Article
Biodiversity Conservation
Marianna Fenzi, Jean Foyer, Valerie Boisvert, Hugo Perales
Summary: The problem of genetically modified maize contamination in Mexico is the result of planned and unplanned consequences of scientific and political choices. A risk management strategy based on the modernist dichotomy between modern and native has failed to protect Mexican landraces and marginalized other forms of knowledge. Farmers' seed systems are crucial for crop resilience and evolution.
PLANTS PEOPLE PLANET
(2023)
Article
Biotechnology & Applied Microbiology
Carlos Roman Collazo, Karen Chacha Guerrero, Tatiana Loja Mejia, Diego Andrade Campoverde, Yenima Hernandez Rodriguez
Summary: Partial approval of GMO use is research-oriented, with a rejection toward food. The linear regression model explained 65% of the variance of attitude toward GMOs from the beliefs, practices, knowledge, and bioethical approach variables. The sociodemographic variables were completely excluded from the model due to the absence of statistical significance.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Agronomy
Jennifer A. Anderson, James Mickelson, Brandon J. Fast, Nathan Smith, Robert C. Pauli, Carl Walker
Summary: DP915635 maize is genetically modified to control corn rootworm (Diabrotica spp.) by expressing the IPD079Ea protein, as well as to tolerate glufosinate herbicide by expressing the phosphinothricin acetyltransferase (PAT) protein. A field study in the United States and Canada showed that although there were statistically significant differences in early stand count and days to flowering compared to the control maize, these differences were not significant after adjustment of p-values. Composition analysis revealed statistically significant differences in 7 out of 79 analytes, but these differences were also not significant after adjustment. However, all composition values fell within the range of natural variation. These findings indicate that DP915635 maize is comparable to non-GM maize in terms of agronomic traits and composition.
GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN
(2023)
Article
Chemistry, Multidisciplinary
Yujun Xing, Jie Liang, Fei Dong, Jirong Wu, Jianrong Shi, Jianhong Xu, Jinke Wang
Summary: We developed a novel loop-mediated isothermal amplification (LAMP) method using DNA captured on polyacrylamide microparticles (PAMMPs) as templates (PAMMPs@DNA-LAMP) for rapid qualitative detection of genetically modified organisms (GMOs). The PAMMPs@DNA-LAMP method allowed for rapid DNA extraction (5-10 min) and amplification (around 30 min) at a constant temperature of 63°C. The DNA captured by PAMMPs could be effectively detected by both conventional and quantitative PCR (qPCR) and LAMP.
Article
Chemistry, Multidisciplinary
Xinyu Zhou, Shengjie Ge, Yue Sun, Menglin Ran, Yifan Liu, Yu Mao, Xiaowei Cao
Summary: A novel biosensor was developed for the analysis of target DNA in maize, utilizing a high-density hot spot SERS substrate and HCR amplification strategy, exhibiting significant sensitivity and a broad detection range. Additionally, SERS was applied to detect target DNA in maize extract, confirming the feasibility of the detection strategy.
NEW JOURNAL OF CHEMISTRY
(2021)
Review
Biotechnology & Applied Microbiology
Matan Arbel-Groissman, Itamar Menuhin-Gruman, Doron Naki, Shaked Bergman, Tamir Tuller
Summary: Evolutionary stability of synthetic constructs made of heterologous genes is a major challenge in synthetic biology, and the available tools to address this challenge are discussed in this review.
TRENDS IN BIOTECHNOLOGY
(2023)
Review
Agronomy
Alberto Santillan-Fernandez, Yolanda Salinas-Moreno, Jose Rene Valdez-Lazalde, Santiago Pereira-Lorenzo
Summary: This study analyzed the spatial-temporal evolution of scientific production on GMO maize, finding a linear growth in Latin American literature and low impact in scientific studies published in their own countries in Spanish. In Mexico, spatial discrepancies were found between research centers and production areas, limiting technology transfer, with a lack of specialized authors in GMO maize themes. Researchers responded to scientific trends in accordance with agrarian policies of the time.
Article
Agronomy
Yichun Hu, Liqin Linghu, Min Li, Deqian Mao, Yu Zhang, Xiaoguang Yang, Lichen Yang
Summary: This study analyzed and evaluated the nutritional components and protein quality of genetically modified maize expressing phytase gene. The results showed that the protein quality of the genetically modified maize was slightly higher than its parental maize and commercial available maize. Lysine was identified as the limiting amino acid for the genetically modified maize.
GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN
(2022)
Review
Biochemistry & Molecular Biology
Tongyun Luo, Lujing Li, Shirui Wang, Nan Cheng
Summary: This article provides an overview of genetically modified maize, including its varieties, applications, and relevant laws and regulations. It focuses on the existing methods for detecting nucleic acid levels in genetically modified maize, as well as traditional detection techniques and new technologies, particularly those involving sensors. The article also discusses the current limitations and challenges of GM maize nucleic acid testing and suggests future directions for the field.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biology
Pablo Rozas, Eduardo Kessi-Perez, Claudio Martinez
Summary: Genetic modification of living organisms has played a significant role in the research and development of various applications. However, regulatory frameworks have struggled to keep pace with new technologies and safety concerns. The emergence of new genome editing techniques has further challenged these frameworks. This review discusses the definitions of genetically modified organisms, the evolving genome editing tools, and how regulatory frameworks have responded to these technologies, with a focus on agricultural crops. It also explores the use of genetically modified organisms in the food industry, biomedical applications, and climate change solutions.
BIOLOGICAL RESEARCH
(2022)
Review
Pharmacology & Pharmacy
Vasavi Basarkar, Sharayu Govardhane, Pravin Shende
Summary: Genetically modified micro-organisms have shown better efficacy and targeted delivery in healthcare, particularly in the treatment of cancer, obesity, and HIV infection. Modifying micro-organisms and integrating different therapeutic fragments have enhanced their therapeutic activity. Genetically modified micro-organisms also have diverse applications in various sectors, such as electricity generation and water purification.
CURRENT PHARMACEUTICAL DESIGN
(2022)
Article
Plant Sciences
Ji-Eun Sim, Sung-Dug Oh, Kiyoon Kang, Yu-Mi Shin, Doh-Won Yun, So-Hyeon Baek, Yong-Eui Choi, Sang-Un Park, Jae-Kwang Kim
Summary: Using GC-MS-based metabolomics, we found that genetically modified rice had different metabolic profiles compared to non-genetically modified rice, potentially due to competition for the same precursor molecules. Additionally, metabolic composition in rice leaves was found to vary by growth stage rather than genetic modifications. These results demonstrate the potential of metabolomics for identifying metabolic alterations in response to genetic modifications.
Article
Chemistry, Applied
Jun Li, Hongfei Gao, Yunjing Li, Fang Xiao, Shanshan Zhai, Gang Wu, Yuhua Wu
Summary: A specific event-specific PCR method was established for the identification and quantification of GM maize DBN9936, showing that duplex ddPCR outperformed qPCR in terms of precision across different platforms.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Analytical
Qian Zhang, Weiran Wang, Zhansen Yang, Xin Wang, Wentao Xu, Kunlun Huang, Yunbo Luo, Xiaoyun He, Nan Cheng
Summary: A portable 3D-printed biosensing device was developed for rapid detection of genetically modified maize MON810, utilizing a rapid method for genomic DNA extraction and event-specific RPA amplification and LFS detection, with a qualitative detection limit of 0.1 wt%. The device proved to be sensitive and reliable with potential applications in rapid screening of risk factors for food safety.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Plant Sciences
Juliana Erika de Carvalho Teixeira Yassitepe, Viviane Cristina Heinzen da Silva, Jose Hernandes-Lopes, Ricardo Augusto Dante, Isabel Rodrigues Gerhardt, Fernanda Rausch Fernandes, Priscila Alves da Silva, Leticia Rios Vieira, Vanessa Bonatti, Paulo Arruda
Summary: Advancements in plant biotechnology have led to the development of genetically modified maize varieties that have had a significant impact on global agricultural management and grain yield improvement. Currently, genetically modified maize varieties cover a substantial portion of the maize cultivated area worldwide. The development of genetically modified maize is no longer limited by transformation technology, with emerging technologies pointing towards significant progress in maize biotechnology.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Analytical
David Moreno-Gonzalez, Delia Castilla-Fernandez, Pascal Vogel, Guanghui Niu, Sebastian Brandt, Carolin Drees, Juan F. Garcia-Reyes, Antonio Molina-Diaz, Joachim Franzke
Summary: This study evaluated the potential of a new plasma-based soft ionization source named CA-F mu TP for the determination of BTEX group in olive oil, showing advantages over EI with easier identification of BTEX mass spectrum. The sensitivity of CA-FmTP was comparable to EI, demonstrating potential usefulness for BTEX analysis in fatty complex matrixes like olive oil.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Delia Castilla-Fernandez, David Moreno-Gonzalez, Juan F. Garcia-Reyes, Evaristo Ballesteros, Antonio Molina-Diaz
Summary: Datura species are known for their high concentration of tropane alkaloids, which can lead to poisoning when accidentally mixed with edible crops. Testing on frozen spinach and spinach-based infant food products found that some samples had levels close to the maximum limits set by the European Union. This highlights the global concern over the presence of these compounds in various commodities.
Article
Chemistry, Analytical
Delia Castilla-Fernandez, David Moreno-Gonzalez, Ma Carmen Murillo-Cruz, Juan F. Garcia-Reyes, Antonio Molina-Diaz
Summary: The study evaluated three different clean-up strategies combined with QuEChERS extraction for determining fipronil and its metabolites in chicken eggs, with EMR-Lipid showing the best results.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Applied
Delia Castilla-Fernandez, David Moreno-Gonzalez, Bienvenida Gilbert-Lopez, Juan F. Garcia-Reyes, Antonio Molina-Diaz
Summary: Despite efforts to address pesticide residues in citrus-flavored soft drinks over more than ten years, a new study shows that the issue persists. Globally, 98% of samples were found to contain at least one pesticide, with 85% having more than 4 pesticides. Europe had the highest total amount of pesticides, likely due to higher juice concentrate content in samples.
Article
Food Science & Technology
Delia Castilla-Fernandez, David Moreno-Gonzalez, Marcos Bouza, Andrea Saez-Gomez, Evaristo Ballesteros, Juan F. Garcia-Reyes, Antonio Molina-Diaz
Summary: A novel sample treatment approach based on a modified QuEChERS method was developed for simultaneous determination of veterinary drug and pesticide residues in salmon. Enhanced Matrix Removal-Lipid dSPE cleanup sorbent was used to improve the performance of QuEChERS, showing significant enhancement in sample cleanup efficiency.
Article
Chemistry, Analytical
Marcos Bouza, Julio Garcia-Martinez, Bienvenida Gilbert-Lopez, David Moreno-Gonzalez, Priscilla Rocio-Bautista, David Parras-Guijarro, Alberto Sanchez-Vizcaino, Sebastian Brandt, Juan F. Garcia-Reyes, Antonio Molina-Diaz, Joachim Franzke
Summary: Dielectric barrier discharge ionization is a versatile technique for ionizing a wide range of molecules, including neutral lipids. This study evaluated the use of liquid chromatography coupled with dielectric barrier discharge ionization mass spectrometry for neutral lipid analysis. The results showed that this technique can provide improved sensitivity and comparable or better quantitation limits compared to atmospheric pressure chemical ionization. The analysis of archaeological samples revealed the potential vegetal origin of the stored material based on the presence of unsaturation and sterols.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Lucia Bartella, Marcos Bouza, Priscilla Rocio-Bautista, Leonardo Di Donna, Juan F. Garcia-Reyes, Antonio Molina-Diaz
Summary: Ambient mass spectrometry is an alternative to classical mass spectrometry that allows fast, in-situ, and high-throughput analysis of samples with minimal sample pretreatment. This study explores the capability of different ambient ionization sources to analyze raw wine for quality control purposes. The spectra obtained from these sources showed the ability to classify and differentiate wine samples based on various compounds, including organic acids, phenolic acids, derivatives, and low-molecular-mass flavonoids. The plasma-based methods offer the advantage of solventless analysis, while the paper spray (PS) approach provides a richer MS profile.
MICROCHEMICAL JOURNAL
(2022)
Article
Food Science & Technology
Delia Castilla-Fernandez, Priscilla Rocio-Bautista, David Moreno-Gonzalez, Juan F. Garcia-Reyes, Antonio Molina-Diaz
Summary: This study examined the extraction of mycotoxins from walnuts using solid-liquid extraction (SLE) and explored various clean-up methods. The main limitation in analyzing mycotoxins in fatty matrices was the strong matrix effect encountered. However, dilution and injection proved to be effective in reducing the matrix effect, and a viable dilute-and-shoot method was proposed.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Marcos Bouza, Julio Garcia-Martinez, Bienvenida Gilbert-Lopez, Sebastian Brandt, Juan F. Garcia-Reyes, Antonio Molina-Diaz, Joachim Franzke
Summary: Dielectric barrier discharge ionization (DBDI) is a versatile tool for small-molecule mass spectrometry applications, covering a wide range of molecules from polar to low polar. However, the complex plasma gas-phase interactions in DBDI have not been well studied. In this study, the ionization mechanisms of a two-ring DBDI ion source were evaluated using different discharge gases for vaporized liquid samples. The results showed that APCI and DBDI exhibited different ionization trends during PAH analysis, with APCI mainly producing [M + H]+ ions and DBDI showing the prevalence of charge exchange reactions. The addition of dopants in the gas phase shifted the ionization mechanisms towards charge exchange reactions and facilitated the formation of [M]??+ ion species.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Atalanti Christou, Ana B. Martinez-Piernas, Ioannis J. Stavrou, Juan F. Garcia-Reyes, Constantina P. Kapnissi-Christodoulou
Summary: The effects of ripening stage, processing method, and geographical origin on the chemical composition and properties of Carob were investigated in this study using HPLC-ESI-HRMS and chemometric analysis. The study found that Carob seeds had higher levels of polyphenols, and unripe pods were richer in polyphenols. Carob processing, particularly thermal treatment, also led to noticeable changes in its chemical composition. The geographical origin also had an impact on the phenolic composition of Carob.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Review
Chemistry, Analytical
Priscilla Rocio-Bautista, David Moreno-Gonzalez, Ana B. Martinez-Piernas, Juan F. Garcia-Reyes, Antonio Molina-Diaz
Summary: This review discusses the application of liquid chromatography-mass spectrometry (LC-MS) technique in the determination of glyphosate (Gly) and related compounds. The sample preparation methods and novel liquid chromatography/mass spectrometry approaches are introduced, and the analytical challenges, strengths, and drawbacks are critically discussed.
TRENDS IN ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Marcos Bouza, Norman Ahlmann, Juan F. Garcia-Reyes, Joachim Franzke
Summary: The present study investigated the potential of SALD-FμTP-MS as a rapid analytical technique for direct analysis of surface-deposited samples. Paper was used as the substrate and an infrared hand-held laser was employed for sample desorption. The optimization process involved the use of viscous solvents to enhance desorption and ionization efficiencies. Ethylene glycol (EG) was identified as a suitable solvent, improving sensitivity and substrate stability. The applicability of SALD-FμTP-MS was demonstrated by successfully analyzing food, water, and biological samples, particularly for thermolabile and polarity diverse compounds.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Andres J. Rascon, Priscilla Rocio-Bautista, David Moreno-Gonzalez, Juan F. Garcia-Reyes, Evaristo Ballesteros
Summary: The metal-organic framework CIM-80(Al) was proposed as a sorbent coating for solid-phase microextraction fibers. The CIM-80(Al)-based microextraction fibers with a thickness of 3.5 μm were used to isolate and determine 14 phthalate esters in bottled water with promising results. The extraction efficiency was comparable to commercial coatings in a total of 16 bottled water samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Education, Scientific Disciplines
Marcos Bouza, Bienvenida Gilbert-Lopez, Juan Francisco Garcia-Reyes, Pilar Gema Rodriguez Ortega
Summary: High-resolution mass spectrometry (HRMS) is becoming more affordable and user-friendly, with a wide range of applications. The experiment demonstrates the powerful and useful nature of HRMS in addressing research projects and chemistry problems, providing opportunities for discussions related to measurement precision and accuracy in an analytical chemistry context.
CHEMISTRY TEACHER INTERNATIONAL
(2022)
Review
Chemistry, Analytical
Miriam Beneito-Cambra, Bienvenida Gilbert-Lopez, David Moreno-Gonzalez, Marcos Bouza, Joachim Franzke, Juan F. Garcia-Reyes, Antonio Molina-Diaz
ANALYTICAL METHODS
(2020)
