Journal
ELECTROPHORESIS
Volume 32, Issue 20, Pages 2904-2910Publisher
WILEY
DOI: 10.1002/elps.201100184
Keywords
Human serum; ILs-SDS-PAGE; Ionic liquids; Protein separation
Funding
- National Basic Research Program of China (973 Program) [2007CB914101]
- National Nature Science Foundation of China [20875009]
- Ministry of Education of China
- Nursery Fund for Outstanding Doctoral Dissertation
- Beijing Institute of Technology
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Ionic liquid (IL)-assisted sodium dodecyl sulfate polyacrylamide gel electrophoresis (ILs-SDS-PAGE) was presented to improve protein separation. ILs were employed during the preparation process of polyacrylamide gel, then the modified gel was used for commercial protein marker, binary bovine serum albumin/lysozyme (BSA/Lyz) and human serum separation. The influence of ionic liquid concentration, cation alkyl chain length, cation and anion types on proteins separation were investigated. The results showed that ILs played a role in improving some protein separation, and ILs-SDS-PAGE provided higher resolution and separation efficiency than ordinary SDS-PAGE for low and middle relative molecular mass proteins in human serum. In addition, the principle of ILs-SDS-PAGE was discussed and the comparison of ILs-SDS-PAGE with ordinary SDS-PAGE and Native PAGE was made.
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