Article
Biochemistry & Molecular Biology
Masahiro Sato, Nami Sato-Yamamoto, Ai Wakita, Misako Haraguchi, Manabu Shimonishi, Hiroyuki Okuno
Summary: In this study, the researchers injected AAV-6 vectors into the ampulla region of oviducts in pregnant female mice and found that it was the most effective method for transducing zona pellucida-enclosed preimplantation embryos in situ. This could be a useful tool in the genetic manipulation of early embryos and oviductal epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Environmental
Dongdan Yuan, Lei Zheng, Yong-Xin Liu, Hongguang Cheng, Aizhong Ding, Xiaomin Wang, Qiuyang Tan, Xue Wang, Yuzi Xing, En Xie, Haoming Wu, Shanyun Wang, Guibing Zhu
Summary: Through the use of double tracer technique and metagenomic sequencing, we found that the production of N2O in plateau wetlands is promoted by nitrifier denitrification, with the key functional genes belonging to comammox bacteria Nitrospira nitrificans. The comammox bacterial species N. nitrificans and ammonia oxidizing bacterial species Nitrosomonas europaea cooperate to produce N2O in plateau wetland sediments. Environmental factors such as elevation and total organic matter influence the cooperation pattern via N. nitrificans, thus affecting N2O production activity in plateau wetland sediments.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Cell Biology
Raquel Perez-Palacios, Maria Climent, Javier Santiago-Arcos, Sofia Macias-Redondo, Martin Klar, Pedro Muniesa, Jon Schoorlemmer
Summary: YY2, a mammalian-specific transcription factor, is expressed during mouse preimplantation development but its presence seems to be dispensable until the blastocyst stage in vitro. Depletion of Yy2 in mESCs results in increased levels of Zscan4 expression but does not affect the expression of TEs or other pluripotency markers.
Review
Cell Biology
Vallari Shukla, Miya Kudo Hoffding, Eva R. Hoffmann
Summary: Genome diversity is crucial for evolution and human health, with humans experiencing higher rates of congenital disorders. Gender differences influence DNA repair, reproductive capacity, and genome variation during embryonic development.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cheng Zhao, Savana Biondic, Katherine Vandal, Asa K. Bjoerklund, Michael Hagemann-Jensen, Theresa Maria Sommer, Jesica Canizo, Stephen Clark, Pascal Raymond, Daniel R. R. Zenklusen, Nicolas Rivron, Wolf Reik, Sophie Petropoulos
Summary: The preconceptual, intrauterine, and early life environments can significantly impact the developmental trajectories and health outcomes of offspring. Assisted reproductive technologies using additives may alter blastocyst formation and affect the molecular profile and physiology of the embryo.
Review
Developmental Biology
Franchesca D. Houghton
Summary: The development of preimplantation embryos relies on various nutrients in the reproductive tract, including oxygen which plays a crucial role in cellular physiology. Inadequate oxygen triggers a hypoxic response leading to a switch to glycolytic metabolism in cells, with ongoing debate about the optimal oxygen tension for embryo culture. Research using human embryonic stem cells can help understand the benefits of culturing embryos under physiological oxygen tensions.
Article
Endocrinology & Metabolism
Zhanhui Ou, Yu Deng, Yunhao Liang, Zhiheng Chen, Ling Sun
Summary: This study demonstrates the feasibility of using NGS for conducting PGT for thalassemia in affected embryos. The NGS analysis produced consistent results with prenatal diagnosis and gene analysis, and resulted in high clinical pregnancy and live birth rates.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Paula Gonzalez-Figueroa, Jonathan A. Roco, Ilenia Papa, Lorena Nunez Villacis, Maurice Stanley, Michelle A. Linterman, Alexander Dent, Pablo F. Canete, Carola G. Vinuesa
Summary: Regulatory T cells produce neuritin to suppress B cell-driven autoimmunity and IgE-mediated allergies. Lack of these T cells or neuritin leads to the emergence of autoantibodies and excessive IgE.
Article
Cell Biology
Sandra Alonso-Alonso, Anna Esteve-Codina, Beatriz Martin-Mur, Lucia Alvarez-Gonzalez, Aurora Ruiz-Herrera, Josep Santalo, Elena Ibanez
Summary: Research has found that the derivation rate of embryonic stem cells from single blastomeres of 8-cell mouse embryos is lower, and there are differences in the ability of sister blastomeres to produce ESCs. Although their non-pluripotent fate can be redirected through culture conditions and cell plasticity, at least half of the blastomeres have a lower potential to generate ESCs. Lines originated from the same embryo segregate into two groups based on transcriptional signatures.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Correction
Multidisciplinary Sciences
Hilal Gueralp, Kai O. Skaftnesmo, Erik Kjaerner-Semb, Anne Hege Straume, Lene Kleppe, Ruediger W. Schulz, Rolf B. Edvardsen, Anna Wargelius
Summary: The amended version of this paper has been published and can be accessed through a link at the top.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Hyungseok Seo, Edahi Gonzalez-Avalos, Wade Zhang, Payal Ramchandani, Chao Yang, Chan-Wang J. Lio, Anjana Rao, Patrick G. Hogan
Summary: BATF and IRF4 cooperate to counter T cell exhaustion in mouse tumor models, promoting better tumor control by CAR T cells.
Article
Pathology
Devin Cao, Michael G. Strainic, Daniel Counihan, Shiva Sridar, Fengqi An, Wasim Hussain, Alvin H. Schmaier, Marvin Nieman, M. Edward Medof
Summary: According to the classical view, the coagulation system functions in plasma to maintain hemostasis. However, experimental evidence suggests that vascular endothelial cells (ECs) endogenously synthesize coagulation factors during angiogenesis, promoting the mitotic function of VEGF-A.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Reproductive Biology
Zuzana Sefcikova, Janka Babelova, Veronika Kovarikova, Juraj Koppel, Dusan Fabian
Summary: The study indicates that preimplantation embryos from overweight mice may be more susceptible to oxidative stress compared to those from control females.
REPRODUCTIVE TOXICOLOGY
(2021)
Article
Cell Biology
Ilena Vincenti, Nicolas Page, Karin Steinbach, Alexander Yermanos, Sylvain Lemeille, Nicolas Nunez, Mario Kreutzfeldt, Bogna Klimek, Giovanni Di Liberto, Kristof Egervari, Margot Piccinno, Ghazal Shammas, Alexandre Mariotte, Nicolas Fonta, Nicolas Liaudet, Danielle Shlesinger, Anna Rita Liuzzi, Ingrid Wagner, Cynthia Saadi, Christine Stadelmann, Sai Reddy, Burkhard Becher, Doron Merkler
Summary: The study reveals that tissue-resident memory T cells (T-RM) may initiate CNS inflammation and immunopathology in chronic inflammatory diseases of the central nervous system (CNS), independent of circulating CD8(+) T cells. In the absence of CD4(+) T cells, CD8(+) T cells fail to expand and differentiate into terminal effectors. This sheds light on why inflammatory processes may evade current immunomodulatory treatments in chronic autoimmune CNS conditions.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Tomoaki Otsuka, Hiromi Shimojo, Hiroshi Sasaki
Summary: During the first stage of cell differentiation in mouse development, the Hippo signaling pathway regulates the differentiation of the trophectoderm and inner cell mass. The localization of Yes-associated protein 1 (YAP) in embryos is established in a position-dependent manner at the 32-cell stage. YAP localization in daughter cells is influenced by the YAP localization in mother cells, and gradually changes to the final pattern.
DEVELOPMENT GROWTH & DIFFERENTIATION
(2023)
Article
Environmental Sciences
Ziyi Wang, Kottawattage S. A. Kottawatta, Suranga P. Kodithuwakku, Thevarathanthrige S. Fernando, Yin-Lau Lee, Ernest H. Y. Ng, William S. B. Yeung, Kai-Fai Lee
Summary: Mancozeb and its metabolite ETU have been shown to inhibit embryo attachment, reduce cell viability, and alter gene expression profiles.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Oncology
Sudini R. Fernando, Cheuk-Lun Lee, Benancy P. C. Wong, Kiu-Wai Cheng, Yin-Lau Lee, Ming-Chung Chan, Ernest H. Y. Ng, William S. B. Yeung, Kai-Fai Lee
Summary: Our research indicates that estrogen and progesterone regulate the expression of PDIA1, leading to differential expressions of membrane PDIA1 protein to modulate endometrial receptivity. This suggests that membrane PDIA1 expression could potentially be used as a predictor of endometrial receptivity and embryo implantation in women undergoing assisted reproduction treatment.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Zhukang Guo, Baijiang Jin, Yile Fang, Yan Deng, Zhu Chen, Hui Chen, Song Li, Franklin Wang-Ngan Chow, Polly H. M. Leung, Hanming Wang, Lei Cai, Nongyue He
Summary: Since the concept of aptamer emerged, extensive research has been conducted, but there is a lack of practical applications for nucleic acids aptamer targeting cells. The complexity and irreproducibility of aptamer screening experiments are major obstacles. In response to this, the research group has developed an automated screening instrument for tumor cell nucleic acid aptamers and comprehensively characterized their properties.
CHINESE CHEMICAL LETTERS
(2022)
Article
Food Science & Technology
Yuan Liu, Yan Deng, Song Li, Franklin Wang-Ngai Chow, Mei Liu, Nongyue He
Summary: Food safety, especially the issue of antibiotic residues in animal derived foods, has been a major concern for public health. Conventional techniques for monitoring and detecting antibiotics have limitations, but the advent of aptamers has provided a new approach. Aptasensors based on nanomaterials have shown great potential for the monitoring and detection of antibiotic residues, and extensive research is being conducted in this field.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Immunology
Walton Chan, Franklin Wang-Ngai Chow, Chi-Ching Tsang, Xueyan Liu, Weiming Yao, Tony Tat-Yin Chan, Gilman Kit-Hang Siu, Alex Yat-Man Ho, Kristine Shik Luk, Susanna Kar-Pui Lau, Patrick Chiu-Yat Woo
Summary: This research characterized the impact of extracellular vesicles (EVs) derived from Candida auris on amphotericin B resistance. The EVs significantly increased the survival of the strain against amphotericin B, but had no effect on other antifungal drugs. Mass spectrometry analysis revealed the presence of immunogenic and drug resistance-associated proteins in the EVs. These findings provide important insights into the treatment of multidrug-resistant Candida auris.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Biochemistry & Molecular Biology
Abebe Mekuria Shenkutie, Jiaying Zhang, Mianzhi Yao, Daniel Asrat, Franklin W. N. Chow, Polly H. M. Leung
Summary: Suboptimal doses of antibiotics can promote biofilm formation and antibiotic resistance by regulating gene expression. Specifically, sub-MIC doses of imipenem and colistin upregulate genes associated with matrix synthesis, multidrug efflux pump, and fimbrial proteins, leading to increased biofilm production and antibiotic tolerance. Meanwhile, these antibiotics downregulate genes involved in protein synthesis, resulting in slowed cell metabolism and enhanced antibiotic tolerance in biofilm cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Zhu Chen, Kaixuan Zhao, Boyu Tan, Zengrui Tong, Ziyu He, Xiaofang Luo, Lei Cai, Hanming Wang, Polly H. M. Leung, Franklin Wang-Ngai Chow, Hui Chen, Yan Deng
Summary: This paper reports a method for HSV type testing, which includes selection, design, and synthesis of specific primers, validation of amplification products by sequencing and analysis, and optimization of reaction system and PCR reaction program by orthogonal design and sensitivity testing. Results showed that this method has great potentials for HSV type testing, with a lowest detection limit of approximately 6.67 x 10(6) copies/ml, and high specificity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Luhan Jiang, Dandan Cao, William S. B. Yeung, Kai-Fai Lee
Summary: This study used single-cell transcriptomics to uncover the cellular characteristics of mouse endometrial stromal cells (ESCs) and their role in modulating the microenvironment of a receptive endometrium. The results showed that both decidualized and non-decidualized ESCs are involved in extracellular remodeling and play a role in regulating endometrial receptivity and immune tolerance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Biochemistry & Molecular Biology
Franklin Wang-Ngai Chow
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Analytical
Meiling Wang, Hongna Liu, Jie Ren, Yunqi Huang, Yan Deng, Yuan Liu, Zhu Chen, Franklin Wang-Ngai Chow, Polly Hang-Mei Leung, Song Li
Summary: Infectious diseases and tumors are major medical challenges in the 21st century, driven by various factors such as population growth, aging, climate change, and genetic predispositions. Nucleic acid amplification technologies (NAATs) provide rapid and accurate diagnostic testing, offering critical information for better treatment and prognosis. NAATs are widely used due to their high sensitivity, specificity, and rapid amplification and detection. This review compares and describes common enzymes used in NAATs and summarizes the advances and common issues of NAATs in clinical application.
Article
Infectious Diseases
Abebe Mekuria Shenkutie, Daniel Gebrelibanos, Mianzhi Yao, Gadissa Bedada Hundie, Franklin W. N. Chow, Polly H. M. Leung
Summary: This study reveals the regulatory role of sRNA00203 in biofilm formation and antibiotic resistance in Acinetobacter baumannii, showing that its deletion significantly decreases the biofilm biomass and increases susceptibility to imipenem and ciprofloxacin. Additionally, the absence of sRNA00203 leads to the downregulation of genes involved in biofilm matrix synthesis, efflux pump production, lipopolysaccharide biosynthesis, and preprotein translocase subunit.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Immunology
Wing-Gi Wu, Marcus Ho-Hin Shum, Ivan Tak-Fai Wong, Kelvin Keru Lu, Lam-Kwong Lee, Jake Siu-Lun Leung, Hiu-Yin Lao, Annie Wing-Tung Lee, Pak-Ting Hau, Chloe Toi-Mei Chan, Harmen Fung-Tin Wong, Sharon Ka-Yee Fung, Sally Choi-Ying Wong, Iain Chi-Fung Ng, Timothy Ting-Leung Ng, Ning Chow, Alex Yat-Man Ho, Mei Fan Hung, Franklin Wang-Ngai Chow, Maureen Mo-Lin Wong, Wing-Kin To, Tommy Tsan-Yuk Lam, Kristine Shik Luk, Gilman Kit-Hang Siu
Summary: Between January 2015 and October 2022, 38 patients with melioidosis were identified in the KW Region, Hong Kong. Among them, 30 were found in the SSP district, covering an area of 2.5 km(2). An environmental investigation was conducted after an increase in cases in August to October 2022. Burkholderia pseudomallei was detected in air and soil samples, suggesting widespread distribution in the soil environment. Satellite imagery showed a reduction in vegetation in the SSP district, supporting the hypothesis of inhalation of aerosols from contaminated soil during extreme weather events. Clinicians should be aware of melioidosis during typhoon season and initiate appropriate investigation and treatment for patients with compatible symptoms.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Immunology
Carsten Tsun-Ka Kwok, Franklin Wang-Ngai Chow, Karry Yuen-Ching Cheung, Xiao-Yi Zhang, Daniel Kam-Wah Mok, Yiu-Wa Kwan, Gabriel Hoi-Huen Chan, George Pak-Heng Leung, Ka-Wang Cheung, Simon Ming-Yuen Lee, Ning Wang, Jing-Jing Li, Sai-Wang Seto
Summary: Using UPLC-Orbitrap-mass spectrometry, the chemical composition of theMT water extract was analyzed and eight abundant compounds were identified. The results showed that MT water extract could suppress inflammation by modulating macrophage polarization and inhibiting MAPKs pathways. It also demonstrated antibacterial activity against Staphylococcus aureus.
INFLAMMOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Franklin Wang-Ngai Chow, Russell M. Morphew
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Nanoscience & Nanotechnology
Jin Yang, Hongna Liu, Wenjing Pan, Mengru Song, Yutong Lu, Franklin Wang-Ngai Chow, Polly Hang-Mei Leung, Yan Deng, Masahi Hori, Nongyue He, Song Li
Summary: This article discusses the importance of the major histocompatibility complex (MHC) in humans and the rapid and accurate HLA typing technology established for preventing graft-verses-host disease (GVHD) in organ transplantation. The article also summarizes the most widely used sequencing typing platforms and the latest developments in HLA typing kits.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2022)