Review
Chemistry, Analytical
Petr Tuma
Summary: This review article covers the historical development and current techniques of using capillary and chip electrophoresis with contactless conductivity detection for amino acid determination. It discusses theory of conductivity detection, optimization of background electrolyte, chiral separation, multidimensional separation, and the use of multidetection systems. Additionally, it highlights clinical applications and modern sample treatment techniques.
Review
Biochemical Research Methods
Abdalla A. Elbashir, Abdelbagi Osman, Mohammed Elawad, Abobakr K. Ziyada, Hassan Y. Aboul-Enein
Summary: The coupling of capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) is a convenient analytical method for the determination of small molecules that lack chromogenic or fluorogenic groups. This article demonstrates the use of CE-C4D in the determination of inorganic and organic ions, as well as amino acids, in the biomedical field. Attention is given to the composition of the background electrolyte, sample treatment procedures, and the utilization of multi-detection systems. Tables summarizing highly developed CE-C4D methods and the achieved figures of merit are included. Lastly, concluding remarks and perspectives are provided.
Article
Biochemical Research Methods
Petr Tuma, Tomas Hlozek, Jana Kamisova, Jan Gojda
Summary: In this study, capillary electrophoresis with contactless conductivity detection was used to determine branched chain amino acids, alanine and glutamine in human plasma. Patients with pancreatic cancer and cancer cachexia syndrome were found to have lower levels of circulating BCAAs and glutamine, as well as the loss of their insulin-dependent suppression.
Article
Biochemical Research Methods
Ying Wang, ZhiRong Li, RongXiang Chen
Summary: A comprehensive quantification method for amino acids and metabolites related to arginine metabolism in rat plasma was developed using hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS). The method showed good linear regression, low limits of detection, and high precision and accuracy. It was successfully applied to study dynamic alterations in the plasma of rats with neuropathic pain, providing insights into the mechanism between metabolite changes and clinical disease.
JOURNAL OF CHROMATOGRAPHIC SCIENCE
(2023)
Article
Biochemical Research Methods
Kemilly M. P. Pinheiro, Lucas M. Duarte, Marcella F. Rodrigues, Boniek G. Vaz, Iris Medeiros Junior, Rogerio M. Carvalho, Wendell K. T. Coltro
Summary: This study introduces the use of a microchip electrophoresis device with capacitively coupled contactless conductivity detection for analyzing naphthenic acids in produced water. The developed methodology achieved efficient separation and detection of the target acids in a short time with high resolution. The method demonstrated good linear correlation, satisfactory repeatability, and inter-day precision.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Saara Mikkonen, Asa Emmer
Summary: CE-(CD)-D-4 methods were used for analyzing amino acids, successfully identifying 20 proteinogenic AAs and analyzing free AAs in samples of mammalian cell culture supernatant for the first time. By combining results from two CE methods after dilution, changes in the concentrations of 17 AAs during CHO cell cultivation could be monitored efficiently.
Article
Chemistry, Analytical
Zheng Ren Wang, Ming-Mu Hsieh, Yi-Yang Sung
Summary: The study presents a highly efficient method for detecting underivatized adamantane analogs by integrating UADLLME, FASS-CE, and C4D technologies, achieving satisfactory results through online concentration during CE separation and proving the feasibility and accuracy for determination of MT, AT, and RT in human serum and urine.
MICROCHEMICAL JOURNAL
(2021)
Article
Immunology
Jing Xie, Ying Wang, Qi Zhong, Shun-jie Bai, Chan-juan Zhou, Tian Tian, Jian-jun Chen
Summary: This study investigated the relationships between microbial metabolites in feces and neurotransmitters in the prefrontal cortex of depressed mice using targeted metabolomics. The results identified differential microbial metabolites and neurotransmitters and found significant correlations between them. The study suggests that disturbances in microbial metabolites, especially amino acids, may affect neurotransmitter levels and contribute to the onset of depression.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Wei Ji, Hui Yuan, Bin Xue, Sarah Guerin, Hui Li, Lei Zhang, Yanqing Liu, Linda J. W. Shimon, Mingsu Si, Yi Cao, Wei Wang, Damien Thompson, Kaiyong Cai, Rusen Yang, Ehud Gazit
Summary: This study reports a co-assembly approach to achieve the solid-state stacking transformation of supramolecular organization, resulting in enhanced physical properties. Diverse molecular packings can be obtained by different derivatives.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Benneth Ben-Azu, Christian Uruaka, Abayomi M. Ajayi, Thiophilus Aghogho Jarikre, Kingsley E. Nwangwa, Kingsley C. Chilaka, Bienose S. Chijioke, Marymagdalene G. Omonyeme, Chineye B. Ozege, Emmanuella C. Ofili, Ebidenara B. Warekoromor, Nwanneka L. Edigbue, Ufoma Esiekpe, Dabrechi E. Akaenyi, Gladys O. Agu
Summary: Schizophrenia is a severe neuropsychiatric disorder caused by complex neurobiological changes. Taurine has shown potential in reducing symptoms and protecting against schizophrenia through modulation of neurochemicals and neurotrophic factors, and inhibition of inflammatory cytokines.
NEUROCHEMICAL RESEARCH
(2023)
Article
Plant Sciences
Chu Wu, Yun Wang, Honggang Sun
Summary: In this study, targeted and untargeted metabolomics analysis were conducted to evaluate the drought tolerance of Pinus taeda seedlings. The results showed that certain sugars, phytohormones, and amino acids increased significantly in the roots and needles of water-stressed seedlings. Additionally, specific metabolites and biosynthesis pathways were found, providing insights for breeding drought-tolerant P. taeda cultivars.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemical Research Methods
Jiajia Shen, He Wang, Haoran Huang, Haofeng Li, Changjian Li, Caixia Yan, Tengjie Yu, Huimin Guo, Kangrui Hu, Yisha Du, Hong Sun, Lin Xie, Pingfei Fang, Yan Liang
Summary: The study proposed an all-in-one liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for efficiently analyzing neurotransmitters and amino acids. Through research on absorption solvents and Collision Energy Defect strategy, the study achieved quantitative results with high sensitivity, accuracy, and noticeable recovery effects.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemistry & Molecular Biology
Shanalee C. James, Karl Fraser, Janine Cooney, Catrin S. Guenther, Wayne Young, Richard B. Gearry, Phoebe E. Heenan, Tania Trower, Jacqueline I. Keenan, Nicholas J. Talley, Warren C. McNabb, Nicole C. Roy
Summary: This study aimed to compare the circulating concentrations of amino acids and neurotransmitters between healthy participants and those with functional gastrointestinal disorders (FGIDs). The results showed significant differences in branched-chain amino acids, ornithine, and alpha-aminobutyric acid between healthy controls and FGID groups. No other significant differences were observed for the neurotransmitters or any other amino acids analyzed.
Article
Chemistry, Analytical
Kirsten J. Dehning, Moritz Hitzemann, Alexander Gossmann, Stefan Zimmermann
Summary: Amino acids play a vital role in life as they are essential components of proteins and necessary for cell growth and maintenance. This study introduces a split-ring resonator as a sensitive and simple detector for amino acids. The split-ring resonator uses the electromagnetic properties of a liquid sample to determine the resonance frequency, and initial measurements show detection limits for tested amino acids ranging from 105 μM to 1564 μM. With an envelope detector, the split-ring resonator can be used in ion chromatography and achieve detection limits of 485 μM for aspartic acid and 956 μM for lysine at a flow rate of 0.5 mL/min.
Review
Chemistry, Applied
Petr Tuma, Frantisek Opekar, Pavel Dlouhy
Summary: This paper provides a comprehensive survey of using capillary and microchip electrophoresis with contactless conductivity detection for the analysis of drinking water, beverages, and food items. It highlights the analysis of food on microchips and modern trends such as dual-opposite injection. CE/(CD)-D-4 is characterized by its universality, analysis speed, simple sample preparation, and minimal sample consumption.
Review
Chemistry, Analytical
Petr Tuma
Summary: This review article covers the historical development and current techniques of using capillary and chip electrophoresis with contactless conductivity detection for amino acid determination. It discusses theory of conductivity detection, optimization of background electrolyte, chiral separation, multidimensional separation, and the use of multidetection systems. Additionally, it highlights clinical applications and modern sample treatment techniques.
Article
Biochemical Research Methods
Petr Tuma, Martin Jacek, Blanka Sommerova, Pavel Dlouhy, Radka Jarosikova, Jitka Husakova, Veronika Woskova, Vladimira Fejfarova
Summary: The determination of broad-spectrum antibiotics AMX and CTZ in blood serum and microdialysates using CE with CD-D-4 detection is described. The method provides baseline separation, low detection limits, and short migration times. It is suitable for monitoring the treatment of diabetic foot patients and pharmacokinetic studies.
Review
Chemistry, Applied
Petr Tuma, Frantisek Opekar, Pavel Dlouhy
Summary: This paper provides a comprehensive survey of using capillary and microchip electrophoresis with contactless conductivity detection for the analysis of drinking water, beverages, and food items. It highlights the analysis of food on microchips and modern trends such as dual-opposite injection. CE/(CD)-D-4 is characterized by its universality, analysis speed, simple sample preparation, and minimal sample consumption.
Review
Chemistry, Analytical
Petr Tuma
Summary: Contactless conductivity detection (C4D) is a universal detection technique that plays a significant role in the analysis of clinical samples. It is particularly sensitive to low molecular weight biogenic substances and can directly quantify them in their native form, making it widely applicable.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Veronika Solinova, Petr Tuma, Maria Butnariu, Vaclav Kasicka, Dusan Koval
Summary: We present a method for finely adjustable electroosmotic flow (EOF) velocity in cathodic direction for the optimization of separations in capillary electrophoresis. The method involves surface modification of the separation fused silica capillary using a covalently attached copolymer to control the charge density and achieve adjustable EOF. The properties of EOF in the treated capillaries are observed and the importance of adjustable EOF in separations is demonstrated.
Article
Clinical Neurology
Annunziata Guarino, Barbara Bettegazzi, Nimra Aziz, Mario Barbieri, Daniela Bochicchio, Lucia Crippa, Pietro Marino, Maddalena Sguizzato, Marie Soukupova, Silvia Zucchini, Michele Simonato
Summary: Temporal lobe epilepsy often appears after an initial epileptogenic insult and may be preventable. This study tested the antioxidant agent 7,8-dihydroxyflavone (7,8-DHF) as an anti-epileptogenic drug and found that low-dose 7,8-DHF effectively reduced the frequency of seizures in the experimental model.
Article
Chemistry, Analytical
Petr Tuma, Blanka Sommerova, Dusan Koval, Michaela Siklova, Michal Koc
Summary: This study presents a sensitive electrophoretic method for determining 3-hydroxybutyrate (3HB) levels, which is an indicator of human ketogenesis. By using a specially coated capillary and optimizing the separation conditions, baseline separation of 3HB from other components in human serum can be achieved. The method shows low limits of detection and quantification, making it suitable for monitoring physiological 3HB levels.
Article
Psychiatry
Mario Barbieri, Micaela Tirri, Sabrine Bilel, Raffaella Arfe, Giorgia Corli, Beatrice Marchetti, Lorenzo Caruso, Marie Soukupova, Virginia Cristofori, Giovanni Serpelloni, Matteo Marti
Summary: This study investigated the in vivo pharmacological activity and brain electrical effects of JWH-073, a synthetic cannabinoid. The results showed that JWH-073 temporarily decreased motor activity and visual sensorimotor responses in mice, while also affecting memory function and brain electrical responsiveness. These findings suggest that JWH-073 poses a subtle threat to consumers who underestimate its potential harm.
FRONTIERS IN PSYCHIATRY
(2022)
Article
Engineering, Chemical
Tomas Hlozek, Tereza Bosakova, Zuzana Bosakova, Petr Tuma
Summary: Effective elimination of estrogens from water sources and wastewater is crucial. Hydrophobic eutectic solvents (ES) show high extraction efficiency, with menthol and octanoic acid in a 1:1 ratio being the most effective for all estrogens.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Review
Chemistry, Analytical
Petr Tuma
Summary: This review evaluates the progress made in the past decade in on-line, at-line, and in-line sample treatment coupled with capillary and microchip electrophoresis. The first part discusses various types of flow-gating interfaces (FGI) and their fabrication using materials like polydimethylsiloxane and commercially available fittings. The second part focuses on the coupling of electrophoresis with different extraction techniques, highlighting modern approaches such as supported liquid membrane extraction and microdialysis with high spatial and temporal resolution. The final section explores the design of sequential electrophoretic analyzers and the fabrication of SPE microcartridges with specialized sorbents. Applications of these techniques range from monitoring metabolites and proteins in living organisms to analyzing nutrients and waste compounds in food and wastewater.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Petr Tuma, Blanka Sommerova, Dusan Koval, Michaela Siklova, Michal Koc
Summary: In this study, capillary electrophoresis was used to determine minor nitrogen metabolites, including creatine, 2-aminobutyric acid (2-AB), and acetyl-carnitine (Ac-carn), in human plasma. Baseline separation of these metabolites was achieved using a 6% PAMAPTAC coated fused silica capillary and a counter-current mode. Plasma levels of these metabolites were monitored during a 60-hour fasting period, and significant differences were observed compared to baseline levels.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Frantisek Opekar, Petr Tuma
Summary: A miniature probe for electromembrane extraction was developed. It was made of polypropylene hollow fiber with a supported liquid membrane. The probe parameters were tested for extraction of creatinine and basic amino acids with promising results in increased sensitivity. The probe is simple to prepare and can be used for all-day measurements.
Review
Endocrinology & Metabolism
Vladimira Fejfarova, Radka Jarosikova, Jan Polak, Blanka Sommerova, Jitka Husakova, Veronika Woskova, Michal Dubsky, Petr Tuma
Summary: Diabetic foot is a serious complication caused by infection and ischaemia, requiring prompt and aggressive treatment to avoid amputation. Peripheral arterial disease therapy can be easily verified using ultrasound or other examinations, but infection treatment success is difficult to establish. Intravenous antibiotics are recommended for moderate or serious infections, and microdialysis techniques show promise in determining antibiotic levels in diabetic foot lesions.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Chemistry, Analytical
Frantisek Opekar, Petr Tuma
Summary: This article introduces a method for extracting the anesthetic ketamine using electromembrane extraction coupled with capillary electrophoresis. The extraction occurs in a flow-through extraction mini probe, consisting of a porous hollow fiber filled with nitrophenyloctyl ether as extraction liquid. The pH of the sample solution does not affect the extraction efficiency, and the calibration dependence is linear in both model samples and urine. The LOD values are around 1 µM.
MICROCHEMICAL JOURNAL
(2023)
Article
Anesthesiology
J. Malek, P. Tuma
Summary: The article provides an overview of the transition of curare from a toxic hunting arrow substance to a medicinal muscle relaxant. It also reviews other related substances and acknowledges individuals who played a significant role in its history. Additionally, the article discusses the development of muscle relaxant antidotes, the discovery of the nicotinic acetylcholine receptor, and the misuse of muscle relaxants outside of operating rooms.
ANESTEZIOLOGIE A INTENZIVNI MEDICINA
(2022)
