Journal
ELECTROANALYSIS
Volume 21, Issue 14, Pages 1630-1635Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200804580
Keywords
Luminescence; Luminol; Sensitization; Reactive oxygen species; Neutral medium; Bioorganic chemistry
Categories
Funding
- National Natural Science Foundation of China [20275025, 20675055]
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In this paper, the sensitization on electrochemiluminescent (ECL) reaction of luminol from reactive oxygen species (ROSs) in neutral medium was studied. The powerful sensitization from ROSs even related with organics and organisms were examined under selected conditions which were suitable for biochemical analysis. The results indicated that whether the enhancers were dissolved in solutions or immobilized on the surface of conventional electrodes, stronger ECL intensity of luminol could be obtained. Enhanced ECL helped to provide groundwork for the detection of biomolecules for which would further enhance or quench the ECL signals. The technique may provide new means in a variety of fields such as clinical diagnostics, immunological analysis and environmental monitoring due to its simplicity and high efficiency.
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