Journal
ELECTROPHORESIS
Volume 30, Issue 24, Pages 4292-4299Publisher
WILEY
DOI: 10.1002/elps.200900382
Keywords
Chip electrophoresis; 1,2-Dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl); iminodiacetic acid)succinyl] (nickel salt); Human Rhinovirus; Liposome; Receptor
Funding
- Austrian Science Foundation [P19365]
- Austrian Science Fund (FWF) [P19365] Funding Source: Austrian Science Fund (FWF)
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In first attempts at elucidating the transfer of the RNA genome of a human Rhinovirus through lipid membranes in vitro we made use of liposomes decorated with recombinant receptors. This model system was characterized previously by CE but suffered from the requirement for inclusion of polyethylene glycol-modified lipids for reliable separations [Weiss, V. U., Bilek, G., Pickl-Herk, A., Blaas, D., Kenndler, E., Electrophoresis 2009, 30, 2123-2128.]. We here report the analysis of liposomes with a lipid composition much more similar to that of biological lipid bilayers. We found that vesicles containing and lacking this non-physiologic lipid differ significantly in their electrophoretic mobility (by factor 2) although the concentration of charge-bearing lipids in their bilayers is the same. We demonstrate that binding of a human Rhinovirus to the latter liposomes decorated with a cognate receptor can be analysed via electrophoresis on microchips; we support our results with transmission electron microscopy.
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