regular and complete oestrous cycles and significantly longer cycles over the 100-day period of the study. Further, kisspeptin knockdown in the ARC resulted in a decrease in LH pulse frequency. These data suggest that maintenance of ARC-kisspeptin levels is essential for normal pulsatile LH release and oestrous cyclicity.

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