Journal
ELECTROPHORESIS
Volume 31, Issue 2, Pages 388-395Publisher
WILEY
DOI: 10.1002/elps.200900315
Keywords
CE; Human growth hormone; MS; TOF
Funding
- Swiss Federal Council of Sports (Magglingen, Switzerland)
- Dr. Matthias Kamber, Director of Antidoping Switzerland (Bern, Switzerland)
- The World Anti-Doping Agency (Montreal, Canada)
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CE is a powerful analytical tool used to separate intact biomolecules such as proteins. The coupling of CE with TOF/MS produces a very promising method that can be used to detect and identify proteins in different matrices. This paper describes an efficient, rapid, and simple CE-ESI-TOF/MS procedure for the analysis of endogenous human growth hormone and recombinant human growth hormone without sample preparation. Operational factors were optimized using an experimental design, and the method was successfully applied to distinguish human growth hormone and recombinant human growth hormone in unknown samples.
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