Article
Chemistry, Multidisciplinary
Marjon Zamani, James M. Robson, Andy Fan, Michael S. Bono, Ariel L. Furst, Catherine M. Klapperich
Summary: Sexually transmitted infections, such as HIV and HPV, have a disproportionate impact on low-resource settings. Early diagnosis is crucial for managing HIV and preventing HPV-induced cervical cancer. A novel electrochemical platform combined with loop-mediated isothermal amplification and CRISPR-based detection shows promise for rapidly and cost-effectively detecting viral infections.
ACS CENTRAL SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Fangfei Yin, Haipei Zhao, Shasha Lu, Juwen Shen, Min Li, Xiuhai Mao, Fan Li, Jiye Shi, Jiang Li, Baijun Dong, Wei Xue, Xiaolei Zuo, Xiurong Yang, Chunhai Fan
Summary: The article introduces a DNA-encoded molecular classifier that can classify multidimensional molecular clinical data and has been tested in prostate cancer patients.
NATURE NANOTECHNOLOGY
(2023)
Article
Chemistry, Organic
Toby Lewis-Atwell, Piers A. Townsend, Matthew N. Grayson
Summary: In this study, the relative performances of different force fields for conformational searching of hydrogen-bond-donating catalyst-like molecules were compared. MM3, MMFFs, and OPLS3e consistently showed strong performances and are recommended for conformationally searching molecules structurally similar to those in this study.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Review
Chemistry, Physical
Mohamed Sharafeldin, James F. Rusling
Summary: Rapid and accurate diagnoses are crucial for efficient healthcare, enabling early disease detection, avoiding unnecessary treatment, and improving outcomes. Electrochemical techniques offer user-friendly, sensitive, and low-cost assays to support clinical applications by analyzing relevant disease biomarkers. Multiplexed electrochemical assays provide more accurate and precise diagnostics compared to single biomarker assays, leveraging the inherent sensitivity and portability of electrochemistry.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Review
Chemistry, Physical
Yuchan Yuan, Netzahualcoyotl Arroyo-Curras
Summary: Nucleic acid-based electrochemical (NBE) sensors offer real-time and reagent-free sensing capabilities by using affinity-based molecular detection. These sensors are modular and versatile, allowing the monitoring of various molecular targets by swapping recognition probes. However, their performance in vivo has not been rigorously validated relative to benchmark methods. This article highlights recent reports evaluating the in vivo performance of NBE sensors, aiming to inspire future translational efforts for clinical applications and commercial development.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Yao-Hua Liu, Ying-Ming Zhang, Hua-Jiang Yu, Yu Liu
Summary: The use of cucurbiturils (CBs) in biomacromolecular assemblies has made significant progress in various applications, including biosensors, biochemical reaction regulation, and disease treatment. CBs have also shown the ability to promote subcellular bioimaging in different organelles, demonstrating diverse applications in the field of supramolecular chemistry.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Obianuju A. Nwokolo, Brant Kidd, Te'Kara Allen, Alexander S. Minasyan, Suchitra Vardelly, Kristopher D. Johnson, Irina V. Nesterova
Summary: In this study, a memory-based sensor was designed based on the unique kinetic properties of nucleic acid quadruplexes to measure heat changes in small open systems. The sensors are biocompatible, operate homogeneously, and measure small heats released over long time periods, and have been successfully applied to water/propanol mixing and ligand/protein binding.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Chemistry, Physical
Alexander Shaver, Netzahualcoyotl Arroyo-Curras
Summary: Nucleic acid-based electrochemical sensors are versatile technology for detecting molecular targets. However, their long-term stability is a limiting factor. This article discusses methods to enhance stability and urges the field to develop innovative strategies.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Zijie Zhang, Payel Sen, Bal Ram Adhikari, Yingfu Li, Leyla Soleymani
Summary: Nucleic acids, particularly DNA, possess remarkable properties that make them ideal for use in electrochemical biosensors. This review discusses the historical evolution of nucleic acids as probes in electrochemical biosensors and provides specific examples of nucleic acid-based biosensors that have been evaluated for clinical use in infectious disease, cancer, or cardiovascular health.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Jinqi Deng, Chao Liu, Jiashu Sun
Summary: Extracellular vesicles (EVs) are nanovesicles derived from cells, carrying various molecular cargos and playing important roles in intercellular communication and physiological and pathological processes. DNA-based nanomaterials, with their excellent programmability and modifiability, have emerged as promising tools for detecting molecular cargos carried by EVs. This perspective summarizes recent advancements in EV analysis using different types of DNA-based nanomaterials, including linear DNA probes, DNA nanostructures, and hybrid DNA nanomaterials. The design, construction, advantages, and disadvantages of these DNA nanomaterials, as well as their performance for detecting EVs, are reviewed. The challenges and opportunities in the field of EV analysis by DNA nanomaterials are also discussed.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Physical
Jing Su, Yuqing Ke, Nokuzola Maboyi, Xiao Zhi, Sijia Yan, Fuwu Li, Bo Zhao, Xiaolong Jia, Shiping Song, Xianting Ding
Summary: The authors developed a CRISPR/Cas12a-powered DNA framework-supported electrochemical biosensing platform for ultrasensitive nucleic acid analysis without pre-amplification, showing incredible capability for sequence-specific targeting and detection.
Article
Chemistry, Analytical
Rui Zhang, Pu Wang, Yangyang Chang, Meng Liu
Summary: Rapid and sensitive detection of ATP in bioaerosols is crucial. The strategy of interfacing split DNA aptamers with MXene-based composite materials provides a versatile approach for the ubiquitous detection of biochemical targets in bioaerosols.
ANALYTICAL CHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Anas Ahmad, Mohammad Imran, Haseeb Ahsan
Summary: A biomarker is a measurable biological molecule that can serve as an index of normal or abnormal pathophysiology or the response to treatment. Different tissues in the body have distinct biomolecular make-ups called biomarkers, which include genes, proteins, and other biomolecules. Biomarkers can be objectively quantified through biochemical samples to evaluate an organism's exposure to normal or pathological processes or how it responds to drug interventions. Understanding the significance of biomarkers is crucial for efficient disease diagnosis and guiding treatment choices. Advances in omics technologies have allowed for the discovery of new biomarkers using various strategies like genomics, epigenetics, metabolomics, transcriptomics, lipid analysis, and protein studies. These biomarkers have been applied for screening normal and diseased tissue or serum samples and are valuable tools in pharmacology and therapeutics. This review summarizes various types of biomarkers, their classification, and monitoring and detection methods and strategies. It also discusses analytical techniques and clinically applicable biomarker sensing techniques, including recent developments in nanotechnology-based biomarker sensing and detection.
Article
Biochemistry & Molecular Biology
Benedetta Carrozzini, Giovanni Luca Cascarano, Carmelo Giacovazzo
Summary: This article introduces a new molecular replacement program, REMO22, which is effective in model searching for proteins and nucleic acids by utilizing various types of prior information. The program has been validated through several test cases and is primarily developed as an automated tool for determining crystal structures of macromolecules. Experimental tests comparing REMO22 with leading MR programs indicate that REMO22, along with its pipeline REMO22 + SYNERGY + CAB, presents a viable alternative to currently used phasing tools.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Peng Gao, Ruyue Wei, Yuanyuan Chen, Xiaohan Liu, Jie Zhang, Wei Pan, Na Li, Bo Tang
Summary: A tricolor fluorescent nanoprobe based on COF was developed for simultaneous imaging of biomarkers in living cells, enabling cancer cell-specific diagnostic imaging and monitoring of cancer cell exosomes infecting normal cells. This versatile nanoprobe offers new insights for developing novel COF-based nanoprobes.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Lei Jiao, Weiqing Xu, Yu Wu, Hengjia Wang, Liuyong Hu, Wenling Gu, Chengzhou Zhu
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Rongbing Li, Xiaoxuan Sun, Yujia Jiang, Ling Liu, Yunhui Li, Dengbin Yu, Jianwei Zhu, Shaojun Dong
Summary: In this study, a colorimetric biosensor based on the rapid color change of E. coli and BQ was introduced for water toxicity evaluation. The biosensor showed a dose-dependent response, with IC20 values for Cu2+ and Hg2+ obtained based on RGB values. It has the potential to quickly detect the toxicity of Cu2+ and Hg2+ in water.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Physical
Jianguo Zhao, He Zhang, Xiaoxuan Sun, Shuai Hao, Panpan Zhao, Xinyang Zhu, Shaojun Dong
Summary: This paper reports an integrated bio-photoelectrochemical system based on photo-enzyme coupled catalysis for glucose oxidation and hydrogen peroxide production. The proposed system shows excellent performance in terms of maximum power output and efficient H2O2 accumulation, expanding the functional versatility of green energy devices.
JOURNAL OF MATERIALS CHEMISTRY A
(2023)
Article
Chemistry, Analytical
Ruimin Li, Weiwei Guo, Zhijun Zhu, Yanling Zhai, Guanwen Wang, Zheng Liu, Lei Jiao, Chengzhou Zhu, Xiaoquan Lu
Summary: A single-atom indium anchored on nitrogen-doped carbon (In1-N-C) catalyst was prepared for sensitive electrochemical sensing of dopamine (DA). The In1-N-C catalyst exhibited high catalytic performance for DA oxidation, showing enhanced intrinsic activity with atomically dispersed In-N4 sites. The concept application of an electrochemical sensor for DA detection with high sensitivity and selectivity was established.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Zhixuan Yu, Jiaxin Zhang, Jinxing Chen, Liyi Zhao, Dengbin Yu, Ling Liu, Shaojun Dong
Summary: fi-D-Glucuronidase (GUS) plays a crucial role in clinical treatment assessment and environmental monitoring. However, current GUS detection tools have limitations in terms of pH continuity and diffusion. In this study, a novel GUS recognition tool called ERNathG was developed, which addressed these issues by utilizing pH-matching and endoplasmic reticulum-anchoring strategies. ERNathG enabled continuous and anchored GUS detection without pH adjustment, demonstrating superior properties compared to commonly used commercial molecules.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jinli Li, Hengjia Wang, Mingwang Liu, Ying Qin, Qie Fang, Rong Tan, Liuyong Hu, Wenling Gu, Chengzhou Zhu
Summary: Photoelectrochemical enzymatic biosensors have attracted attention due to their specificity and sensitivity, but the charge migration between enzymes and semiconductors is uncertain. This study successfully immobilized horseradish peroxidase (HRP) on ionic liquid-modified Cu@Cu2O aerogels to enhance charge transfer and interfacial redox reactions. The improved catalytic ability of HRP after immobilization was demonstrated through enhanced redox ability under light irradiation. By utilizing the excellent PEC activity and catalysis reaction of IL-Cu@Cu2O@HRP, an immunoassay platform was constructed for sensing prostate-specific antigens with a wide detection range and low limit of detection. A new perspective for designing advanced PEC sensing platforms is provided through a better understanding of direct electronic communication between photoactive materials and enzymes for boosted charge transfer and interfacial catalysis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Weiqing Xu, Yu Wu, Xiaosi Wang, Ying Qin, Hengjia Wang, Zhen Luo, Jing Wen, Liuyong Hu, Wenling Gu, Chengzhou Zhu
Summary: In this study, enzyme-inspired Fe-based single-atom catalysts were used to generate specific reactive oxygen species (ROS) for cathodic/anodic electrochemiluminescence (ECL) conversion. Mechanistic studies revealed that the FeN5 sites produced highly active hydroxyl radicals, enabling direct cathodic luminescence, while the FeN4 (c) sites produced superoxide radicals, resulting in inefficient anodic ECL. Based on the enhanced cathodic ECL, an FeN5 SAC-based immunosensor was developed for sensitive detection of cancer biomarkers.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Long Pang, Zhiwei Zhao, Xian-Yin Ma, Wen-Bin Cai, Limin Guo, Shaojun Dong, Chuntai Liu, Zhangquan Peng
Summary: Researchers have developed a novel analytical method that combines differential electrochemical mass spectrometry and attenuated total reflection surface enhanced infrared absorption spectroscopy to simultaneously detect deposited and volatile interface species. This technique is particularly suitable for non-aqueous, electrolyte-based energy devices. By applying this method in lithium-ion and lithium-oxygen batteries, they obtained valuable reaction mechanisms. Overall, this coupled and complementary technique provides important insights into the interfacial electrochemistry of energy storage materials and is expected to generate significant interest in the electrochemistry community and beyond.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Chemistry, Physical
Panpan Zhao, Xiaoxuan Sun, Shuai Hao, Yuehan Zhang, Jinxing Chen, He Zhang, Shaojun Dong
Summary: In this work, rhodium single-atom nanozymes (Rh SANs) were designed to mimic the function of natural glucose oxidase (GOD) for glucose oxidation. The Rh SANs demonstrated the same biocatalytic mechanism and electrocatalytic path as natural GOD. By coupling a bilirubin oxidase biocathode, a Rh SANs-based glucose/O2 enzymatic biofuel cell (EBFC) was developed with a significantly enhanced power density compared to a natural GOD-based EBFC. This device not only accelerates the development of nanozymes but also paves avenues for powering portable and wearable devices.
ACS ENERGY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Rong Tan, Ying Qin, Mingwang Liu, Hengjia Wang, Rina Su, Runshi Xiao, Jinli Li, Liuyong Hu, Wenling Gu, Chengzhou Zhu
Summary: Semiconductor-based photoelectrochemical (PEC) fuel cells convert solar and chemical energy into electrical energy, offering a sustainable and environmentally friendly energy production solution. However, their low PEC activities due to electron-hole recombination and slow reaction kinetics limit their practical application. To overcome this, a unique PEC fuel cell composed of dual photoelectrodes utilizing low-cost biomass as an organic fuel is reported. The integration of bifunctional iron single-atom catalysts (Fe SACs) and photoactive materials effectively enhances charge carrier transfer and boosts reaction kinetics and conversion efficiency.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Analytical
Zhen Luo, Weiqing Xu, Zhichao Wu, Lei Jiao, Xin Luo, Mengzhen Xi, Rina Su, Liuyong Hu, Wenling Gu, Chengzhou Zhu
Summary: The Ru(bpy)(3)(2+)-tripropylamine ECL system is widely used in commercial bioanalysis, but the presence of amines in the biological environment causes unwanted anodic interference signals. To overcome this, a cathodic Ru(bpy)(3)(2+) ECL system is proposed, which utilizes the iron-nitrogen-carbon catalyst to activate the ECL signal. This innovative system shows great potential for practical applications, demonstrated by the successful development of an ECL biosensor for detecting alkaline phosphatase activity.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xin Luo, Zhen Luo, Shentian Li, Qie Fang, Weiqing Xu, Hengjia Wang, Yongze Wang, Guang-Ming Bao, Wenling Gu, Chengzhou Zhu
Summary: Self-powered sensing system (SPSS) integrating nanozymatic biofuel cell enables sensitive detection of prostate-specific antigen (PSA) with a wide detection range and low detection limit. The nanozymatic biofuel cell exhibits higher power output and stability compared to the Pt/C-based counterpart, making it beneficial for the application of SPSS and broadening the application scenarios of nanozymes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Weiqing Xu, Yu Wu, Yuling Xu, Xiaoli Cai, Wenling Gu, Chengzhou Zhu
Summary: This study presents a spatially compartmentalized artificial organelle for correcting intratumoral metabolite analysis. The organelle consists of a zeolitic imidazolate framework-8 immobilized glucose oxidase-horseradish peroxidase cascade core and catalase-directed shell, which function as signal transduction and guarding rooms, respectively. The core and shell provide appropriate spaces to enhance biocatalytic efficiency and tolerate complex tumor microenvironments. Moreover, catalase decomposes endogenous H2O2 to O-2, overcoming interference in signal output and alleviating hypoxic states, thus maximizing glucose oxidation. The artificial organelle demonstrates great potential for accurate intratumoral metabolite analysis in vitro and in vivo.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Chemistry, Multidisciplinary
Weiqing Xu, Yu Wu, Wenling Gu, Dan Du, Yuehe Lin, Chengzhou Zhu
Summary: Metal-organic frameworks (MOFs) have made significant advances as mimics of metalloenzymes in bioinspired catalysis, particularly in the field of active pocket engineering at the atomic level.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Na Tao, Lei Jiao, Huihuang Li, Liu Deng, Wei Wang, Senfeng Zhao, Wansong Chen, Limiao Chen, Chengzhou Zhu, You-Nian Liu
Summary: This study demonstrates a mild hyperthermia-enhanced pyroptosis-mediated immunotherapy based on hollow carbon nanozyme, which can amplify anticancer responses in the tumor microenvironment and has implications for clinical immunotherapy.
