Article
Cell Biology
Maria P. Kotini, Miesje M. van der Stoel, Jianmin Yin, Mitchell K. Han, Bettina Kirchmaier, Johan de Rooij, Markus Affolter, Stephan Huveneers, Heinz-Georg Belting
Summary: Blood vessel morphogenesis is driven by coordinated endothelial cell behaviors. Active remodeling of cell-cell junctions promotes cellular plasticity while preserving vascular integrity. Lumen expansion in angiogenic sprouts is accompanied by the formation of transient finger-shaped junctions. Blood pressure positively regulates the formation of junctional fingers, while flow inhibition prevents their formation. Mechanotransduction protein vinculin is recruited to junctional fingers, and its genetic deletion prevents finger formation. Endothelial cells employ force-dependent junctional remodeling to counteract external forces and maintain vascular integrity during sprouting angiogenesis.
Article
Biochemical Research Methods
Paul M. Van Liedekerke, Lila M. Gannoun, Axelle M. Loriot, Tim M. Johann, Frederic P. M. Lemaigre, Dirk M. Drasdo
Summary: The formation of biliary lumens involves coordination of various cellular mechanisms such as cell division, polarization, and fluid secretion. Successful lumen formation requires contributions from directed cell division, local osmotic effects from salt excretion, and controlled differentiation of cells, with apical constriction playing a moderate role. Understanding these processes is crucial for identifying intervention points to address defective bile ducts in development or disease.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Changwei Li, Minglong Qiu, Leilei Chang, Jin Qi, Lianfang Zhang, Bernhard Ryffel, Lianfu Deng
Summary: This study identified USP26 as a regulator of bone homeostasis that coordinates bone formation and resorption. USP26 stabilizes beta-catenin to promote osteogenesis and inhibits osteoclastic differentiation by stabilizing inhibitors of NF-kappa B alpha. These findings highlight the osteoprotective effect of USP26 and suggest it as a potential therapeutic target for osteoporosis.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Biochemical Research Methods
Samhita P. Banavar, Michael Trogdon, Brian Drawert, Tau-Mu Yi, Linda R. Petzold, Otger Campas
Summary: Many cellular processes require cell polarization to be maintained during changes in shape, growth, or movement. The presence of mechanical feedback encoded in the Cell Wall Integrity pathway can help coordinate cell morphogenesis and polarity, ensuring polarization is maintained during cell shape changes.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Cell Biology
Arun A. Chandrakumar, Etienne Coyaud, Christopher B. Marshall, Mitsuhiko Ikura, Brian Raught, Robert Rottapel
Summary: Rab11 GTPase proteins play important roles in cytokinesis, ciliogenesis, and lumenogenesis; SH3BP5 and SH3BP5L are crucial for the activation of Rab11a and cyst lumen formation; Tankyrase regulates epithelial polarity and lumen formation by inhibiting SH3BP5 and SH3BP5L, which is counteracted by RNF146.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Qingmei Sun, Junqiu Shi, Hui Sun, Yunqing Zhu, Jianzhong Du
Summary: This review summarizes the design principles and preparation methods of membrane-compartmentalized and lumen-compartmentalized polymersomes, and discusses the recent advances of MCPs as nanoreactors and cell mimics. The future research directions of MCPs are also prospected.
Article
Multidisciplinary Sciences
Li-Ting Wang, Abira Rajah, Claire M. Brown, Luke McCaffrey
Summary: The short intracellular domain of CD13 protein is essential for capturing endosomes at the apical site and is crucial for cell polarization.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Carina Magdaleno, Trenton House, Jogendra S. Pawar, Sophia Carvalho, Narendiran Rajasekaran, Archana Varadaraj
Summary: This study explores the impact of fibronectin (FN) matrix on epithelial functions in the context of cancer progression, showing that FN fibril formation decreases during hypoxia, leading to reduced migratory potential. Additionally, disruption of FN fibrils in normal breast cells results in acinar luminal filling, indicating a loss of apicobasal polarity.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Martin Fischer, Amy E. Schade, Timothy B. Branigan, Gerd A. Mueller, James A. DeCaprio
Summary: Cell cycle-dependent gene transcription is tightly regulated by multiple complexes, controlling gene expression during G1/S and G2/M phases to fulfill different functions in processes such as DNA synthesis and mitosis.
TRENDS IN BIOCHEMICAL SCIENCES
(2022)
Article
Pathology
Prisca K. Lin, Gretchen M. Koller, George E. Davis
Summary: This study investigates the role of five growth factors (insulin, FGF-2, stem cell factor, IL-3, and stromal-derived factor 1a) in tube morphogenesis of human endothelial cells (ECs). The study found that different combinations of these factors play specific roles in EC lumen formation and sprouting behavior. Signaling analysis revealed the involvement of multiple promorphogenic signals and the inhibition of proregressive signals in these processes. Pharmacologic inhibition of specific kinases also showed selective effects on EC behavior.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Review
Cell Biology
Jarama Clucas, Pascal Meier
Summary: The receptor-interacting serine/threonine-protein kinase 1 (RIPK1) acts as a molecular switch to regulate cell survival, inflammation, and cell death. Recent studies have revealed the catalytic and non-catalytic roles of RIPK1, as well as its context-dependent functions in health and disease. RIPK1 is involved in the intricate signaling pathways that mediate cell death and inflammation, which are important components of the immune response. Dysregulation of RIPK1 has been associated with inflammatory disorders, autoimmune diseases, and cancer. Understanding the molecular mechanisms that govern RIPK1 and its roles in different cellular contexts can provide insights for developing therapeutic strategies targeting RIPK1.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jifeng Liu, Hongyan Long, Dagmar Zeuschner, Andreas F. B. Rader, William J. Polacheck, Horst Kessler, Lydia Sorokin, Britta Trappmann
Summary: Current tissue engineering strategies lack materials that promote angiogenesis. Here the authors develop a microfluidic in vitro model in which chemokine-guided endothelial cell sprouting into a tunable hydrogel is followed by the formation of perfusable lumens to determine the material properties that regulate angiogenesis.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Hongguang Wu, Dan He, Soma Biswas, Md Shafiquzzaman, Xin Zhou, Jean Charron, Yibin Wang, Bijaya K. Nayak, Samy L. Habib, Huijuan Liu, Baojie Li
Summary: Activation of the mTOR signaling pathway combined with inactivation of the p38MAPK-p53/p16 axis initiates the development of RCC from renal proximal tubules, uncovering previously unidentified mechanisms and potential therapeutic targets for RCC treatment.
Article
Biology
Lei Yin, Zhenggao Zheng, Yilin Li, Xiying Li, Dan Cheng, Chunxia Dong, Yixuan Liu, Jindong Zhao
Summary: Spatial periodic signal based on Turing's principle is used to simulate cell differentiation in multicellular organisms. In the filamentous cyanobacterium Nostoc sp. PCC 7120, a dividing cell delays differentiation until completion of division, resulting in only one daughter cell becoming heterocyst. Mutants of patU3, an inhibitor of heterocyst formation, do not show this delay and form heterocyst pairs or dumbbell-shaped heterocysts from dividing cells or cells undergoing cytokinesis. PatA mutant restores intercalary heterocysts by a single nucleotide mutation of patU3, and double mutants of patU3/patA and patU3/hetF exhibit patU3 mutant phenotypes. HetF, which degrades PatU3, is recruited to the cell divisome through its C-terminal domain. A HetF mutant lacking the C-terminal domain fails to prevent heterocyst pair formation, indicating the importance of divisome recruitment of HetF for delaying differentiation in dividing cells. This study highlights the key role of PatU3 in cell-division coupled control of differentiation.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Multidisciplinary Sciences
Daniele Kunz, Anfu Wang, Chon U. Chan, Robyn H. Pritchard, Wenyu Wang, Filomena Gallo, Charles R. Bradshaw, Elisa Terenzani, Karin H. Mueller, Yan Yan Shery Huang, Fengzhu Xiong
Summary: This study reveals that embryonic tissues in chicken undergo shape change due to mechanical input from the vitelline membrane. Experimental relaxation of the membrane during early development impairs blastoderm expansion, while maintaining membrane tension during later stages leads to stalled elongation, failure of neural tube closure, and axis rupture. Structural analysis suggests that the weakening of the membrane is associated with a reduction in outer-layer glycoprotein fibers, which is caused by increased albumen pH due to CO2 release from the egg.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Peng Xia, Daniel Guetl, Vanessa Zheden, Carl-Philipp Heisenberg
Article
Biochemistry & Molecular Biology
Shayan Shamipour, Roland Kardos, Shi-Lei Xue, Bjoern Hof, Edouard Hannezo, Carl-Philipp Heisenberg
Review
Biochemistry & Molecular Biology
Nicoletta Petridou, Carl-Philipp Heisenberg
Editorial Material
Cell Biology
Stefania Tavano, Carl-Philipp Heisenberg
NATURE CELL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Cornelia Schwayer, Shayan Shamipour, Kornelija Pranjic-Ferscha, Alexandra Schauer, Maria Balda, Masazumi Tada, Karl Matter, Carl-Philipp Heisenberg
Review
Cell Biology
Benoit G. Godard, Carl-Philipp Heisenberg
CURRENT OPINION IN CELL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Dorothee Bornhorst, Peng Xia, Hiroyuki Nakajima, Chaitanya Dingare, Wiebke Herzog, Virginie Lecaudey, Naoki Mochizuki, Carl-Philipp Heisenberg, Deborah Yelon, Salim Abdelilah-Seyfried
NATURE COMMUNICATIONS
(2019)
Article
Biology
Alexandra Schauer, Diana Pinheiro, Robert Hauschild, Carl-Philipp Heisenberg
Article
Multidisciplinary Sciences
Tony Y-C Tsai, Mateusz Sikora, Peng Xia, Tugba Colak-Champollion, Holger Knaut, Carl-Philipp Heisenberg, Sean G. Megason
Article
Developmental Biology
Alexandra Schauer, Carl-Philipp Heisenberg
Summary: During development, asymmetries are established at different scales, leading to the formation of different body structures. Traditional studies have focused on dissecting the role of signaling pathways and cellular rearrangements in intact embryos. Recent research using embryonic stem cells has provided new insights into the various ways by which embryos can be built and the impact of extrinsic factors.
DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Shayan Shamipour, Silvia Caballero-Mancebo, Carl-Philipp Heisenberg
Summary: The cytoplasm is a gel-like crowded environment composed of various macromolecules and organelles, with highly organized and heterogeneous structure that restricts the diffusive dynamics of molecules. Despite its crowded nature at the microscopic scale, large-scale reorganization of the cytoplasm is required for important cellular functions, such as cell division and polarization. The mechanisms by which the cytoskeleton drives cytoplasmic reorganization across different scales, structures, and species are only beginning to be understood.
DEVELOPMENTAL CELL
(2021)
Article
Cell Biology
Benoit G. Godard, Remi Dumollard, Edwin Munro, Janet Chenevert, Celine Hebras, Alex McDougall, Carl-Philipp Heisenberg
DEVELOPMENTAL CELL
(2020)
Review
Biophysics
Feyza Nur Arslan, Julia Eckert, Thomas Schmidt, Carl-Philipp Heisenberg
Summary: This review article focuses on the importance and research status of cell-cell adhesion, including descriptions and regulations at different levels, as well as discussions on the molecular and cellular mechanisms of cell-cell adhesion.
BIOPHYSICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Nicoletta Petridou, Bernat Corominas-Murtra, Carl-Philipp Heisenberg, Edouard Hannezo
Summary: Embryo morphogenesis is influenced by dynamic changes in tissue material properties, which resemble phase transitions. This study demonstrates that rigidity percolation theory can predict material/structural phase transitions in embryonic tissues, with zebrafish blastoderm undergoing a genuine rigidity phase transition due to changes in cell connectivity. By quantitatively predicting and experimentally verifying the characteristics of phase transitions, the study reveals that the uniform phase transition in blastoderm cells is dependent on specific cell divisions.
Article
Biology
Benoit G. Godard, Remi Dumollard, Carl-Philipp Heisenberg, Alex McDougall
Summary: In early ascidian embryos, cell geometry and polarity domains cooperate to position the cleavage plane during unequal cell division (UCD), determining the orientation and size of daughter cells.