Article
Biochemical Research Methods
Mengqi Luo, Tao Su, Qingyuan Cheng, Xue Zhang, Fei Cai, Zaiwen Yin, Fuping Li, Hao Yang, Fang Liu, Yong Zhang
Summary: In this study, the researchers used GlycoTCFM to profile the O-glycoproteome of human sperm and seminal plasma, revealing novel O-glycoproteins and their potential roles in sperm function and male infertility. The study also created a comprehensive O-glycoprotein database, providing valuable resources for further functional studies.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Jingfu Zhao, Ehwang Song, Yifan Huang, Aiying Yu, Yehia Mechref
Summary: This study delves into the microheterogeneity of glycosylation from four different clades of gp120s, detecting aberrant glycosylation patterns on gp120 coming from different clades, viral sequences, and host cells. The results may enhance understanding of how glycans participate in the antibody neutralizing process targeting glycosylation sites.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Chemistry, Analytical
Xianbin Meng, Lijie Li, Xiayan Wang
Summary: The development of an integrated strategy and software tools for constructing species-specific glycan mass lists has greatly facilitated the analysis of intact glycopeptides.
Article
Immunology
Yong Zhang, Shanshan Zheng, Wanjun Zhao, Yonghong Mao, Wei Cao, Wenjuan Zeng, Yueqiu Liu, Liqiang Hu, Meng Gong, Jingqiu Cheng, Younan Chen, Hao Yang
Summary: This study presents a hybrid dissociation technique for analyzing the glycosylation of highly glycosylated proteins, improving the performance of N/O-glycosylation analysis of the HIV-1 Env protein gp120. The method identified more glycopeptides and produced more comprehensive fragment ions for accurate glycosite assignment, demonstrating the potential for understanding HIV-1 infection and developing vaccines and drugs.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xueliang Lu, Junlong Wu, Yannan Qin, Jialin Liang, Hang Qian, Jidong Song, Chengjuan Qu, Ruiyu Liu
Summary: This study identified glycoproteins in ONFH hip cartilage and found differentially expressed glycoproteins related to cartilage degeneration processes. Analysis of KEGG pathways and protein-protein interactions indicated that these significantly differential glycoproteins are associated with multiple signaling pathways and protein biosynthesis processes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Environmental Sciences
Vincent Dufour, Laure Wiest, Sylvain Slaby, Francois Le Cor, Lucile Auger, Olivier Cardoso, Laurence Curtet, Laure Pasquini, Xavier Dauchy, Emmanuelle Vulliet, Damien Banas
Summary: Monitoring pesticides in small organisms in the environment requires sensitive analytical methods. This study developed a miniaturized extraction protocol for small biotic samples, allowing for the characterization of multiple pesticides and transformation products. The method showed high extraction rates and recoveries, making it an effective tool for analyzing polar pesticides in small organisms.
Article
Biochemistry & Molecular Biology
Clizia Chinello, Noortje de Haan, Giulia Capitoli, Barbara Trezzi, Antonella Radice, Lisa Pagani, Lucrezia Criscuolo, Stefano Signorini, Stefania Galimberti, Renato Alberto Sinico, Manfred Wuhrer, Fulvio Magni
Summary: Podocyte injury and proteinuria in idiopathic membranous nephropathy (IMN) are caused by an autoimmune reaction against podocyte antigens. This study systematically analyzed the IgG Fc glycosylation landscape in IMN patients and identified several glycan traits associated with the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Martina Pirro, Yassene Mohammed, Arnoud H. de Ru, George M. C. Janssen, Rayman T. N. Tjokrodirijo, Katarina Madunic, Manfred Wuhrer, Peter A. van Veelen, Paul J. Hensbergen
Summary: Developments in mass spectrometry (MS)-based analyses of glycoproteins have been crucial in studying glycosylation changes related to diseases. The characteristic pattern of oxonium ions in glycopeptide fragmentation spectra can help assign different sets of glycopeptides, particularly distinguishing between O-GalNAc and O-GlcNAc. By analyzing the relative intensities of oxonium ions, accurate assignment of glycopeptides can be achieved even in complex proteomics datasets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Christopher Burgoyne, Rob Smith
Summary: Glycans are important in biochemical processes, but the computational interpretation of glycans in mass spectrometry analysis can be challenging. The C++ implementation of the Sweet-SEQer algorithm, called C-SEQer, outperforms the original algorithm in terms of time and memory usage, making glycan analysis more efficient and effective. The implementation is freely available with an MIT license.
JOURNAL OF PROTEOME RESEARCH
(2021)
Review
Biochemical Research Methods
Jonas Carneiro Cruz, Israel Donizeti de Souza, Fernando Mauro Lancas, Maria Eugenia Costa Queiroz
Summary: Miniaturized liquid chromatography (LC) is recognized as an important analytical method in various research fields, offering higher resolution and lower consumption. However, limitations such as tubing clogging and small sample volumes hinder high throughput and sensitive analyses. Online coupling of sample preparation techniques can overcome these limitations and improve analytical efficiency.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Environmental Sciences
Alexandra K. Richardson, Rachel C. Irlam, Helena Rapp Wright, Graham A. Mills, Gary R. Fones, Stephen R. Stuerzenbaum, David A. Cowan, David J. Neep, Leon P. Barron
Summary: This study presents a miniaturized workflow for identification and monitoring of contaminants of emerging concern (CECs). It discusses the development of a low-cost small 3D-printed passive sampler device and a highly sensitive liquid chromatography-tandem mass spectrometry method. The field testing of the new 3D-PSD showed the successful identification of a higher number of CECs compared to traditional water samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Multidisciplinary
Luyao Liu, Lei Liu, Yan Wang, Zheng Fang, Yangyang Bian, Wenyao Zhang, Zhongyu Wang, Xianchun Gao, Changrui Zhao, Miaomiao Tian, Xiaoyan Liu, Hongqiang Qin, Zhimou Guo, Xinmiao Liang, Mingming Dong, Yongzhan Nie, Mingliang Ye
Summary: The lack of efficient biomarkers for early detection of gastric cancer is a major problem, and this study presents a highly robust N-glycoproteomics platform for large-scale analysis of glycosylation changes in gastric cancer. The platform identifies candidate biomarkers and demonstrates improved diagnostic performance compared to traditional markers. These findings highlight the value of analyzing aberrant glycans for cancer detection.
Article
Chemistry, Analytical
Frederic Jeanroy, Clothilde Comby-Zerbino, Claire Demesmay, Vincent Dugas
Summary: This study investigates the use of a new miniaturized affinity column to capture and elute high-affinity sequences from low molecular weight heparin for further characterization. Direct on-line analysis using MALDI-TOF MS was performed by applying the elution volume from the miniaturized affinity column directly to the MALDI plate.
ANALYTICA CHIMICA ACTA
(2023)
Article
Oncology
Daisuke Takakura, Shoko Ohashi, Noritoshi Kobayashi, Motohiko Tokuhisa, Yasushi Ichikawa, Nana Kawasaki
Summary: Aberrant glycosylation, a prominent feature of cancer, can be targeted to improve cancer biomarkers and assess metastasis risks. Researchers developed a targeted O-glycoproteomics method using serum specimens and identified 44 O-glycoforms associated with advanced colorectal cancer. These glycoforms could serve as promising markers for the detection of advanced CRC.
FRONTIERS IN ONCOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Maria J. Sebastia, Lara Marcos-Silva, Patricia Gomes-Alves, Paula M. Alves
Summary: Stem cell research has raised expectations for cardiac regenerative therapeutic approaches, but translating research and pre-clinical data into effective treatments remains challenging.
BIOTECHNOLOGY ADVANCES
(2021)
Editorial Material
Biochemical Research Methods
Iulia M. Lazar
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Biochemical Research Methods
Jingren Deng, Iulia M. Lazar
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2016)
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Biochemistry & Molecular Biology
Mihaela Babiceanu, Fujun Qin, Zhongqiu Xie, Yuemeng Jia, Kevin Lopez, Nick Janus, Loryn Facemire, Shailesh Kumar, Yuwei Pang, Yanjun Qi, Iulia M. Lazar, Hui Li
NUCLEIC ACIDS RESEARCH
(2016)
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Multidisciplinary Sciences
Iulia M. Lazar, Ina Hoeschele, Juliana de Morais, Milagros J. Tenga
SCIENTIFIC REPORTS
(2017)
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Chemistry, Analytical
Wooram Lee, Iulia M. Lazar
ANALYTICAL CHEMISTRY
(2014)
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Oncology
Milagros J. Tenga, Iulia M. Lazar
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Biochemical Research Methods
Iulia M. Lazar, Jingren Deng, Fumio Ikenishi, Alexandru C. Lazar
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Biochemical Research Methods
Xu Yang, Iulia M. Lazar
JOURNAL OF PROTEOME RESEARCH
(2014)
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Biochemical Research Methods
Jingren Deng, Morgan H. Julian, Iulia M. Lazar
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2018)
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Nanoscience & Nanotechnology
Lulia M. Lazar, Jingren Deng, Mark A. Stremler, Shreya Ahuja
MICROSYSTEMS & NANOENGINEERING
(2019)
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Multidisciplinary Sciences
Iulia M. Lazar, Arba Karcini, Shreya Ahuja, Carly Estrada-Palma
SCIENTIFIC REPORTS
(2019)
Review
Chemistry, Analytical
Iulia M. Lazar, Nicholas S. Gulakowski, Alexandru C. Lazar
ANALYTICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Iulia M. Lazar, Arba Karcini, Joshua R. S. Haueis
Summary: The study aimed to explore the involvement of cell-membrane proteins in cancer development and identify potential therapeutic targets using a membrane proteome/cancer hallmark perspective. The findings demonstrated the significant role of cell-membrane proteins in cancer development and highlighted the potential for novel therapeutic interventions.
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Multidisciplinary Sciences
Hetal A. Sarvaiya, Iulia M. Lazar
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Biotechnology & Applied Microbiology
Iulia M. Lazar
CHIMICA OGGI-CHEMISTRY TODAY
(2014)