Article
Multidisciplinary Sciences
Pauline Okemo, Hao Long, Yen Cheng, Sagadevan Mundree, Brett Williams
Summary: Resurrection plants like Tripogon loliiformis suppress plant PCD via trehalose-mediated autophagy pathways during drying, and accumulate stachyose without inducing plant apoptotic-like cell death. These findings provide insights on the regulation of sugar metabolism and PCD pathways in resilient plants.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Ramon de Koning, Gertjan E. Wils, Raphael Kiekens, Luc De Vuyst, Geert Angenon
Summary: Due to climate change, farmers will face more extreme weather conditions and hence will need crops that are better adapted to these challenges. This study presents new insights into the role of raffinose family oligosaccharides (RFOs) as a resilience mechanism against environmental stress in common bean, particularly focusing on galactinol and RFOs in common beans exposed to drought and salt stress conditions.
Article
Genetics & Heredity
Donghao Wang, Zixuan Liu, Yue Qin, Shihao Zhang, Lulu Yang, Qiqi Shang, Xianling Ji, Youchao Xin, Xiaodong Li
Summary: This study demonstrates for the first time the role of MnGolS2 in biotic stress. It was also found that MnGolS2 may increase resistance to Botrytis cinerea by interacting with other resistance genes.
Article
Plant Sciences
Si Ma, Jianguo Lv, Xin Li, Tingting Ji, Zhenxian Zhang, Lihong Gao
Summary: The CsGolS4 gene in cucumber is significantly induced by both cold and drought stresses, with CsGolS4-overexpression plants showing higher RFO contents and suppressed ROS levels, leading to increased tolerance to cold and drought stresses. Conversely, CsGolS4-RNAi transgenic plants exhibit increased vulnerability under stress conditions.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Genetics & Heredity
Quankai Jing, Airu Chen, Zhaoyan Lv, Zhihao Dong, Lixia Wang, Xiaoke Meng, Yue Feng, Yu Wan, Chengyun Su, Yanjie Cui, Wenjuan Xu, Hualan Hou, Xiaobiao Zhu
Summary: This study identified four StGolS and nine StRFS genes in potato and classified them into three and five subgroups, respectively. Two StGolS and four StRFS genes were found to form collinear pairs with those in Arabidopsis and tomato. Further analysis revealed that StGolS4 showed high expression levels in transport-related tissues, PEG-6000, and ABA treatments, with significant upregulation under salt stress. Additionally, StRFS5 showed similar responses to StGolS4, while StRFS4 and StRFS8 gene expression significantly increased under salt treatment and decreased in PEG-6000 and ABA treatments. These findings lay a foundation for studying the functional characteristics and molecular mechanisms of these gene families in response to ABA, salt, and drought stresses, and provide new gene resources for breeding abiotic-stress-tolerant potatoes.
Article
Agronomy
Tanika Thakur, Kshitija Sinha, Tushpinder Kaur, Ritu Kapoor, Gulshan Kumar, Rupam Kumar Bhunia, Prafull Salvi
Summary: Rice is a crucial staple food crop, and an optimized rice transformation protocol is important for studying gene functions and improving rice crops.
Article
Plant Sciences
Gongxun Xu, Lijie Li, Jia Zhou, Deguo Lyu, Deying Zhao, Sijun Qin
Summary: Freezing injury in winter seriously affects plant growth and development. Different apple tree cultivars have different cold resistance strategies. The molecular mechanism of apple trees in response to freezing injury during winter dormancy is not well understood. In this study, physiological, biochemical, transcriptomics, and metabolomics analyses were conducted using cold-resistant and cold-sensitive cultivars to explore their cold resistance. The results revealed that the difference in key metabolic components is the major factor affecting their difference in cold resistance. The findings deepen our understanding of the cold resistance mechanism in apple trees.
HORTICULTURAL PLANT JOURNAL
(2023)
Review
Plant Sciences
Dinakaran Elango, Karthika Rajendran, Liza Van der Laan, Sheelamary Sebastiar, Joscif Raigne, Naveen A. Thaiparambil, Noureddine El Haddad, Bharath Raja, Wanyan Wang, Antonella Ferela, Kevin O. Chiteri, Mahendar Thudi, Rajeev K. Varshney, Surinder Chopra, Arti Singh, Asheesh K. Singh
Summary: Raffinose family oligosaccharides (RFOs) are widely distributed in the plant kingdom and have various beneficial effects in plants and humans. However, they are considered anti-nutritional factors for causing flatulence in humans and animals. RFOs provide tolerance to plant stress and have prebiotic potential in the human intestine.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agricultural Engineering
Ling Liu, Xiaolong Wu, Weibo Sun, Xiang Yu, Taku Demura, Dawei Li, Qiang Zhuge
Summary: Plants respond to abiotic stress through transcriptional and metabolic adjustments, with Raffinose family oligosaccharides (RFOs) and the key enzyme galactinol synthase (GolS) playing crucial roles. The GolS of Populus trichocarpa shares common features with GolS proteins in other plants and contains stress-responsive elements in its promoter region. Overexpressing AtGolS2 and PtrGolS3 in poplar enhances stress tolerance and increases the accumulation of stress-related metabolites, suggesting these genes as potential candidates for improving abiotic stress tolerance in woody plants.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Jun Yang, Chengcheng Ling, Yunyan Liu, Huamin Zhang, Quaid Hussain, Shiheng Lyu, Songhu Wang, Yongsheng Liu
Summary: The study comprehensively analyzed the RFS and GolS gene families in kiwifruit and revealed their roles in abiotic and biotic stress responses. Transcriptomic analysis and functional experiments confirmed the importance of some crucial genes, and identified one key gene (AcRFS4) that showed significantly increased transcription levels under salt stress and enhanced salt tolerance in kiwifruit.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Huilin Zhang, Junjie Wu, Wei Zhang, Manzhu Bao
Summary: This study reveals that CBF/DREB1 can regulate the accumulation of galactinol in Petunia hybrida through PhDREB1F and PhDREB1I, which enhances the plant's tolerance to cold stress. It is also found that PhDREB1F/1I can activate the expression of PhZFP1 and PhGolS1-1, leading to the accumulation of galactinol. These findings provide new insights into the molecular mechanism of plant responsiveness to cold stress mediated by CBF/DREB1 transcription factors.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Huilin Zhang, Zheng Sun, Shan Feng, Junwei Zhang, Fan Zhang, Wenen Wang, Huirong Hu, Wei Zhang, Manzhu Bao
Summary: PhZFP1 contributes to cold stress tolerance in petunia by regulating the synthesis of galactinol. This study provides new insights into the mechanisms of C2H2 zinc finger proteins in cold stress tolerance and identifies a candidate gene for improving cold stress tolerance.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Sakari Valimaki, Caroline Teyssier, Mikko Tikkinen, Armelle Delile, Nathalie Boizot, Saila Varis, Marie-Anne Lelu-Walter, Tuija Aronen
Summary: Vegetative propagation offers opportunities for forest regeneration by multiplying elite tree progeny. For Norway spruce, seed multiplication through somatic embryogenesis is the most efficient method. Filter disc cultures can improve the proliferation of embryogenic tissue (ET), although it is more dependent on ET selection and quantity.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Qianqian Guo, Xia Li, Li Niu, Paula E. Jameson, Wenbin Zhou
Summary: The study emphasizes the multifaceted response of plants to drought and cold stress, highlighting physiological and genetic changes that occur during acclimation to these stresses; plants experience a decline in photosynthetic capacity under both drought and cold stress, but can recover from the stress, with internal ABA playing a key role in plant acclimation to cold.
Article
Plant Sciences
Yirui Guo, Qianchao Wang, Hui Zhang, Tingzhi Huang, Xuesong Zhang, Heng Xie, Junna Liu, Ping Zhang, Li Li, Peng Qin
Summary: In this study, metabolomics and transcriptomics analysis were conducted to investigate the differences between waterlogging-resistant and non-waterlogging-resistant quinoa varieties. The results showed that waterlogging-resistant varieties had increased expression of genes related to saccharides and alcohols, as well as the ability to regulate galactinol and raffinose content. Compared to non-waterlogging-resistant varieties, waterlogging-resistant varieties exhibited better adaptation to waterlogging stress, which may provide a basis for breeding and identifying waterlogging-resistant quinoa varieties.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Joanna Szablinska-Piernik, Leslaw B. Lahuta
Summary: The study found that pea plants adjust to progressive soil drought by accumulating osmolytes and osmoprotectants, and transferring some of them (proline, sucrose, myoinositol) to the shoot tip, thereby protecting the youngest tissues from damage caused by water deficit.
JOURNAL OF PLANT PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Leslaw Bernard Lahuta, Joanna Szablinska-Piernik, Marcin Horbowicz
Summary: In peas, proline, GABA, branched-chain amino acids, and hydroxyproline accumulated under drought and decreased after re-watering. The first drought/re-watering cycle enhanced the plant's ability to return to a normal metabolic profile after subsequent stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Joanna Plonka, Joanna Szablinska-Piernik, Boguslaw Buszewski, Irena Baranowska, Leslaw B. Lahuta
Summary: The conditions for determining the antioxidant properties of cyclitols, flavanones, and glutathione have been identified. The low antioxidative properties of cyclitols compared to flavanones and glutathione were revealed. However, the methyl-ether derivatives of cyclitols showed a significant increase in the antioxidative properties of glutathione.
Article
Biochemistry & Molecular Biology
Leslaw Bernard Lahuta, Joanna Szablinska-Piernik, Katarzyna Glowacka, Karolina Stalanowska, Viorica Railean-Plugaru, Marcin Horbowicz, Pawel Pomastowski, Boguslaw Buszewski
Summary: This study analyzed the effects of bio-synthesized silver nanoparticles on wheat germination, early seedling development, and metabolic profiles of different tissues. It found that silver nanoparticles inhibited the growth of coleoptile and affected the concentration of metabolites in roots, coleoptile, and endosperm.
Article
Nutrition & Dietetics
Agata Rolnik, Beata Olas, Joanna Szablinska-Piernik, Leslaw Bernard Lahuta, Andrzej Rynkiewicz, Piotr Cyganski, Katarzyna Socha, Leszek Gromadzinski, Michael Thoene, Michal Majewski
Summary: The study investigated the effect of myo-inositol as a possible anti-inflammatory/antioxidant agent on human plasma and rat thoracic arteries. The results showed that myo-inositol had a protective effect, improving the response of impaired arteries and reducing oxidative stress.
Article
Plant Sciences
Joanna Szablinska-Piernik, Leslaw Bernard Lahuta, Karolina Stalanowska, Marcin Horbowicz
Summary: This study investigated the effects of silver nanoparticles and silver ions on pea seeds and seedlings. The results showed that both silver nanoparticles and silver ions had no negative impact on seed germination and even promoted seedling growth. However, high concentrations of silver ions exhibited significant inhibitory effects on seed germination and seedling growth, causing morphological deformations. Additionally, the presence of silver ions also led to changes in plant metabolites.
Article
Agronomy
Magdalena Gawlowska, Leslaw Lahut, Lech Boros, Aneta Sawikowska, Pankaj Kumar, Michal Knopkiewicz, Zygmunt Kaczmarek, Wojciech Swiecicki
Summary: This study identified multiple important traits related to field pea through phenotypic measurements and marker validation. This has significant implications for breeding and improving yield and quality of field pea.
Article
Biochemistry & Molecular Biology
Leslaw Bernard Lahuta, Joanna Szablinska-Piernik, Karolina Stalanowska, Katarzyna Glowacka, Marcin Horbowicz
Summary: The phytotoxicity of silver nanoparticles (Ag NPs) to plant seeds and seedlings depends on the properties and concentration of the nanoparticles, as well as the species and stress tolerance of the plants. In this study, citrate-stabilized spherical Ag NPs of different sizes were found to have no effect on the germination of wheat grains, but smaller nanoparticles decreased the growth of seedling roots. Significant rearrangements in carbohydrate profiles were observed in the seedlings, while most other polar metabolites remained unaffected.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tomasz Antonowski, Adam Osowski, Damian Szczesny, Joanna Szablinska-Piernik, Jerzy Juskiewicz, Leslaw Lahuta, Andrzej Rynkiewicz, Joanna Wojtkiewicz
Summary: This study investigated the concentration change of myo-inositol solution in the serum of Wistar rats after oral administration. The results showed that the myo-inositol concentration reached its peak in the first hour and then rapidly declined. However, even after 24 hours, the concentration remained higher than before administration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Marian Saniewski, Joanna Szablinska-Piernik, Agnieszka Marasek-Ciolakowska, Joanna Mitrus, Justyna Goraj-Koniarska, Leslaw B. Lahuta, Wieslaw Wiczkowski, Kensuke Miyamoto, Junichi Ueda, Marcin Horbowicz
Summary: The accumulation of anthocyanins in detached leaves and excised stems of Kalanchoe blossfeldiana under natural light conditions with or without methyl jasmonate (JA-Me) was investigated. Anthocyanins were found to accumulate on the abaxial side of the leaves when the adaxial surface was held lower than the abaxial surface, regardless of the growth stage and photoperiod. The application of JA-Me in lanolin paste inhibited the accumulation of anthocyanins in both the detached leaves and excised stems. Possible mechanisms and accompanying changes in primary metabolites and histology were described.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Piotr Androsiuk, Sylwia Eryka Milarska, Justyna Dulska, Wioleta Kellmann-Sopyla, Joanna Szablinska-Piernik, Leslaw Bernard Lahuta
Summary: In this study, 13 Avena species were compared for polymorphism using iPBS markers and soluble carbohydrate profiles. The iPBS markers generated 83 bands, with 20.5% exhibiting polymorphism. No species-specific bands were found. Low genetic diversity was observed, with the highest values for A. nuda. The clustering analysis and PCoA results showed the main criterion for grouping to be the polyploidy level. High-resolution gas chromatography revealed similar compositions of soluble carbohydrates, but significant differences in content among the studied samples. The pattern of interspecific variation in soluble carbohydrates was consistent with the iPBS marker results, providing valuable data for characterizing Avena resources and discussing the evolution of this genus.
JOURNAL OF APPLIED GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Joanna Szablinska-Piernik, Leslaw Bernard Lahuta
Summary: This study investigated whether wheat plants can resume metabolism after short-term cyclical periods of drought and if the reaction to subsequent stress is the same. The results revealed an accumulation of sugars, proline, and malic acid after short-term drought, but the concentrations decreased during recovery. After the second drought, the concentrations of certain metabolites were higher, and the total polar metabolite concentration after rewatering was elevated. These findings confirm the involvement of proline and highlight the responsiveness of soluble carbohydrate metabolism to stress/recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Leslaw B. Lahuta, Ryszard J. Gorecki, Joanna Szablinska-Piernik, Marcin Horbowicz
Summary: This study aimed to determine the carbohydrate profile in specific organs (roots, hypocotyl, and cotyledons) of common buckwheat seedlings and investigate the similarities in carbohydrate accumulation in response to cold stress and dehydration. The results showed varied saccharide compositions in the different organs of common buckwheat seedlings. The highest concentrations of cyclitols, raffinose, and stachyose were found in the hypocotyl, indicating possible transport from cotyledons. Accumulation of raffinose and stachyose was a strong indicator of the response to cold stress and dehydration in all buckwheat organs.
Article
Plant Sciences
Leslaw B. Lahuta, Joanna Szablinska-Piernik, Karolina Stalanowska, Marcin Horbowicz, Ryszard J. Gorecki, Viorica Railean, Pawel Pomastowski, Boguslaw Buszewski
Summary: Plant responses to stresses caused by metal nanoparticles are affected by cyclitols, which are absorbed and transported within the plant. Cyclitols induce the accumulation of sucrose and 1-kestose in seedlings, while (Bio)Ag NPs increase the concentrations of these sugars. Additionally, cyclitols decrease the concentrations of fructose and glucose. Similar effects were observed in seedlings developed from primed grains.
Article
Biochemistry & Molecular Biology
Karolina Stalanowska, Joanna Szablinska-Piernik, Adam Okorski, Leslaw B. Lahuta
Summary: The present study reveals the size-dependent harmful effect of zinc oxide nanoparticles (ZnO NPs) on the growth of pea and wheat seedlings. The metabolic response, including the increase of sucrose and GABA and the changes of metabolites like citrate and malate, is common for all tested cultivars of pea and wheat under the exposure of ZnO NPs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
