Journal
ELECTROPHORESIS
Volume 35, Issue 14, Pages 2029-2038Publisher
WILEY-BLACKWELL
DOI: 10.1002/elps.201400009
Keywords
Cardiac troponin I; Cationic isotachophoresis; Microfluidics; Preconcentration; Proteins
Funding
- National Institutes of Health [HL80186, HL80186-5S1]
- Life Science Discovery Fund
- Washington State University National Institutes of Health Protein Biotechnology Training Program from the NIGMS [T32GM008336]
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Cationic ITP was used to separate and concentrate fluorescently tagged cardiac troponin I (cTnI) from two proteins with similar isoelectric properties in a PMMA straight-channel microfluidic chip. In an initial set of experiments, cTnI was effectively separated from R-Phycoerythrin using cationic ITP in a pH 8 buffer system. Then, a second set of experiments was conducted in which cTnI was separated from a serum contaminant, albumin. Each experiment took similar to 10 min or less at low electric field strengths (34 V/cm) and demonstrated that cationic ITP could be used as an on-chip removal technique to isolate cTnI from albumin. In addition to the experimental work, a 1D numerical simulation of our cationic ITP experiments has been included to qualitatively validate experimental observations.
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