Article
Reproductive Biology
Yuehuan Li, Taylor Elijah Martin, Jonathan Matthew Hancock, Rong Li, Suvitha Viswanathan, John P. Lydon, Yi Zheng, Xiaoqin Ye
Summary: Uterine fluid plays important roles in supporting early pregnancy events and its timely absorption is critical for embryo implantation. In mice, its volume is maximum on day 0.5 post-coitum (D0.5) and approaches minimum upon embryo attachment similar to D4.0. Its secretion and absorption in ovariectomized rodents were shown to be promoted by estrogen and progesterone (P4), respectively. The temporal mechanisms in preimplantation uterine fluid absorption remain to be elucidated.
BIOLOGY OF REPRODUCTION
(2023)
Article
Chemistry, Multidisciplinary
Pallab Basuri, Jenifer Shantha Kumar, Keerthana Unni, Sujan Manna, Thalappil Pradeep
Summary: Molecular de-aggregation at the air/water interface of aqueous microdroplets was observed, which is influenced by the rapid evaporation of the solvent.
CHEMICAL COMMUNICATIONS
(2022)
Article
Optics
Xuemiao Zhao, Lianyu Zhang, Xin Lv, Jinhua Liu, Xinxin Liu, Yuting Zhang, Dong Zhang, Shuhong Li, Qingru Wang
Summary: By embedding in-situ gold particles into poly (vinyl alcohol) nanocomposite films, the fluorescence of rhodamine 6G can be enhanced with a maximum enhancement factor of 13. The localized surface plasmon resonance bands of the substrate have a significant impact on the enhancement factor. The substrate can also attenuate fluorescence quenching. Furthermore, the mechanism of surface enhanced fluorescence is demonstrated by simulating the local electric field distribution of the substrate using FDTD method.
Article
Optics
Suhyeon Park, Anatoly Parahovnik, Yoav Peles, Subith S. Vasu
Summary: This work demonstrated a thermometric technique using LIF in supercritical carbon dioxide flows to measure temperatures at high pressures. The temperature-sensitive fluorescent dye used showed good performance, closely related to the temperatures obtained from sensors.
Article
Cell Biology
Jing-Jing Cui, Jia Wang, Dong-Sheng Xu, Shuang Wu, Ya-Ting Guo, Yu-Xin Su, Yi-Han Liu, Yu-Qing Wang, Xiang-Hong Jing, Wan-Zhu Bai
Summary: Neural tract tracing using AF488-CTB was effective in labeling motor, sensory, and sympathetic neurons with clear and complete morphology, but image quality deteriorated after 14 days post-injection.
NEURAL REGENERATION RESEARCH
(2022)
Article
Optics
Audrey Eshun, Xiyu Yi, Ashleigh Wilson, Sam Jeppson, Jae Hyuck Yoo, Shervin Kiannejad, Mike Rushford, Tiziana Bond, Ted Laurence
Summary: We utilized photon pair correlations generated via SPDC to measure the fluorescence lifetime of rhodamine 6 G dye. Our method, Q-FL measurements, used entangled photons to excite fluorescence and timed the resulting fluorescence photon in reference to the arrival time of the other entangled photon. This measurement demonstrates the potential of using entangled photons for fluorescence imaging with their fast sub-100 ps correlations that have not been shown with classical methods.
Article
Chemistry, Multidisciplinary
Sigurd Mertens, Bernhard Siegmund, Koen Vandewal
Summary: This study combines spectroscopic measurements with photothermal techniques to accurately determine the photoluminescence quantum yield (PLQY) of six fluorescent molecules in low concentration solutions. The measurement method demonstrates high reliability and can detect phonon-assisted optical upconversion when the photon energy is below the photothermal threshold. It is found that perylene red solution has the highest PLQY, while the presence of low energy sub-gap impurities in perylene orange solution prevents upconversion.
MATERIALS HORIZONS
(2023)
Article
Chemistry, Multidisciplinary
Inhong Kim, Juyeong Jang, Seunghwan Lee, Won-Geun Kim, Jin-Woo Oh, Irene Wang, Jean-Claude Vial, Kwangseuk Kyhm
Summary: The research measured the optical modal gain of a dye-virus hybrid structure using a variable stripe length method, showing strong optical anisotropy in edge emission and the appearance of a spectral etalon in the gain spectrum. These results were attributed to the corrugated structure and effective cavity lengths were estimated.
Article
Chemistry, Analytical
Lu Li, Xin Lyu, Saibo Liang, Zhanfang Liu
Summary: In this study, a probe was developed using Rhodamine 6G (Rh6G) within hollow SiO2 photonic crystals (HSPCs) to amplify the fluorescent signal for accurate and sensitive detection of hydrogen peroxide (H2O2) content. By incorporating I- into the system, I- can be oxidized to I2 in the presence of H2O2, and the produced I3- can react with Rh6G to form a complex that quenches the fluorescence signal, enabling highly efficient H2O2 detection. This method does not require complex organic synthesis or modification of fluorescent groups, and the sensors exhibit good performance in terms of repeated use and potential detection of different concentrations of ethanol solutions.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Inorganic & Nuclear
G. Cabello-Guzman, Mathias Seguel, Luis Fernandez, Claudia Caro, Cristian Suarez, Marcela Matus, Camila Cifuentes, Felipe Bustos, Katherine Ariz
Summary: We propose a synthesis strategy using β-triketonate complexes of Zn(II) and Cu(II) as precursors for the photo-deposition of CuO-loaded ZnO films. The resulting ZnO/CuO heterojunction system showed low crystallinity and the presence of both ZnO and CuO. The optical properties revealed a gradual reduction of the optical bandgap with increasing CuO loading, and photoluminescence tests showed decreased emission bands with the insertion of CuO. The ZnO films loaded with 1 mol% CuO exhibited high photo-degradation efficiency for rhodamine B and rhodamine 6G.
INORGANIC CHEMISTRY COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Serkan Erdemir, Sait Malkondu
Summary: A novel probe RHI was designed and synthesized for sensitive and specific sensing of metal ions, showing potential for biological and environmental applications.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Chemistry, Physical
Bryce P. Hickam, Manni He, Nathan Harper, Szilard Szoke, Scott K. Cushing
Summary: A spectrometer capable of characterizing the temporal and spectral properties of entangled photon states has been constructed and used to investigate the entangled two-photon interaction of rhodamine 6G molecule. The results reveal that the entangled two-photon absorption interaction is equal to or weaker than classical, single-photon scattering events, which explains the discrepancies reported from different measurement techniques.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Inorganic & Nuclear
Mani Rajasekar, Saravanan Geetha Sree Agash, Chiterasu Narendran, Kumarasan Rajasekar
Summary: Luminescent chemosensors for sensing Cu2+ have gained significant attention in recent years due to their potential applications in biochemistry and environmental research. These sensors are based on host-guest interactions, which include van der Waals forces, hydrophobic contacts, electrostatic forces, coordination between metal ligands, and hydrogen bonds. They allow for selective detection of different species, making them valuable for detecting metal ions in biological and environmental systems. Small-molecule fluorescence probes that utilize selective, bio-orthogonal chemistry have been summarized for their ability to recognize Cu2+ ions in this article.
INORGANIC CHEMISTRY COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Behnam Gheitarani, Marzieh Golshan, Seyedeh-Arefeh Safavi-Mirmahalleh, Mehdi Salami-Kalajahi, Mahdi Salami Hosseini, Ali Akbar Alizadeh
Summary: In this study, fluorescent monomers based on rhodamine 6G and coumarin were synthesized and copolymers were obtained through RAFT polymerization. The copolymers showed potential for selective detection of Fe3+.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Optics
Erik Busley, Leon Espert Miranda, Christian Kurtscheid, Frederik Wolf, Frank Vewinger, Julian Schmitt, Martin Weitz
Summary: The Liouville theorem states that the phase-space volume of an ensemble in a closed system remains constant. However, the nonconservation of photons upon contact with matter imposes fundamental limits for three-dimensional light-harvesting systems. In this work, we theoretically show phase-space compression of sunlight by cooling the captured radiation to room temperature.
Article
Chemistry, Analytical
Ashwin Ramachandran, Juan G. Santiago
Summary: The study examines the limits of detection and reaction times of CRISPR-diagnostic assays by developing a model based on enzyme kinetics theory. Experimental validation of the model using LbCas12a reaction kinetics data shows consistency with predicted scaling. The research highlights implications for achievable limits of detection and assay times in emerging CRISPR-detection methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Instruments & Instrumentation
Diego A. Huyke, Ashwin Ramachandran, Oscar Ramirez-Neri, Jose A. Guerrero-Cruz, Leland B. Gee, Augustin Braun, Dimosthenis Sokaras, Brenda Garcia-Estrada, Edward Solomon, Britt Hedman, Mario U. Delgado-Jaime, Daniel P. DePonte, Thomas Kroll, Juan G. Santiago
Summary: A three-dimensionally microfabricated microfluidic mixer platform compatible with time-resolved X-ray absorption and emission spectroscopy has been developed for determining electronic structures during chemical reactions. The platform allows for hydrodynamic focusing of the sample stream in 3D and enables prediction of residence time and mixing times, with a theoretical minimum mixing time of 0.91 ms.
JOURNAL OF SYNCHROTRON RADIATION
(2021)
Article
Chemistry, Analytical
Qi Jiang, Ashwin Ramachandran, Juan G. Santiago
Summary: The model presented quantifies the acceleration effects of ITP on chemical reactions, analyzing the impact of reaction rate constants and species abundance on product formation rate, using perturbation methods to study the effects of reverse reaction rate and relative species abundance on production rate.
ANALYTICAL CHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Robert D. Chambers, Nathanael C. Yoder, Aletha B. Carson, Christian Junge, David E. Allen, Laura M. Prescott, Sophie Bradley, Garrett Wymore, Kevin Lloyd, Scott Lyle
Summary: Collar-mounted activity monitors with battery-powered accelerometers accurately analyze canine behaviors and activity levels. Machine learning algorithms continually update to detect important health-related canine behaviors with high accuracy. The study's large and realistic training dataset, along with user validation, confirmed the accuracy of the algorithms in production.
Article
Chemistry, Analytical
Nobuyuki Futai, Yuto Fukazawa, Tomohiro Kashiwagi, Shogo Tamaki, Riho Sakai, Catherine A. Hogan, Kanagavel Murugesan, Ashwin Ramachandran, Niaz Banaei, Juan G. Santiago
Summary: This study presents a method for the high-efficiency separation and extraction of short cfDNA fragments, using a millimeter-scale fluidic device to extract the fragments into solid agarose gel slabs from liquid human plasma samples. The method demonstrates high sensitivity in cfDNA detection.
ANALYTICA CHIMICA ACTA
(2022)
Correction
Instruments & Instrumentation
Diego A. Huyke, Ashwin Ramachandran, Oscar Ramirez-Neri, Jose A. Guerrero-Cruz, Leland B. Gee, Augustin Braun, Dimosthenis Sokaras, Brenda Garcia-Estrada, Edward I. Solomon, Britt Hedman, Mario U. Delgado-Jaime, Daniel P. DePonte, Thomas Kroll, Juan G. Santiago
JOURNAL OF SYNCHROTRON RADIATION
(2022)
Article
Chemistry, Analytical
Diego A. Huyke, Ashwin Ramachandran, Vladimir Bashkirov, Efthalia K. Kotseroglou, Theofilos Kotseroglou, Juan G. Santiago
Summary: This study quantifies the kinetic parameters and detection limits of enzymes in CRISPR-Cas12 and CRISPR-Cas13 detection, and validates the self-consistency of the measurements. The results indicate that successful target detection requires the cleavage of at least 0.1% of the fluorescent reporter molecules, which is independent of the detection method and detector sensitivity.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Ashwin Ramachandran, Juan G. Santiago
Summary: Isotachophoresis (ITP) is a versatile electrophoretic technique used for sample processing and manipulation in microfluidic systems. A review of ITP theory and analysis can help advance its adoption and application in the scientific and engineering communities.
Review
Chemistry, Multidisciplinary
Mohammad A. Alkhadra, Xiao Su, Matthew E. Suss, Huanhuan Tian, Eric N. Guyes, Amit N. Shocron, Kameron M. Conforti, J. Pedro de Souza, Nayeong Kim, Michele Tedesco, Khoiruddin Khoiruddin, I. Gede Wenten, Juan G. Santiago, T. Alan Hatton, Martin Z. Bazant
Summary: This review provides a comprehensive description of the principles and applications of electrochemical methods for water purification, ion separations, and energy conversion. Electrochemical methods have attractive features such as compact size, chemical selectivity, broad applicability, and reduced generation of secondary waste. They directly remove contaminants from water, enabling efficient removal of trace pollutants.
Article
Chemistry, Analytical
Charles Blanluet, Diego A. Huyke, Ashwin Ramachandran, Alexandre S. Avaro, Juan G. Santiago
Summary: This study demonstrates that endpoint-based assays in CRISPR-Cas12 detection are limited by experimental choices and work best for known target concentrations. It also reveals that SNP activation results in measurable changes in the kinetic parameters of Cas12 trans-cleavage. To overcome the limitations of endpoint-based assays, the researchers developed a new assay based on the quantification of Michaelis-Menten parameters.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Alexandre S. Avaro, Juan G. Santiago
Summary: This article presents a quantification of the uncertainty in the experimental determination of kinetic rate parameters for enzymatic reactions. The authors examine several sources of uncertainty and bias and compute typical uncertainties of kcat, KM, and catalytic efficiency. The extraction of these parameters for CRISPR-Cas systems is analyzed as a salient example. Reports of enzymatic kinetic rates for CRISPR diagnostics have been highly unreliable and inconsistent.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
David J. Hoffman, Hans A. Bechtel, Diego A. Huyke, Juan G. Santiago, Daniel P. DePonte, Jake D. Koralek
Summary: This study demonstrates a method for generating multilayer thin film stacks of liquids, called liquid heterostructures. These free-flowing layered liquid sheets are produced with a microfluidic nozzle, resulting in the formation of two layers of one liquid enveloping an inner layer of another liquid. The buried liquid layer has a tunable thickness and well-defined liquid-liquid interfaces, making them ideal targets for spectroscopy and scattering experiments.
Review
Biochemical Research Methods
Alexandre S. Avaro, Juan G. Santiago
Summary: This review introduces nucleic acid detection assays that combine CRISPR-based diagnostics with microfluidic devices and techniques. It serves as a reference for researchers who want to use CRISPR-Cas systems for diagnostics in microfluidic devices. The review is organized into sections that cover the common workflow steps of CRISPR-based assays. The described systems include custom microfluidic chips, benchtop chip control devices, partition formats, and lateral flow biosensors. While CRISPR-based, microfluidics-driven assays offer specific detection and compatibility with combinatorial implementation, their low kinetic rate activity and reliance on off-chip assay steps are limitations.
Article
Mechanics
Diego A. A. Huyke, Alexandre S. S. Avaro, Thomas Kroll, Juan G. G. Santiago
Summary: Microfluidic mixers enable the study of fast reaction kinetics on the microsecond time scale, while soft X-ray absorption spectroscopy offers powerful analysis techniques. This study presents a novel micromixer and jet device that addresses the constraints of mixing time scales, sample volume, detection region size, and component materials. The system uses a 'theta' mixer composed of sintered and fused glass capillaries to rapidly accelerate and laminate the sample stream, inducing diffusive mixing within 0.9 microseconds. The micromixer allows for direct optical access to the mixing zone, enabling the analysis of reactions with fast kinetics using techniques such as soft X-ray spectroscopy.
Article
Biochemical Research Methods
Amit Jangra, Shaurya Shriyam, Juan G. Santiago, Supreet Singh Bahga
Summary: This article presents the development and demonstration of a neural network model for predicting whether a chosen analyte is focused by an isotachophoresis buffer system. The model was trained and tested using extensive data sets and achieved a prediction accuracy of over 97.7%. The applicability of the model was validated using experimental data, and a web-based application called IONN was developed for rapid screening of isotachophoresis electrolyte systems.