Article
Chemistry, Analytical
Lvxia Zhang, Hui Ouyang, Dan Zhang, Zhifeng Fu
Summary: Novel cobalt-based metal-organic frameworks (Co MOFs) were synthesized and showed superior catalytic performance in the chemiluminescent (CL) reaction. The Co MOFs accelerated the decomposition of H2O2 and production of oxygen radicals, leading to a sensitive method for immunoassay of aflatoxin B-1. The results demonstrated the reliability of Co MOFs-based CL immunoassay for detecting herbal medicine samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Yang Jiang, Rong Li, Wenyu He, Qian Li, Xia Yang, Sijia Li, Wanqiao Bai, Yan Li
Summary: A novel electrochemiluminescence biosensor was developed for the determination of miRNA-21, utilizing a hybrid luminescent composite as the luminophore and a signal amplification strategy. The biosensor exhibited strong and stable ECL signal, and had a wide linear range and low detection limit for miRNA-21. This biosensor shows promise for clinical diagnosis and disease prognosis analysis.
Article
Nanoscience & Nanotechnology
Rui Yang, Chuanbao Yuan, Wenxuan Dong, Yueran Ren, Yangyang Xue, Hua Cui
Summary: In this study, magnetic nanoparticles with excellent catalytic performance and fast magnetic separation ability were synthesized and used for constructing a label-free chemiluminescence immunosensor for antioxidant detection. The developed immunosensor exhibited a wide linear range and low detection limit, making it suitable for clinical diagnosis.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Xin-Tao Wang, Yi-Rong Jiang, Li-Yan Huang, Yi-Xin Gu, Xiao-Qin Huang, Ai-Jun Wang, Pei-Xin Yuan, Jiu-Ju Feng
Summary: Metal-organic frameworks were used to enhance the ECL responses of N-(aminobutyl)-N-(ethylisoluminol) and develop a high-performance ECL biosensor for the detection of chloramphenicol.
Article
Chemistry, Multidisciplinary
David Nugroho, Chayanee Keawprom, Saksit Chanthai, Won-Chun Oh, Rachadaporn Benchawattananon
Summary: This study synthesized luminol carbon dots (N-CDs) from coconut water and ethanol, which are capable of detecting latent fingerprints on non-porous surfaces and can be easily detected under a UV lamp. The research discovered that N-CDs have a small size and a higher fluorescence intensity compared to standard ones.
Article
Chemistry, Analytical
Yuexin Wang, Rui Chen, Bo Shen, Cai Li, Junman Chen, Yanshuang Wang, Shen Tian, Xuemiao Li, Nini Luo, Rui Liu, Shijia Ding, Chuanlong Zhu, Qianfeng Xia
Summary: A multifunctional catalytic nanomaterial (Co-MOF@AuNP@ABEI) has been developed to improve the detection sensitivity of an electrochemiluminescent (ECL) biosensor. The combination of Co-MOF@AuNP@ABEI with the ECL system and a magnetic walking nanomachine amplification strategy enables ultrasensitive ECL assays for Burkholderia pseudomallei.
Article
Chemistry, Analytical
Xin Chen, Jingxiu Mao, Fuyu Wen, Xueqin Xu
Summary: A rapid and simple method utilizing capillary electrophoresis and chemiluminescence detection has been developed for the determination of phenolic acids in botanical drugs. Under optimized conditions, the analytes can be well separated within 7 minutes, with detection limits ranging from 35-58 mu g/mL and quantification limits ranging from 117-193 mu g/mL. This method has been successfully applied for the quantitative determination of phenolic acids in Lonicera japonica flos and Rhizoma Chuanxiong.
ANALYTICAL LETTERS
(2021)
Article
Chemistry, Analytical
Mingquan Guo, Jiangnan Shu, Dexin Du, Mohammad A. Haghighatbin, Di Yang, Zhiping Bian, Hua Cui
Summary: A novel ECL nanoluminophore was synthesized in this work for detecting cardiac troponin I with a wide linear dynamic range and low detection limit. The sensor can accurately determine the target analyte based on the ratio of three ECL peaks and offers self-correction analysis capabilities.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Miklos Szabo, Csenge Filep, Mate Nagy, Daniel Sarkozy, Marton Szigeti, Edit Sperling, Eszter Csanky, Andras Guttman
Summary: Omalizumab, a glycoprotein based biotherapeutics, is widely used to improve asthma symptoms and reduce corticosteroid usage. The glycosylation of such drugs plays a crucial role in their effectiveness and clearance time, with comprehensive analysis needed during production and release.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Pingjing Li, Lulu Wang, Rui Guo, Huatao Feng, Ya Ji, Si Ying Lim, Bao Hui Ng, Anna Karen Carrasco Laserna, Shaheer Khan, Shiaw-Min Chen, Sam Fong Yau Li
Summary: Recombinant protein biopharmaceuticals are important in current drug development, and the glycosylation profile of these proteins plays a crucial role in their quality. This study developed a CE-LIF method for accurate glycan identification by cross-identification using two capillary coatings and three labeling dyes. The use of homemade PVA and commercial coatings showed similar separation profiles, and the combination of fluorescent dyes provided better accuracy.
Article
Chemistry, Analytical
Manli Wang, Jiangnan Shu, Shanshan Wang, Aihua Lyu, Yisha Wang, Dabing Huang, Hua Cui
Summary: A novel magnetic nanomaterial, Fe3O4@Au/Ag/ABEI/Co2+, with strong and stable chemiluminescence (CL) emission was synthesized. A label-free CL immunosensor for exosome detection was established based on this nanomaterial, exhibiting excellent analytical performance. The immunosensor has the potential for early cancer diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Robert Farsang, Gabor Jarvas, Andras Guttman
Summary: The possible sample losses during the purification process of fluorophore labeling reaction mixture in capillary gel electrophoresis were investigated. A normal polarity capillary zone electrophoresis separation mode and Trishexanoic acid running buffer at basic pH were applied for purification. The method effectively separated the labeled sample components of interest and showed electrophoretic profile differences caused by the cleanup process.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Steven J. Blom, Timothy U. Connell, Egan H. Doeven, David J. Hayne, Emily Kerr, Luke C. Henderson, Paul S. Francis
Summary: Examining heteroleptic iridium(III) complexes as luminophores for cathodic electrogenerated chemiluminescence (ECL) with peroxydisulfate co-reactant revealed that these water-soluble complexes have more positive reduction potentials compared to the archetypal tris(2,20-bipyridine)ruthenium(II) luminophore. The observed shifts in potential favor cathodic ECL by reducing oxidative quenching and improving the stability of the reduced metal complex in aqueous solvent. The ECL intensities increased with repeated scanning or pulsing of the potential, suggesting deposition of the less-soluble reduced species at the electrode surface. The iridium(III) complex 'Ir4', containing cyclometalating ligands derived from the 1-methyl-4-(2MODIFIER LETTER PRIME-pyridyl)pyridinium cation, exhibited intense ECL in aqueous solution with peroxydisulfate co-reactant, comparable to [Ru(bpz)3]2, but with green luminescence instead of the conventional red emissions.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Bin Yang, Thanh Duc Mai, Nguyet Thuy Tran, Myriam Taverna
Summary: This study presents a new integrated approach for fluorescent labeling and analysis of N-glycans using capillary electrophoresis. The approach utilizes the capillary as both a microreactor for labeling and a separation channel for analysis, allowing for automation and improved efficiency. The method was successfully applied to analyze fluorescently labeled N-linked oligosaccharides from human immunoglobulin G and rituximab.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Oncology
Hirotake Kodama, Tohru Yoneyama, Toshikazu Tanaka, Daisuke Noro, Yuki Tobisawa, Hayato Yamamoto, Shinichiro Suto, Shingo Hatakeyama, Kazuyuki Mori, Takahiro Yoneyama, Yasuhiro Hashimoto, Ikuko Kakizaki, Shigeyuki Nakaji, Chikara Ohyama
Summary: The study evaluated the diagnostic performance of N-glycosylation signatures of immunoglobulins for detecting urothelial carcinoma, including bladder cancer and upper urinary tract UC. The results showed that N-glycosylation signatures of Igs provided significantly better clinical performance in discriminating BCa and UTUC compared to other clinical factors, and could be a promising approach for the diagnosis of UC.
