Article
Chemistry, Multidisciplinary
Xiaohong Pan, Danyue Nie, Xueping Guo, Shanshan Xu, Dingyang Zhang, Fang Cao, Xiong Guan
Summary: TiO2 nanoparticles were synthesized under hydrothermal conditions and used as a green and efficient nanopesticide against bacterial wilt. The nanoparticles showed high antibacterial activity against Ralstonia solanacearum, damaging cell membranes and DNA, decreasing disease-causing gene expression, and reducing the levels of cellulose and pectinase. Moreover, the nanoparticles were found to attach to and accumulate in tomato plants, enhancing disease resistance and increasing the content of peroxidase and catalase.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Microbiology
Yaxing Su, Yanan Xu, Hailing Liang, Gaoqing Yuan, Xiaogang Wu, Dehong Zheng
Summary: This study identified key genes required for R. solanacearum survival in tomato plants using transposon insertion sequencing technology, highlighting the importance of genes related to cell wall/membrane/envelope biogenesis, amino acid transport and metabolism, energy production and conversion, posttranslational modification, protein turnover, and chaperones in the pathogenic mechanism of this bacterium.
Article
Plant Sciences
Raja Asad Ali Khan, Yuanyang Tang, Ishrat Naz, Syed Sartaj Alam, Wenzhao Wang, Musharaf Ahmad, Saba Najeeb, Crist Rao, Yanlin Li, Bingyan Xie, Yan Li
Summary: Matricaria chamomilla flower extract was used for synthesizing zinc oxide nanoparticles with antibacterial potential against Ralstonia solanacearum. In vitro and in vivo studies showed the effectiveness of the biosynthesized ZnONPs in controlling the bacterium and promoting plant growth.
Article
Biotechnology & Applied Microbiology
Zhiyu Fan, Yuxia Mei, Jiawei Xing, Tian Chen, Di Hu, Hui Liu, Yingjun Li, Derui Liu, Zufeng Liu, Yunxiang Liang
Summary: In this study, a novel detection method for Ralstonia solanacearum (RS) based on loop-mediated isothermal amplification (LAMP) and CRISPR/Cas12a was developed. This method can accurately and rapidly detect RS, and has potential application for the detection of tomato bacterial wilt in southern China. The detection process is simple, fast, and does not require professional laboratory equipment.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Dhananjay Kumar Yadav, Venkatappa Devappa, Abhijeet Shankar Kashyap, Narendra Kumar, V. S. Rana, Kumari Sunita, Dinesh Singh
Summary: Chemical mutagenic agents and ultraviolet irradiation were used to enhance the antagonistic property of Bacillus amyloliquefaciens DSBA-11 against Ralstonia solanacearum UTT-25 for managing tomato wilt disease effectively. The derivative strain MHNO2-20, treated with nitrous acid, showed the highest inhibition to R. solanacearum UTT-25. GC/MS analysis detected 18 major compounds in MHNO2-20, with compound 3-isobutyl hexahydropyrrolo (1,2), pyrazine-1,4-dione being the most abundant. Bio-efficacy assessment revealed that MHNO2-20 had the highest control efficacy (88.75%) against bacterial wilt disease in tomato plants.
Article
Biotechnology & Applied Microbiology
Jianlei Shi, Deju Shui, Shiwen Su, Zili Xiong, Wenshan Zai
Summary: In this study, the transcriptomes of resistant and susceptible tomato lines under control and inoculated conditions were analyzed, and several key genotype-specific hub genes involved in different biological processes were identified. These findings lay a foundation for a better understanding of the molecular basis by which resistant tomato lines respond to Ralstonia solanacearum.
Article
Plant Sciences
Fengfeng Dang, Jinhui Lin, Yajing Li, Ruoyun Jiang, Yudong Fang, Fei Ding, Shuilin He, Yanfeng Wang
Summary: Bacterial wilt is a severe disease of tomato caused by Ralstonia solanacearum, and this study reveals the crucial role of SlWRKY30 in regulating tomato resistance to the disease. SlWRKY30 overexpression reduces tomato susceptibility to bacterial wilt and increases H2O2 accumulation and cell necrosis, indicating its positive regulatory role in tomato resistance.
HORTICULTURE RESEARCH
(2023)
Article
Biochemical Research Methods
Cuihong Xu, Lingkun Zhong, Zeming Huang, Chenying Li, Jiazhang Lian, Xuefang Zheng, Yan Liang
Summary: A bioluminescent R. solanacearum strain was generated by expressing plasmid-based luxCDABE, and its light intensity was linearly related to bacterial concentrations. The light signals of this strain were found to be transported from roots to stems via vasculature in tomato and Arabidopsis. The quantification of light intensity accurately reflected the difference in disease resistance between Arabidopsis wild type and resistant mutants. This bioluminescent strain offers a tool for high-throughput study of R. solanacearum-Arabidopsis interaction in the future.
Article
Plant Sciences
Sushuang Liu, Qi Xue, Shuying Zhu, Yanmin Liu, Huasong Zou
Summary: This study identified 209 genes that showed altered expression patterns in roots infected with Ralstonia solanacearum, including the downregulation of the wall-associated receptor kinase WAKL20. Knockdown of WAKL20 resulted in impaired root development in tomato and Nicotiana benthamiana plants.
Article
Genetics & Heredity
Jianfang Qiu, Lei Ni, Xue Xia, Shihao Chen, Yan Zhang, Min Lang, Mengyu Li, Binman Liu, Yu Pan, Jinhua Li, Xingguo Zhang
Summary: This study conducted a comprehensive investigation of the SlPP2C gene family in tomato and found that these genes play important roles in tomato development, stress response, and phytohormone signaling. The study also revealed that gene duplication was a major factor in the expansion of SlPP2Cs, and confirmed their diverse expression patterns in different tomato tissues.
Article
Biochemistry & Molecular Biology
Rekha Gopalan-Nair, Marie-Francoise Jardinaud, Ludovic Legrand, David Landry, Xavier Barlet, Celine Lopez-Roques, Celine Vandecasteele, Olivier Bouchez, Stephane Genin, Alice Guidot
Summary: The study utilized experimental evolution to investigate the adaptive potential of Ralstonia solanacearum in overcoming the resistance of tomato cultivar Hawaii 7996. After over 300 generations of serial passaging, pathogen adaptation to the resistant cultivar's environment was observed without breakdown of plant resistance. Genomic and transcriptomic analyses revealed genetic alterations and convergent global rewiring of the virulence regulatory network in independently evolved clones.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Microbiology
Abdelmonim Ali Ahmad, Hardian Susilo Addy, Qi Huang
Summary: A jumbo phage RsoM2USA isolated from a tomato field in Florida, U.S., has a long latent period and short infection cycle. With a genome size of 343,806 bp, it is the largest Ralstonia-infecting phage sequenced to date, belonging to the family Myoviridae. It infects multiple Ralstonia species and significantly reduces the virulence of susceptible strains in tomato plants.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Leo Gerlin, Antoine Escourrou, Cedric Cassan, Felicia Maviane Macia, Nemo Peeters, Stephane Genin, Caroline Baroukh
Summary: The plant pathogen Ralstonia solanacearum proliferates rapidly in xylem vessels, leading to plant death at a tipping point in bacterial wilt dynamics. Metabolic and physiological changes during infection are similar to drought stress. Glutamine is identified as the preferred substrate for R. solanacearum during colonization.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiuyang Si, Hongyan Liu, Xi Cheng, Chengcui Xu, Zhanghui Han, Zhongren Dai, Rongqing Wang, Changtian Pan, Gang Lu
Summary: In this study, we conducted a comprehensive analysis of the transcriptional landscape in tomato resistant and susceptible lines upon Ralstonia solanacearum inoculation. The study revealed the involvement of noncoding RNA in tomato bacterial wilt disease and their interactions with mRNAs. Additionally, a ceRNA network was constructed to identify potential contributions of related genes to the response against bacterial inoculation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Horticulture
Yang Gao, Lei Wang, Rui Liu, Jihui Tian, Kunzheng Cai
Summary: This study evaluated the effects of wheat straw biochar on disease development, plant physiology, and proteomic expression in tomato plants infected with Ralstonia solanacearum. The results showed that biochar application improved plant growth, reduced disease severity, and decreased the colonization of R. solanacearum in tomato stems. Physiological analysis revealed that biochar treatment increased peroxidase and lipoxygenase activities, as well as the content of total soluble phenolics and lignin-like phenolic polymers in tomato leaves. Proteomic analysis showed that biochar treatment upregulated genes involved in lignin synthesis, anion channel and sulfur metabolism, and downregulated genes involved in microtubule and cytoskeleton organization, as well as UDP-glucose metabolism.
SCIENTIA HORTICULTURAE
(2023)
Article
Biotechnology & Applied Microbiology
K. M. Elhalag, N. A. S. Messiha, H. M. Emara, S. A. Abdallah
JOURNAL OF APPLIED MICROBIOLOGY
(2016)
Article
Plant Sciences
Shahenda M. A. Farag, Kamel M. A. Elhalag, Mohamed H. Hagag, Abdel Salam M. Khairy, Heba M. Ibrahim, Moheb T. Saker, Nevein A. S. Messiha
JOURNAL OF PHYTOPATHOLOGY
(2017)
Article
Virology
Abdelmonim Ali Ahmad, Kamel M. Elhalag, Hardian Susilo Addy, Mohamed A. Nasr-Eldin, Ahmed S. Hussien, Qi Huang
ARCHIVES OF VIROLOGY
(2018)
Article
Microbiology
Kamel Elhalag, Mohamed Nasr-Eldin, Ahmed Hussien, Abdelmonim Ahmad
JOURNAL OF BASIC MICROBIOLOGY
(2018)
Article
Agriculture, Multidisciplinary
Kamel Elhalag, Nader Elbadry, Shahenda Farag, Mohamed Hagag, Ahmed Hussien
JOURNAL OF PLANT DISEASES AND PROTECTION
(2020)
Article
Plant Sciences
Nevein A. S. Messiha, K. M. A. Elhalag, N. M. Balabel, H. A. Matar, S. M. A. Farag, M. H. Hagag, A. M. Khairy, M. M. Abd El-Aliem, M. S. Hanafy, N. S. Farag
Summary: This study examined the impact of microbial biodiversity in soil on potato brown rot suppression under different fertilisation regimes. It was found that fields with low organic matter and carbon nitrogen ratios were conducive to the disease, while fields rich in antagonistic actinomycetes showed suppression. Different fertilisation regimes had varied effects on disease control, with NPK suppression notably increasing eubacteria biodiversity. Various replacements for potassium also showed significant suppression of infection and changes in soil biodiversity. Overall, the recommended fertilisation regime to suppress potato brown rot disease depends on soil edaphic factors.
ARCHIVES OF PHYTOPATHOLOGY AND PLANT PROTECTION
(2021)
Article
Entomology
N. A. S. Messiha, K. M. A. Elhalag, N. M. Balabel, S. M. A. Farag, H. A. Matar, M. H. Hagag, A. M. Khairy, M. M. Abd El-Aliem, E. Eleiwa, O. M. E. Saleh, N. S. Farag
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
(2019)
Article
Entomology
Mohamed Nasr-Eldin, Nevein Messiha, Badawi Othman, Allam Megahed, Kamel Elhalag
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
(2019)