Article
Chemistry, Analytical
Craig Watson, Chao Liu, Ali Ansari, Helen C. Miranda, Rodrigo A. Somoza, Samuel E. Senyo
Summary: This article presents a microfluidic chip that allows the culture of cells in adjacent chambers and enables selective exchange of paracrine signals. The chip offers both high throughput and control over the onset and duration of cell-cell communication.
Article
Multidisciplinary Sciences
Sepideh Yazdian Kashani, Mostafa Keshavarz Moraveji, Shahin Bonakdar
Summary: This study demonstrated the successful development of an integrated microfluidic device based on cell imprinting for promoting directional differentiation of stem cells. Through validated numerical simulations and experimental evaluations, the effectiveness of the device in chondrocyte-induced differentiation was confirmed.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Nagham Khouri-Farah, Qiuxia Guo, Kerry Morgan, Jihye Shin, James Y. H. Li
Summary: Recent studies using single-cell RNA-sequencing have identified cellular heterogeneity in the developing mammalian cerebellum, but the regulatory logic behind this diversity remains unclear. Researchers integrated single-cell RNA and ATAC analyses to understand the developmental trajectories of cerebellar progenitors and identified potential regulatory elements controlling cell state transitions. Using gene regulatory network analysis, they uncovered the molecular control of a specific progenitor zone and demonstrated its association with a common cerebellar birth defect in humans.
Article
Multidisciplinary Sciences
Hao Yang, Mingjie Zhu, Tao Chen, Fuzhou Niu, Lining Sun, Liang Cheng
Summary: Cell mechanics is closely related to cellular functions and can serve as an effective biomarker for disease onset and progression. However, current techniques for measuring cell mechanical properties have limited data and biological significance. This study proposes a dielectrophoresis-based solution that combines a microfluidic device and an automatic control scheme to automatically obtain cell mechanical data. Experiments demonstrate the automation capability of this device.
Article
Materials Science, Biomaterials
Sepideh Yazdian Kashani, Mostafa Keshavarz Moraveji, Mojtaba Taghipoor, Reza Kowsari-Esfahan, Ali Akbar Hosseini, Leila Montazeri, Mohammad Mehdi Dehghan, Hossein Gholami, Saeed Farzad-Mohajeri, Morteza Mehrjoo, Mohammad Majidi, Philippe Renaud, Shahin Bonakdar
Summary: This study demonstrates the potential of the imprinting method in inducing chondrogenicity in stem cells, with successful regeneration of articular cartilage defects in rabbits after transplantation. The efficiency of the imprinting method was increased using microfluidic chip design and fabrication, leading to the precise placement of stem cells on chondrocyte patterns on the substrate.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Biochemical Research Methods
Feipeng Yang, Alanis Carmona, Katerina Stojkova, Eric Ivan Garcia Huitron, Anna Goddi, Abhinav Bhushan, Ronald N. Cohen, Eric M. Brey
Summary: A microfluidic system was developed to engineer a 3D human adipose microtissue, showing characteristics of adipose tissue and supported by interstitial flow. This system has the potential to serve as a platform for in vitro drug testing for adipose tissue-related diseases.
Article
Chemistry, Analytical
Esma Dervisevic, Muamer Dervisevic, Bryan Ang, James Carthew, Kellie L. Tuck, Nicolas H. Voelcker, Victor J. Cadarso
Summary: Microbial contamination in cell culture is a common problem with limitations in traditional detection methods. This article presents a new microfluidic device that can monitor cell culture media acidity and cellular glucose metabolism to detect bacterial contamination. Using this device, bacterial contamination can be detected at stages where traditional methods fail, reducing the risk of undetected contamination and increasing experimental reliability.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Yuqian Zhang, Yuguang Liu
Summary: A comprehensive system combining electrochemical impedance spectroscopy and digital microfluidics has been introduced to easily and sensitively evaluate a patient's immune function. The unique droplet manipulation feature of the microfluidic platform allows for dynamic cell capture assay, improving detection signal by 2.4-fold, demonstrating high sensitivity.
Article
Chemistry, Physical
U. S. Jayapiriya, Prakash Rewatkar, Sanket Goel
Summary: This study established a simple, customized, and economical laser-induced graphene (LIG) material using a CO2 laser, integrated into a microfluidic device for Enzymatic Biofuel Cell (EBFC) application. The modified C-LIG electrodes showed improved performance and enzyme stability, generating higher power density compared to LIG electrodes. The novel platform provides a simple and quick fabrication method with potential for further optimization and improvements in power output.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Cell Biology
Anny W. Robert, Marco A. Stimamiglio
Summary: This study evaluated the effects of conditioned medium (CM) collected at different time points during in vitro cardiomyogenesis and found that CM obtained at the initial time points could promote cell proliferation. However, the secretome derived from cardiac committed cells during cardiomyogenesis was unable to improve angiogenesis or migration in endothelial cells, or to stimulate the differentiation of cardioblasts.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Lingyan Gong, Chayakorn Petchakup, Pujiang Shi, Pei Leng Tan, Lay Poh Tan, Chor Yong Tay, Han Wei Hou
Summary: A novel microfluidic approach for label-free and continuous-flow monitoring of single spheroid/microcarrier based on electrical impedance spectroscopy is reported, with unique impedance signatures identified for optimal spheroid growth and viability monitoring. The platform offers great versatility for multi-parametric analysis of spheroids/microcarriers at high throughput, and can be integrated into bioreactors for long-term and remote monitoring of biomass and cell quality.
Article
Cell Biology
Michaela Mrugala Rothova, Alexander Valentin Nielsen, Martin Proks, Yan Fung Wong, Alba Redo Riveiro, Madeleine Linneberg-Agerholm, Eyal David, Ido Amit, Ala Trusina, Joshua Mark Brickman
Summary: Using single-cell transcriptomics, researchers have studied the different pathways of cell differentiation and identified an intermediate state in which cells transition from an extra-embryonic identity to an embryonic one. This finding has important implications for understanding organ differentiation in vitro.
NATURE CELL BIOLOGY
(2022)
Article
Engineering, Electrical & Electronic
Mate Kalovics, Kristof Ivan, Zsolt Szabo
Summary: A microfluidic mixer is integrated with a dual-band microwave resonator for in situ monitoring of liquid mixing. The mixer utilizes a complementary double split-ring resonator etched in the ground plane of a microstrip transmission line. Through simulations, optimal channel configurations are designed to increase sensitivity, minimize required sample volume, and eliminate unwanted microfluidic mixing. The device allows for unambiguous chemical identification by monitoring the frequency variation of resonances.
IEEE SENSORS JOURNAL
(2023)
Article
Multidisciplinary Sciences
Bingchen Che, Jie Zhu, Dan Sun, Xiaoqiang Feng, Ce Zhang
Summary: Simulating in vivo environmental conditions is crucial for in vitro studies on complex life machinery, and the design and fabrication of a microfluidic system can effectively demonstrate morphological and expression level changes of neural stem cells, providing a suitable platform for high throughput studies on complex life machinery.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Cell & Tissue Engineering
Jingran Zhang, Guang Shi, Junjie Pang, Xing Zhu, Qingcai Feng, Jie Na, Wenbin Ma, Dan Liu, Songyang Zhou
Summary: Induced crotonylation in hESCs resulted in differentiation into the endodermal lineage. This induced modification led to transcriptomic changes and decreased glycolysis in hESCs.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Chemistry, Multidisciplinary
Edward Kang, Yoon Young Choi, Su-Kyoung Chae, Jin-Hee Moon, Joon-Young Chang, Sang-Hoon Lee
ADVANCED MATERIALS
(2012)
Article
Chemistry, Multidisciplinary
Su-Kyoung Chae, Edward Kang, Ali Khademhosseini, Sang-Hoon Lee
ADVANCED MATERIALS
(2013)
Article
Chemistry, Multidisciplinary
Edward Kang, Jihee Ryoo, Gi Seok Jeong, Yoon Young Choi, Seung Min Jeong, Jongil Ju, Seok Chung, Shuichi Takayama, Sang-Hoon Lee
ADVANCED MATERIALS
(2013)
Article
Biochemical Research Methods
Bo Ram Lee, Kwang Ho Lee, Edward Kang, Dong-Sik Kim, Sang-Hoon Lee
Article
Engineering, Electrical & Electronic
Edward Kang, Dae Ho Lee, Chang-Beom Kim, Sung Ju Yoo, Sang-Hoon Lee
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2010)
Article
Biochemical Research Methods
Seung-A Lee, Da Yoon No, Edward Kang, Jongil Ju, Dong-Sik Kim, Sang-Hoon Lee
Review
Biochemical Research Methods
Yesl Jun, Edward Kang, Sukyoung Chae, Sang-Hoon Lee
Article
Chemistry, Multidisciplinary
Su-Kyoung Chae, Cho Hay Mun, Da-Yoon Noh, Edward Kong, Sang-Hoon Lee
Article
Chemistry, Physical
Edward Kang, Gi Seok Jeong, Yoon Young Choi, Kwang Ho Lee, Ali Khademhosseini, Sang-Hoon Lee
Article
Engineering, Biomedical
D. Y. Park, C. H. Mun, E. Kang, D. Y. No, J. Ju, S. H. Lee
Article
Ophthalmology
Young Ho Kim, Boram Lee, Edward Kang, Jaeryung Oh
Summary: The choroid thickness in the macular region was significantly thicker than in the extramacular area in normal subjects. Age had a significant impact on subfoveal choroidal thickness and nasal peripapillary choroidal thickness. The regional difference in choroidal thickness was influenced by sex, nasal peripapillary choroidal thickness, and macular neovascularization group.
CURRENT EYE RESEARCH
(2021)
Article
Multidisciplinary Sciences
Young Ho Kim, Boram Lee, Edward Kang, Jaeryung Oh
Summary: According to choroidal thickness profiles, eyes with AMD and PSD can be classified into different clusters, suggesting that pachydrusen may share a common pathogenesis with PSD.
SCIENTIFIC REPORTS
(2021)
Article
Ophthalmology
Young Ho Kim, Boram Lee, Edward Kang, Jaeryung Oh
Summary: Subfoveal choroidal thickness was greater in acute CSC group compared to other groups while peripapillary choroidal thickness was significantly higher in acute and chronic CSC groups than in controls. Choroidal vascularity index outside the macula was increased in all eyes with both current and past history of CSC, suggesting potential implications for choroidal characteristics.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2021)
Article
Ophthalmology
Edward Kang, Suk-Gyu Ha, Seung-Hyun Kim
Summary: This study investigated the correlation between near work and delayed-onset consecutive esotropia after surgery for intermittent exotropia. The frequency and hours of near work were found to be significantly associated with the occurrence of esotropia, suggesting the importance of closely monitoring near work activities postoperatively.
JOURNAL OF THE KOREAN OPHTHALMOLOGICAL SOCIETY
(2021)
