Journal
ELECTROANALYSIS
Volume 27, Issue 1, Pages 32-37Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201400365
Keywords
Peanuts; Aptamers; Impedance; Biosensors; Label-free
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Funding
- Research Council University of Antwerp (IWS BOF UA)
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The peanut allergen Ara h 1 belonging to the vicilin family of seed storage proteins is known to be a suitable marker for peanut detection in foods. In the present work, we suggest an electrochemical detection of Ara h 1 using a recently selected 80-base DNA aptamer. The detection strategy relies on insulation effect of a bulky protein captured by an immobilized aptamer. The electrodes were modified by the aptamer and characterized using electrochemical impedance spectroscopy (EIS). The aptamer surface density and analytical parameters of the calibration curves for Ara h 1 were compared with the system prepared with thrombin binding aptamer (TBA) and operated in the same conditions.
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