4.5 Article

Multifunctional Mediating System Composed of a Conducting Polymer Matrix, Redox Mediator and Functionalized Carbon Nanotubes: Integration with an Enzyme for Effective Bioelectrocatalytic Oxidation of Glucose

Journal

ELECTROANALYSIS
Volume 25, Issue 12, Pages 2651-2658

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201300423

Keywords

Biofuel cells; Carbon nanotubes; Conducting polymers; Glucose oxidation; Modified electrodes

Funding

  1. Polish Ministry of Sciences and Higher Education
  2. National Center for Research and Development (NCBiR) [NR05-0017-10/2010 (PBR-11)]
  3. Foundation for Polish Science (FNP) through the Chair (Mistrz) and Program
  4. US National Institutes of Health [R15GM087662-01]
  5. University of Warsaw under the BST project

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A multifunctional mediating system for bioelectrocatalytic oxidation of glucose is described. It comprises a conducting polymer, poly(3,4-ethylenodioxythiophene), carbon nanotubes modified with 4-(pyrrole-1-yl) benzoic acid that provide carboxyl groups that aid immobilization of glucose oxidase in a conductive three-dimensional network, tetrathiafulvalene that mediates electron exchange with the enzyme. This composite produces a system that is capable of effective oxidation of glucose to gluconic acid at pH7. The current is concentration dependent to at least 60mM and maintains 77% of initial response for 35 days. Data supporting the utility of this system for electrochemical sensing and biofuel cell technology are presented.

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