Journal
ELECTROPHORESIS
Volume 33, Issue 15, Pages 2253-2265Publisher
WILEY-BLACKWELL
DOI: 10.1002/elps.201200050
Keywords
Aflatoxin; Capillary electromigration; Lateral flow; Microfluidic immunosensor; Mycotoxin
Funding
- National Natural Science Foundation of China [31171702, 31101299]
- Natural Science Foundation of Hubei Province [2011CDB358, 2011CDB356]
- Key Project of the Ministry of Agriculture [2010-G1, 2011-G5]
- Project of National Science & Technology Pillar Plan [2012BAB19B09, 2012BAK08B03]
- Special Fund for Agro-scientific Research in the Public Interest [201203094, 201203070, 201203037, 2012ZL042]
- Earmarked Fund for China Agriculture Research System [CARS-13]
- Special Foundation of President of the Chinese Agricultural Academy of Sciences [1610172010003]
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Mycotoxin contamination in the food chain has caused serious health issues in humans and animals. Thus, a rapid on-site and lab-independent detection method for mycotoxins, such as aflatoxins (AFTs), is desirable. Microfluidic chip based immunosensor technology is one of the most promising methods for fast mycotoxin assays. In this review, we cover the major microfluidic immunosensors used for mycotoxin analysis, via flow-through (capillary electromigration) and lateral flow technology. Sample preparation from different matrices of agricultural products and foodstuffs is summarized. The choice of materials, fabrication strategies, and detection methods for microfluidic immunosensors are further discussed in detail. The sensors application in mycotoxin determination is also outlined. Finally, future challenges and opportunities are discussed.
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