Journal
ELECTROANALYSIS
Volume 22, Issue 20, Pages 2389-2398Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200900634
Keywords
Biosensors; Nitrite reductase; Viologen; Polysiloxane; Hydrophilic polyuretane
Categories
Funding
- Ministry of Education, Science and Technology [M10536090001-05N3609-00110]
- Ministry of Knowledge Economy (MKE)
- KOSEF [R01-2008-000-20704-0]
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Copper containing nitrite reductase (Cu-NiR) and viologen-modified sulfonated polyaminopropylsiloxane (PAPS-SO3H-V) were co-immobilized on glassy carbon electrode (GCE) by hydrophilic polyurethane (HPU) drop-coating, and the electrode was tested as a reagentless electrochemical biosensor for nitrite detection. The newly synthesized PAPS-SO3H-V as an electron transfer (ET) mediator between electrode and NiR was effective, and could be effectively immobilized in HPU membrane. The NiR and PAPS-SO3H-V co-immobilized GCE used as a nitrite biosensor showed the following performance factors: sensitivity = 12.0 nA mu M-1, limit of detection (LOD) = 60 nM (S/N = 3), linear response range = 0-18 mu M (r(2) = 0.996) and response time (t(90%)) = 60 s, respectively. Lineweaver-Burk plot shows that apparent Michaelis-Menten constant (K-M(app)) is 101 mu M. Storage stability of the sensor is 51 days (80% of initial activity) in condition of storing in ambient air at room temperature. The sensor showed a relative standard deviation (RSD) of 3.2% (n = 5) even in condition of injection of 1 mu M nitrite. Interference study showed that common anions in water sample such as chlorate, chloride, sulfate and sulfite do not interfere with the nitrite detection. However, nitrate interfered with a relative sensitivity of 80% due to inherent character of the enzyme used.
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