Article
Chemistry, Analytical
Yupan Wu, Yuanbo Yue, Haohao Zhang, Xun Ma, Kemu Li, Wen Zeng, Shaoxi Wang, Yingqi Meng
Summary: In this study, a microfluidic device based on bipolar electrode (BPE) array is developed for assessing cell viability using AC electrodynamics. The viability of various cells can be evaluated by analyzing the electro-rotation (ROT) speed and direction of cells, as well as the dielectrophoresis (DEP) responses of cells. This study demonstrates the utility of BPEs in enabling scalable and high-throughput AC electrodynamics platforms for cell characterization and viability assessment.
ANALYTICA CHIMICA ACTA
(2023)
Review
Nanoscience & Nanotechnology
Geon Lee, Eui-Sang Yu, Yong-Sang Ryu, Minah Seo
Summary: This review discusses the development and integration of metasurface-assisted material sensing and transportation technologies. The trend of combining optical detection and transporting schemes beyond diffraction and diffusion limits is explored, and the potential for exceptional performance in molecular detections is highlighted.
Article
Chemistry, Analytical
Rino Takeuchi, Masato Suzuki, Tomoyuki Yasukawa
Summary: The electrorotation rates of K562 cells were estimated using a novel device to identify differentiation status without labeling. The rates increased with cell differentiation due to higher cytoplasm conductivity from increased iron ion concentrations for hemoglobin production. This method offers a non-invasive way to estimate cell differentiation stages.
ANALYTICAL SCIENCES
(2021)
Article
Chemistry, Analytical
Ina Turcan, Iuliana Caras, Thomas Gabriel Schreiner, Catalin Tucureanu, Aurora Salageanu, Valentin Vasile, Marioara Avram, Bianca Tincu, Marius Andrei Olariu
Summary: This study utilized dielectrophoresis and electrical impedance spectroscopy for the detection and electrical characterization of cancer cell lines and primary tumor cells, allowing for rapid and label-free differentiation of various cancer cells from normal ones. Through dielectrophoresis trapping and electrical impedance spectroscopy analysis of dielectric characteristics, the different types of cells were clearly differentiated based on their crossover frequencies. The differences between cancerous and normal cells were highlighted using a Randles equivalent circuit model in terms of a series group of charge transport resistance and constant phase element.
Article
Chemistry, Analytical
Gaurav Pendharkar, Yen-Ta Lu, Chia-Ming Chang, Meng-Ping Lu, Cheng-Hsien Liu
Summary: This study introduces an insulator-based dielectrophoretic microfluidic lab-chip that can precisely pair cells and achieve cell fusion with high efficiency. The chip demonstrates high cell fusion yields and maintains cell viability, making it a promising tool for cell fusion research.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Revathy Deivasigamani, Nur Nasyifa Mohd Maidin, M. F. Mohd Razip Wee, Mohd Ambri Mohamed, Muhamad Ramdzan Buyong
Summary: The study focuses on validating the behavior of polystyrene particles to predict their effects on keratinocytes, aiming to enhance wound healing applications.
Article
Biochemical Research Methods
Kazuma Yoda, Yoshiyasu Ichikawa, Masahiro Motosuke
Summary: This paper presents a newly developed high-throughput measurement device for determining the dielectric properties of cancer cells. The device utilizes continuous-flow electrorotation to measure the rotational behavior of cells, resulting in a significant improvement in throughput compared to conventional devices.
Article
Physics, Applied
Xiaoqian Zhu, Yuhang Li, Yuanxiang Zhou, Yunxiao Zhang, Xuanhao Liu, Ming Chen
Summary: Introducing a voltage stabilizer with high electron affinity can improve the electrical treeing resistance of silicone rubber and allow for adaptive migration and repositioning during the growth of electrical trees.
APPLIED PHYSICS LETTERS
(2023)
Article
Engineering, Biomedical
Liang Huang, Qiang Fang
Summary: This study presents a microfluidic chip that combines dielectrophoretic motion and electro-rotation technology for single-cell electrical properties characterization. The electrical properties of yeast cells were successfully characterized by analyzing the movement trajectory and rotation speed. This measurement method avoids the time-consuming traditional rotational spectra method, and achieves rapid and efficient single-cell electrical characterization.
BIOMEDICAL MICRODEVICES
(2021)
Article
Biochemical Research Methods
Nitipong Panklang, Kitipob Vijitnukoonpradit, Chaturong Putaporntip, Kesinee Chotivanich, Michihiko Nakano, Mati Horprathum, Boonchai Techaumnat
Summary: In this study, the dielectrophoresis (DEP) technique was used to characterize red blood cells and differentiate between normal and malaria-infected cells. The results showed significant differences in the crossover frequencies and cell velocity under negative dielectrophoresis between the infected and normal cells. The findings suggest that malaria infection affects the capacitance and conductance of the cell membrane.
Review
Chemistry, Analytical
Nur Shahira Abdul Nasir, Revathy Deivasigamani, M. F. Mohd Razip Wee, Azrul Azlan Hamzah, Mohd Hazani Mat Zaid, Muhammad Khairulanwar Abdul Rahim, Aminuddin Ahmad Kayani, Abdullah Abdulhameed, Muhamad Ramdzan Buyong
Summary: This article reviews three methods of electrical quantification of DEP responses and discusses their correlation with DEP responses. The study found that capacitance measurement is a reliable method for identifying DEP responses. Furthermore, the possibility of manipulating proteins and electrically quantifying DEP responses without the usage of dye or fluorescent probes is discussed.
Article
Biochemical Research Methods
Capucine Martin, Jonathan D. Johnston, Erin A. Henslee, Daan R. van der Veen, Fatima H. Labeed
Summary: Research reveals that the electrical properties in adipocytes play a crucial role in peripheral clock regulation, independent of transcription-translation feedback loops. Using dielectrophoresis, significant electrophysiological rhythms were observed in pre-adipocytes, with circadian (around 24 hours) cycles in membrane capacitance and radius properties, and ultradian (around 14 hours) rhythms in membrane conductance.
Article
Engineering, Biomedical
Yuwen Li, Can Huang, Song- Han, Arum Han
Summary: This paper presents a method for measuring the dielectric properties of cells, especially lipid-producing microalgae, at high frequency and single-cell resolution. By integrating electrorotation and negative dielectrophoresis methods, the dielectric properties of cells, including membrane capacitance and cytoplasm conductivity, can be obtained. This method can be used in various applications, such as screening microalgae, separating cells with different dielectric properties, identifying different cell types, and conducting basic biophysical analyses.
BIOMEDICAL MICRODEVICES
(2022)
Article
Chemistry, Multidisciplinary
Felix Pfisterer, Neus Godino, Tobias Gerling, Michael Kirschbaum
Summary: In this study, a microfluidic flow protocol for selective electroporation of predefined target cells identified in real-time by high-quality microscopic image analysis was presented. This method allows selective poration of target cells while non-target cells remain unaffected in heterogeneous cell samples.
Article
Multidisciplinary Sciences
Maliha Saleem Bakhshi, Mohsin Rizwan, Ghulam Jilany Khan, Hong Duan, Kefeng Zhai
Summary: In this study, a novel two-stage, label-free, rapid, and continuous CTC separation device based on hydrodynamic inertial focusing and dielectrophoretic separation is proposed. The device can effectively separate CTCs from other blood cells, yielding viable CTCs with high efficiency.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Carlos Honrado, Nadine Michel, John H. Moore, Armita Salahi, Veronica Porterfield, Michael J. McConnell, Nathan S. Swami
Summary: The translation of human-induced pluripotent stem cells into human neural progenitor cells has potential applications in drug discovery and transplant therapy for neurological diseases. By using electrophysiology for quantification of hNPC subpopulations, it is possible to control cell composition for optimal organoid development. This label-free detection strategy allows for faster optimization of cellular compositions and prevents loss of cell viability.
Article
Chemistry, Analytical
Armita Salahi, Carlos Honrado, Aditya Rane, Federica Caselli, Nathan S. Swami
Summary: This study demonstrates the use of red blood cells (RBCs) as multimodal standard particles to sensitively identify cells and compute biophysical information based on modified subcellular electrophysiology and associated fluorescence levels.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yi Liu, John H. Moore, Svetlana Harbaugh, Jorge Chavez, Chia-Fu Chou, Nathan S. Swami
Summary: Synthetic biology approaches have emerged as sensing paradigms for environmental and in vivo monitoring. To aid in the design and optimization of bacterial constructs for sensing analytes, there is a need for lysis-free intracellular detection modalities. This study presents an electrochemical detection system that allows for multiplexed assessment of expressed intracellular factors from different bacterial constructs.
Article
Biochemical Research Methods
Xuhai Huang, Karina Torres-Castro, Walter Varhue, Aditya Rane, Ahmed Rasin, Nathan S. Swami
Summary: In this study, a microfluidic strategy on a chip is proposed to transfer cells into media tailored for dielectrophoresis. The strategy utilizes tangential flows of low media conductivity to transfer cells from physiological media into a lower conductivity media, maintaining laminarity of the flow-focused sample and minimizing cell dispersion across streamlines.
Article
Chemistry, Analytical
Naimesh Sardesai, Yi Liu, Regina Shia, Peter Mirau, Jorge L. Chavez, Nathan S. Swami
Summary: This study explores the use of magnetic nanoparticles to amplify signals and alter the charge configuration of aptamers, enabling wash-free multiplexed detection of neurochemical biomarkers in serum media. A microchip platform with immobilized selective aptamer receptors is developed for the detection of cortisol and neuropeptide Y in patient-derived serum samples, validated by immunoassays. This sensor has the potential to profile a wider range of human performance biomarkers under stress-related events and develop stress augmentation methodologies.
Article
Biophysics
Armita Salahi, Aditya Rane, Li Xiao, Carlos Honrado, Xudong Li, Li Jin, Nathan S. Swami
Summary: Measurement and modulation of macrophage activation is crucial for addressing chronic back pain and immune rejection. Single-cell methods are needed to quantify the heterogeneity of macrophage activation phenotypes. This study presents a high throughput single-cell impedance cytometry for accurately measuring macrophage activation in a dose and duration dependent manner, without relying on specific signaling pathways.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Engineering, Biomedical
Mary Clare McCorry, Kenneth F. Reardon, Marcie Black, Chrysanthi Williams, Greta Babakhanova, Jeffrey M. Halpern, Sumona Sarkar, Nathan S. Swami, Katherine A. Mirica, Sarah Boermeester, Abbie Underhill
Summary: The use of engineered cells, tissues, and organs has the potential to revolutionize the treatment of injuries and diseases. However, the commercialization of these technologies is hindered by the complexity and cost of manufacturing. Real-time sensor technologies are crucial for ensuring consistent product quality and compatibility with manufacturing equipment.
Article
Materials Science, Multidisciplinary
Karina Torres-Castro, Javad Jarmoshti, Li Xiao, Aditya Rane, Armita Salahi, Li Jin, Xudong Li, Federica Caselli, Carlos Honrado, Nathan S. Swami
Summary: The integration of on-chip biophysical cytometry downstream of microfluidic enrichment allows for inline monitoring of phenotypic and separation metrics at single-cell sensitivity, enabling active control of separation and versatile application to different sample sets.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Biophysics
Armita Salahi, Carlos Honrado, John Moore, Sara Adair, Todd W. Bauer, Nathan S. Swami
Summary: Chemotherapy failure in pancreatic cancer patients is often caused by cancer cell reprogramming towards drug resistance by cancer associated fibroblasts (CAFs), which are abundant in the tumor microenvironment. Distinguishing drug resistant cancer cells from CAFs is challenging due to non-specific staining. Biophysical metrics from single-cell impedance cytometry are utilized to distinguish viable cancer cells from CAFs before and after gemcitabine treatment. Machine learning is used to train a classifier model, which can recognize and predict the proportions of each cell type in multicellular tumor samples, validated by confusion matrix and flow cytometry assays. The distinguishing biophysical metrics of viable drug resistant cancer cells in cocultures with CAFs can be used to identify markers in longitudinal studies.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Josie L. Duncan, Mathew Bloomfield, Nathan Swami, Daniela Cimini, Rafael V. Davalos
Summary: Aneuploidy is common in cancer, and tetraploidy often occurs during the evolution of aneuploid tumors. High-frequency DEP can distinguish cell subpopulations based on their ploidy and nuclear size, providing insight into their roles in cancer progression.
Article
Chemistry, Medicinal
John H. Moore, Armita Salahi, Carlos Honrado, Christopher Warburton, Steven Tate, Cirle A. Warren, Nathan S. Swami
Summary: Antibiotic-induced disruption of the microbiota creates conditions for colonization by opportunistic pathogens such as Clostridioides difficile, which causes severe hospital-acquired intestinal infections. A new ex vivo assay using C. difficile spore germination in fecal supernatant can predict intestinal dysbiosis and susceptibility to infection, providing a potential tool for assessing the impact of antibiotics on the host microbiota.
ACS INFECTIOUS DISEASES
(2023)
Article
Biochemical Research Methods
Carlos Honrado, Armita Salahi, Sara J. Adair, John H. Moore, Todd W. Bauer, Nathan S. Swami
Summary: Modulating cell death is crucial for treating pancreatic cancer, but distinguishing apoptotic states is challenging. In this study, machine learning methods were used to identify biophysical metrics that can differentiate early apoptotic, late apoptotic, and necrotic states, based on single-cell impedance cytometry. This automated apoptotic state classification provides insights for developing drug treatments.
Article
Chemistry, Analytical
Karina Torres-Castro, Mohammad S. Azimi, Walter B. Varhue, Carlos Honrado, Shayn M. Peirce, Nathan S. Swami
Summary: Islet transplantation is an expensive treatment for type 1 diabetes due to limited pancreas donors and variability in islet quality. Co-culturing islets with stem cells can promote remodeling and angiogenesis, but there is a need for metrics to assess islet reorganization. Biomechanical metrics based on aggregate shape evolution and microfluidic constriction pressures can monitor reorganization dynamics over time.
Article
Biochemical Research Methods
XuHai Huang, Karina Torres-Castro, Walter Varhue, Armita Salahi, Ahmed Rasin, Carlos Honrado, Audrey Brown, Jennifer Guler, Nathan S. Swami
Summary: This study introduces a novel method for cell separation using dielectrophoresis, which achieves efficient and precise separation by designing specific device geometries. Experimental validation on healthy and fixed red blood cells demonstrates the effectiveness of this method, highlighting its potential for further applications.
Review
Biochemical Research Methods
Carlos Honrado, Paolo Bisegna, Nathan S. Swami, Federica Caselli
Summary: Microfluidic impedance cytometry is a label-free and high-throughput method for stratifying cellular systems based on electrophysiology, with applications ranging from life science research to precision medicine. Novel chip designs and data analytic strategies are laying the foundation for multiparametric cell characterization and subpopulation distinction, essential for understanding biological function and disease progression. Emerging developments in the technique, such as device design, data analytics, and phenotyping applications, offer exciting prospects for future challenges and directions in label-free quantification and isolation of subpopulations in heterogeneous biosystems.