Article
Chemistry, Analytical
Somayyeh Bakhtiaridoost, Hamidreza Habibiyan, Hassan Ghafoorifard
Summary: This study presents a fast, hemolysis-free, highly efficient blood plasma separation microfluidic device that combines gravitational sedimentation with dielectrophoresis force. Using finite element analysis, the effect of geometrical parameters on the separation process is investigated to achieve optimal design specifications. The device achieves a purity of 99.98% and a relative increase in flow rate compared to previous studies by utilizing a porous Melt-Blown Polypropylene layer and optimizing the dielectrophoresis force.
ANALYTICA CHIMICA ACTA
(2023)
Article
Physics, Applied
Filippos Harlaftis, Dean Kos, Qianqi Lin, Kevin T. P. Lim, Calvin Dumesnil, Jeremy J. J. Baumberg
Summary: Dielectrophoresis is found to drive the motion of nanoparticles through the interaction of their induced dipoles with a non-uniform electric field. The reversible aggregation of 100 nm diameter gold nanoparticles in electrode gaps is experimentally observed under MHz frequencies. A frequency resonance is observed, resulting in a significant increase in the density of gold nanoparticle clouds. A simple model is proposed to explain this reversibility, suggesting the possibility of substantial control over nanoparticle separation.
APPLIED PHYSICS LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Luis G. Gonzalez-Arostegui, Alberto Munoz-Prieto, Asta Tvarijonaviciute, Jose Joaquin Ceron, Camila Peres Rubio
Summary: The study demonstrated that a comprehensive panel of redox biomarkers can be measured in the whole blood and red blood cells of dogs, with whole blood being more sensitive to changes in experimental conditions. This suggests that whole blood is a promising sample for evaluating the redox status in dogs.
Article
Chemistry, Analytical
Maria Dimaki, Mark Holm Olsen, Noemi Rozlosnik, Winnie E. Svendsen
Summary: This paper introduces a novel microfluidic chip that utilizes electrical forces generated by a DC or AC field to achieve upconcentration of sub-100 nm nanoparticles in a flow. Two optimized electrode designs were tested using particles as small as 47 nm, and inclined electrodes with a zig-zag three-tooth configuration in a 20 μm width channel were found to generate the highest gradient and force. The chip design based on AC dielectrophoresis successfully upconcentrated sub-100 nm particles by a factor of 11 using a flow rate of 2-25 μL/h. The paper presents theoretical and experimental results, and discusses the potential for massively parallelizing the chip design to significantly increase throughput.
Article
Biochemical Research Methods
Logeeshan Velmanickam, Vidura Jayasooriya, Dharmakeerthi Nawarathna
Summary: The study introduces a disposable universal point-of-care biosensor that can detect and quantify nucleic acid and proteomic biomarkers in diluted serum samples, offering fast, high sensitivity, and low-cost testing capabilities.
Article
Medicine, General & Internal
Vincent M. Tutino, Yongjun Lu, Daizo Ishii, Kerry E. Poppenberg, Hamidreza Rajabzadeh-Oghaz, Adnan H. Siddiqui, David M. Hasan
Summary: This study found expression differences in half of the IA-associated genes in the blood within the aneurysm, providing evidence for IA tissue-mediated transcriptional changes in whole blood. Additionally, some genes may be informative in assessing IA risk, as their intraluminal expression was correlated to IA size and aneurysmal wall enhancement.
Article
Multidisciplinary Sciences
Ananya Malhotra, Bernard Rachet, Audrey Bonaventure, Stephen P. Pereira, Laura M. Woods
Summary: This study made progress in early detection of high-risk pancreatic cancer patients using machine learning, predicting the likelihood of patients developing pancreatic cancer before diagnosis. By combining the algorithm with existing biomarker tests, more tumors could be diagnosed earlier, potentially increasing survival rates for patients.
Article
Chemistry, Analytical
Fleming Dackson Gudagunti, Srilakshmi G. Gundlakunta, Ivan T. Lima
Summary: This study demonstrates that dielectrophoresis (DEP) spectroscopy is an effective transduction mechanism for detecting the concentration levels of pancreatic cancer biomarkers CA 242 and CEA in serum. By observing the frequency dependence of the negative DEP force applied on functionalized polystyrene microspheres, the researchers were able to measure changes in the number of cancer antigens bound to the microspheres. The use of an electrode array with a well-defined electric field gradient enabled automated signal processing and reproducibility of the biosensor signal.
Article
Chemistry, Analytical
Yameng Liu, Mark A. Hayes
Summary: Proteins are complex and difficult to analyze, but dielectrophoresis can be used to characterize and concentrate them. Forces exerted on different proteins are larger than predicted by traditional theory, leading to more effective separation and concentration.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Olivia Ruhen, Bob Mirzai, Michael E. Clark, Bella Nguyen, Carlos Salomon, Wendy Erber, Katie Meehan
Summary: Increasing recognition of ctDNA as a non-invasive alternative for molecular characterisation and disease monitoring. Limited research on the relationship between EV DNA and ctDNA, with ctDNA showing greater sensitivity in monitoring breast cancer progression.
Article
Chemistry, Multidisciplinary
Taher Ghomian, Hyunhak Jeong, Victor Pan, Kubra Celik, Mashari Alangari, Yonggang Ke, Joshua Hihath
Summary: Accurate control over the location of nanostructured materials is crucial for studying electronic properties. A dielectrophoretic device with high throughput and low-cost manufacturing process has been developed, allowing controlled trapping of high-impedance nanomaterials. The device utilizes a scalable-electronic circuit to measure capacitance variation and successfully trap 1D DNA origamis demonstrating accurate positioning.
ADVANCED MATERIALS INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Shuailong Zhang, Weizhen Li, Mohamed Elsayed, Jiaxi Peng, Yujie Chen, Yanfeng Zhang, Yibo Zhang, Moein Shayegannia, Wenkun Dou, Tiancong Wang, Yu Sun, Nazir P. Kherani, Steven L. Neale, Aaron R. Wheeler
Summary: A method based on optoelectronic tweezers for forming topographical micropatterns (TMPs) has been reported, showing promise in constructing structures in the field of microelectronics. This technique demonstrates the ability to create permanent structures that can be harvested and transferred, paving the way for new applications in microelectronics.
Review
Biochemical Research Methods
Logeeshan Velmanickam, Vidura Jayasooriya, Madhava Sarma Vemuri, Umamaheswara Rao Tida, Dharmakeerthi Nawarathna
Summary: Dielectrophoresis is a widely utilized phenomenon in biomedical applications, particularly in the development of devices integrated with microfluidics, fluorescence, and electrical impedance for disease screening and diagnosis. Recent research has also shown the potential value of machine learning approaches in improving biomarker detection performance.
Article
Respiratory System
Mina Gaga, Joanna Chorostowska-Wynimko, Ildiko Horvath, Martin C. Tammemagi, David Shitrit, Vered H. Eisenberg, Hao Liang, David Stav, Dan Levy Faber, Maarten Jansen, Yael Raviv, Vasileios Panagoulias, Piotr Rudzinski, Gabriel Izbicki, Ohad Ronen, Adiv Goldhaber, Rawia Moalem, Nadir Arber, Ilana Haas, Qinghua Zhou
Summary: The study validated the performance of the methylation-based blood test Lung EpiCheck in the detection of lung cancer in European and Chinese samples, showing high detection rates for early-stage non-small cell lung cancer and small cell lung cancer.
EUROPEAN RESPIRATORY JOURNAL
(2021)
Article
Oncology
Yibo Gao, Hengqiang Zhao, Ke An, Zongzhi Liu, Luo Hai, Renda Li, Yang Zhou, Weipeng Zhao, Yongsheng Jia, Nan Wu, Lingyu Li, Jianming Ying, Jie Wang, Binghe Xu, Zhihong Wu, Zhongsheng Tong, Jie He, Yingli Sun
Summary: This study provides a toolset for generating unbiased whole-genome ctDNA methylomes with a minimal amount of plasma, enabling the development of highly specific and sensitive biomarkers for early cancer diagnosis and molecular subtyping.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)