Article
Biology
Haruka Narita, Tomohiro Shima, Ryo Iizuka, Sotaro Uemura
Summary: The N-terminal region of Argonaute2 can form amyloid-like aggregates, which may regulate the RNA silencing activity of DmAgo2.
Article
Biochemistry & Molecular Biology
Liya G. Bobyleva, Sergey A. Shumeyko, Elmira I. Yakupova, Alexey K. Surin, Oxana V. Galzitskaya, Hiroshi Kihara, Alexander A. Timchenko, Maria A. Timchenko, Nikita V. Penkov, Alexey D. Nikulin, Mariya Yu. Suvorina, Nikolay V. Molochkov, Mikhail Yu. Lobanov, Roman S. Fadeev, Ivan M. Vikhlyantsev, Alexander G. Bobylev
Summary: The research investigated the in vitro aggregation and amyloid properties of sMyBP-C, showing rapid formation of large aggregates and the formation of sMyBP-C oligomers consisting of 7-10 monomers. The results suggest a high aggregability of sMyBP-C in vitro and indicate the unlikeliness of pathological amyloid formation in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Daniel Kachkin, Kirill V. Volkov, Julia Sopova, Alexander G. Bobylev, Sergei A. Fedotov, Sergei G. Inge-Vechtomov, Oxana Galzitskaya, Yury O. Chernoff, Aleksandr A. Rubel, Anna Y. Aksenova
Summary: This study assessed the amyloid properties of RAD51 protein in vitro and in a bacterial system. The purified human RAD51 protein formed amyloid-like aggregates with a cross-beta fibrillar structure, and were stained with amyloid-specific dyes. Cytoplasmic aggregates of RAD51 were also observed in cell cultures overexpressing RAD51.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ravit Malishev, Shani Ben-Zichri, Ofek Oren, Nitzan Shauloff, Tal Peretz, Ran Taube, Niv Papo, Raz Jelinek
Summary: The BIM-BH3 motif is a key pro-apoptotic domain that forms toxic amyloid fibrils, leading to cell death and impacting mitochondrial functions.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Oxana V. Galzitskaya, Sergei Y. Grishin, Anna V. Glyakina, Nikita V. Dovidchenko, Anastasiia V. Konstantinova, Sergey V. Kravchenko, Alexey K. Surin
Summary: In recent years, there has been an increase in the number of identified diseases associated with the accumulation of amyloid proteins, which are known to cause degenerative diseases in humans. Therefore, it is important to develop strategies for the search and development of effective inhibitors of amyloid formation. This review focuses on three amyloidogenic peptides and proteins - A beta, alpha-synuclein, and insulin - and analyzes existing and prospective strategies for the development of effective and non-toxic inhibitors of amyloid formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Diana C. Rodriguez Camargo, Sean Chia, Joseph Menzies, Benedetta Mannini, Georg Meisl, Martin Lundqvist, Christin Pohl, Katja Bernfur, Veronica Lattanzi, Johnny Habchi, Samuel Ia Cohen, Tuomas P. J. Knowles, Michele Vendruscolo, Sara Linse
Summary: The aggregation of human islet amyloid polypeptide (IAPP) is associated with type II diabetes, with IAPP oligomers identified as the most cytotoxic species. Secondary nucleation is the main mechanism for generating new aggregates, with toxicity peaking at the maximum secondary nucleation rate. Compounds targeting IAPP secondary nucleation may be the most effective therapeutic candidates for type II diabetes.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Giovanni Smaldone, Daniela Caruso, Annamaria Sandomenico, Emanuela Iaccarino, Annalia Foca, Alessia Ruggiero, Menotti Ruvo, Luigi Vitagliano
Summary: The three members of the GADD45 protein family are involved in diverse cellular functions, with GADD45 beta and GADD45 gamma showing marginal stability at physiological temperatures and GADD45 alpha demonstrating a higher stability and presence of amyloid-like aggregate properties. These unique characteristics may have important implications for their cytotoxicity and functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yuki Yoshikawa, Keisuke Yuzu, Naoki Yamamoto, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Tetsushi Iwasaki, Masatomo So, Yuji Goto, Atsuo Tamura, Eri Chatani
Summary: Amyloid fibrils play an important role in the development of various diseases, but their formation mechanisms are not fully understood. In this study, we investigated the formation of insulin B chain amyloid fibrils under different pH conditions and found that prefibrillar aggregates mediate amyloid nucleation. We also discovered different structures of amyloid fibrils at different pH values, indicating diverse pathways. Our findings provide insights into the nucleation mechanisms of amyloid fibrils.
Article
Pharmacology & Pharmacy
Camilla Thorlaksen, Adriana-Maria Stanciu, Martin Busch Neergaard, Nikos S. Hatzakis, Vito Fodera, Minna Groenning
Summary: Protein aggregates vary extensively in their morphological characteristics and have different biological outcomes. Producing samples with a specific morphology is challenging but important for studying the effects of aggregate characteristics. Various factors such as preparation conditions, post-treatment methods, salt concentration, and mechanical shaking can affect the morphology and integrity of amyloid-like fibrils and spherulites.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
News Item
Biochemistry & Molecular Biology
Urszula Nowicka, Min-Ji Kim, Agnieszka Chacinska
Summary: Research shows that modulating mitochondrial protein import activity can stimulate the clearance of protein aggregates in the cytosol, affecting cytosolic proteostasis and its collapse observed in neurodegenerative diseases.
Article
Biochemistry & Molecular Biology
Oshin D. Warerkar, Niyati H. Mudliar, Tanya Ahuja, Sailee D. Shahane, Prabhat K. Singh
Summary: The development of an accurate and sensitive sensor for detecting amyloid plaques is crucial for early diagnosis of protein disorders like Alzheimer's disease. The use of hemicyanine-based probe LDS730, belonging to the Near-Infrared Fluorescence family, provides higher precision, prevents photo-damage, and minimizes autofluorescence. The sensor exhibits a 110-fold increase in fluorescence turn-on emission when bound to insulin fibrils, making it highly sensitive with great potential for early detection and improving diagnostic accuracy.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Chetna Dhembla, Richa Arya, Ambrish Kumar, Suman Kundu, Monica Sundd
Summary: The L. major acyl carrier protein undergoes conformational changes at different pH levels, with an exposed Phe residue inducing amyloid formation. Phosphopantetheine inhibits amyloidogenesis in this protein.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Alexander G. Bobylev, Roman S. Fadeev, Liya G. Bobyleva, Margarita I. Kobyakova, Yuri M. Shlyapnikov, Daniil V. Popov, Ivan M. Vikhlyantsev
Summary: The study found that amyloid aggregates of smooth-muscle titin can impair cell adhesion and lead to cell death. The surface roughness may be a key factor contributing to the highly antiadhesive properties. The negative impact of amyloid aggregates on cell adhesion is likely intrinsic to other amyloid proteins with similar structure and properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mikhail Matveyenka, Stanislav Rizevsky, Dmitry Kurouski
Summary: Phosphatidylserine (PS) in the plasma membrane is crucial for cell signaling and apoptosis. The degree of unsaturation of fatty acids in PS affects the rate of insulin aggregation, and insulin aggregates formed in the presence of PS have lower toxicity compared to insulin fibrils in a lipid-free environment.
Article
Multidisciplinary Sciences
Fabian Wirth, Fabrice D. Heitz, Christine Seeger, Ioana Combaluzier, Karin Breu, Heather C. Denroche, Julien Thevenet, Melania Osto, Paolo Arosio, Julie Kerr-Conte, C. Bruce Verchere, Francois Pattou, Thomas A. Lutz, Marc Y. Donath, Christoph Hock, Roger M. Nitsch, Jan Grimm
Summary: In patients with type 2 diabetes, an antibody that targets and clears aggregated forms of IAPP can protect beta cells and improve glucose control.
NATURE COMMUNICATIONS
(2023)
Meeting Abstract
Allergy
Terumi Midoro-Horiuti, Barun K. Choudhury, Rene Vinas, Cheryl S. Watson, Randall M. Goldblum
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2015)
Meeting Abstract
Allergy
Surendra Negi, Terumi Midoro-Horiuti, Chris Kearney, Randall M. Goldblum, Werner Braun
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Randall M. Goldblum, Bo Ning, Barbara M. Judy, Luis Marcelo F. Holthauzen, Julius van Bavel, Atsushi Kamijo, Terumi Midoro-Horiuti
MOLECULAR IMMUNOLOGY
(2016)
Meeting Abstract
Allergy
Youko Murakami, Barun K. Choudhury, Randall M. Goldblum, Terumi Midoro-Horiuti
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2017)
Article
Cell Biology
Shuichiro Endo, Daniel J. Hochman, Terumi Midoro-Horiuti, Randall M. Goldblum, Edward G. Brooks
CELLULAR IMMUNOLOGY
(2011)
Review
Allergy
Rana S. Bonds, Terumi Midoro-Horiuti
CURRENT OPINION IN ALLERGY AND CLINICAL IMMUNOLOGY
(2013)
Article
Environmental Sciences
Yoichi Nakajima, Randall M. Goldblum, Terumi Midoro-Horiuti
ENVIRONMENTAL HEALTH
(2012)
Article
Allergy
Zun Liu, Shikha Bhattacharyya, Bo Ning, Terumi Midoro-Horiuti, Edmund W. Czerwinski, Randall M. Goldblum, Andrew Mort, Christopher M. Kearney
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2010)
Article
Allergy
Ruby Tiwari, Surendra S. Negi, Benjamin Braun, Werner Braun, Anna Pomes, Martin D. Chapman, Randall M. Goldblum, Terumi Midoro-Horiuti
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2012)
Meeting Abstract
Allergy
Terumi Midoro-Horiuti, Xiang Zhang, Yoichi Nakajima, Yueqing Zhang, Deepthi Kolli, Shuk-Mei Ho, Randall M. Goldblum
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2013)
Letter
Allergy
Randall M. Goldblum, Bo Ning, Mark A. Endsley, D. Mark Estes, Barbara M. Judy, Julius van Bavel, Terumi Midoro-Horiuti
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Randall M. Goldblum, Rumali S. Madagoda-Desilva, Yueqing Zhang, Julius van Bavel, Terumi Midoro-Horiuti
MOLECULAR IMMUNOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Rana Bonds, Guanjan S. Sharma, Yasuto Kondo, Jay van Bavel, Randall M. Goldblum, Terumi Midoro-Horiuti
MOLECULAR IMMUNOLOGY
(2019)
Article
Biochemical Research Methods
Mark L. Sowers, Hui Tang, Bing Tian, Randall Goldblum, Terumi Midoro-Horiuti, Kangling Zhang
JOURNAL OF PROTEOME RESEARCH
(2020)