Journal
ELECTROANALYSIS
Volume 27, Issue 2, Pages 368-377Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201400387
Keywords
Putrescine; Biosensors; Putrescine oxidase; Screen-printed electrodes; Isothermal titration calorimetry
Categories
Funding
- Junta de Castilla y Leon [BU212A12-2]
- Ministerio de Ciencia e Innovacion (MICINN) [TEC2009-12029]
- MICINN
- Fondo Europeo de Desarrollo Regional (FEDER) (IN-NPACTO SERIBIO)
- MICINN scholarship FPI
- Portuguese Foundation for Science and Technology (FCT) [UI 195-2011-2013, Pest-OE/CTM/UI0195/2011]
- Fundação para a Ciência e a Tecnologia [PEst-OE/CTM/UI0195/2011] Funding Source: FCT
Ask authors/readers for more resources
Putrescine oxidase (PUO) from Micrococcus rubens has been characterized, as a biocatalytic component for the development of electrochemical biosensors for the determination of putrescine. PUO was immobilized by cross-linking on screen-printed carbon electrodes (SPCEs) containing tetrathiafulvalene (TTF) as mediator (TTF/SPCEs). Isothermal titration calorimetry was used for investigation of kinetic properties of immobilized PUO. These PUO/TTF/SPCEs showed a linear response range from 10 a 74 mu M, a capability of detection of 10.1 +/- 0.6 mu M (n=4, alpha=beta=0.05) and a precision of 6.7%. Octopus and zucchini samples have been analysed using HPLC as a reference technique.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
