Article
Chemistry, Analytical
Ning Xia, Gang Liu, Xinyao Yi
Summary: This study presented a heterogeneous surface plasmon resonance (SPR) method for protease detection by integrating homogeneous reaction, with enhanced sensitivity through signal amplification of streptavidin (SA)-conjugated immunoglobulin G (SA-IgG).
Article
Chemistry, Analytical
Yong Chang, Xiaohua Ma, Ting Sun, Lin Liu, Yuanqiang Hao
Summary: A novel heterogeneous electrochemical method for kinase detection was proposed in this work, which does not require immobilization of the probe during phosphorylation. The method captures the biotinylated phosphopeptide on a neutravidin-modified electrode and recognizes phosphate groups using conjugates of biotinylated Phos-tag and ferrocene-capped gold nanoparticles. This strategy is simple, sensitive, and specific, integrating the advantages of homogeneous reaction and heterogeneous detection.
Article
Chemistry, Analytical
Lin Liu, Yong Chang, Xingyue Ji, Jiayu Chen, Mengyu Zhang, Suling Yang
Summary: A sensitive electrochemical biosensor for telomerase detection was developed by integrating homogeneous extension and hybridization reactions and surface-tethered detection. The biosensor exhibited strong electrochemical signals from the oxidation of Fc tags when the bio-DNA/DNA/Fc-AuNP hybrids were tethered to streptavidin-modified electrodes through specific avidin-biotin interactions. This method has great potential in clinical diagnosis and anti-cancer drug development.
Article
Engineering, Electrical & Electronic
Nirgaman Bage, Pradip Kar
Summary: The study investigated the influence of silver nanoparticles labeled with biomolecules on electrochemical sensing, showing that AgNP labeled layers can serve as ultrasensitive detectors for detecting biotin-avidin couple and sensing concentration changes even at higher concentrations.
IEEE SENSORS JOURNAL
(2021)
Article
Chemistry, Analytical
Hema Bhardwaj, Gajjala Sumana, Christophe A. Marquette
Summary: This study reports the development of an electrical and optical biosensor using gold nanobipyramids for label-free detection of Aflatoxin B-1. The sensor showed high sensitivity and robustness, and was successfully applied in detecting AFB-1 in maize samples.
Article
Chemistry, Analytical
Jinling Fu, Jie Wu, Rui Zhang, Qiang Wu, Huangxian Ju
Summary: A universal and highly sensitive electrochemical biosensing strategy utilizing a triplet nanostructure-mediated dendritic hybridization chain reaction (HCR) was developed for the detection of Dengue virus (DENV) nucleic acid, achieving ultrasensitive electrochemical detection with the ability to distinguish single-base mutation. The stable three-dimensional dendritic nanostructure with rigid double helix ensured the excellent performance of the designed electrochemical biosensing strategy. The proposed method showed good extendability for other nucleic acids, providing a promising candidate for nucleic acid detection in early clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Nirgaman Bage, Pradip Kar
Summary: The gold electrode was modified with cysteamine and silver nanoparticles, allowing for the covalent bonding of biotin or avidin. By investigating the changes in interfacial properties and sensing mechanism using cyclic voltammetry and electrochemical impedance spectroscopy, the study demonstrated that the charge transfer resistance was directly proportional to analyte concentration.
INSTRUMENTATION SCIENCE & TECHNOLOGY
(2021)
Article
Polymer Science
Mohd Hafiz Abu Bakar, Nur Hidayah Azeman, Nadhratun Naiim Mobarak, Nur Afifah Ahmad Nazri, Tengku Hasnan Tengku Abdul Aziz, Ahmad Rifqi Md Zain, Norhana Arsad, Ahmad Ashrif A. Bakar
Summary: This research investigates the physicochemical properties of biopolymer succinyl-kappa-carrageenan as a potential sensing material for NH4+ Localized Surface Plasmon Resonance (LSPR) sensor. The results show that AgNP-Succinyl-kappa-carrageenan LSPR has higher performance than AgNP-kappa-carrageenan LSPR, broader detection range than the conventional method, and high selectivity toward NH4+. The adsorption of NH4+ on kappa-carrageenan and succinyl-kappa-carrageenan were through multilayer and chemisorption process that follows Freundlich and pseudo-second-order kinetic model.
Article
Biochemical Research Methods
Xiaoyong Chen, Pin Xu, Wenwei Lin, Jin Jiang, Hang Qu, Xuehao Hu, Jinghua Sun, Yukun Cui
Summary: This study proposes a plasmonic titled fiber Bragg grating biosensor for the detection of breast cancer cells. Experimental results demonstrate that the biosensor has good detection performance, capable of detecting low concentrations of breast cancer cells in a short period of time. The sensor has a simple structure, easy manufacturing process, making it a promising choice for future cancer cell detection.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Engineering, Electrical & Electronic
Francisco J. da Silva, Leiva C. Oliveira, Antonio M. N. Lima
Summary: This article presents the design of an automated platform for manipulating components required for a surface plasmon resonance (SPR) sensing instrument, ensuring quick and accurate operation through alignment and positioning adjustments. The platform's mechanical chassis is constructed with specialized materials, functioning similarly to a manually adjusted test platform.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2021)
Article
Chemistry, Analytical
Lin Liu, Daohong Wu, Shuang Zhen, Kaiqing Lu, Xinyao Yi, Zhifang Sun
Summary: This study presents a simple and sensitive electrochemical strategy for detecting telomerase extracted from HeLa cells, utilizing the signal amplification of in-situ formed streptavidin-biotin-DNA-biotin networks. The method effectively amplifies the signal by creating an insulating layer on a gold electrode surface, thus enabling the detection of telomerase.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Shimeng Chen, Yun Liu, Qingxu Yu, Wei Peng
Summary: A novel SPR biosensing approach with low LOD, high sensitivity, and extensive applicability was introduced in this study. By optimizing the SPR sensor structure and employing a self-referencing sensing strategy, the low LOD of 0.2 fM was achieved.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Physics, Applied
Weiqi He, Weina Zhang, Jiantian Zhang, Peng Yu, Pu Liu, Guowei Yang, Hongxiang Lei
Summary: Utilizing flexible dielectric material polylactic acid (PLA) doped with high refractive index quantum dots (QDs) as the substrate, a nanowire RI sensor capable of high sensitivity and the highest FOM under white light source is fabricated, showing great potential for biological applications.
APPLIED PHYSICS LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Nirgaman Bage, Sadhana Kundu, Arpita Adhikari, Pradip Kar
Summary: By modifying the gold electrode with L-cysteine and coupling biomolecules and functionalized silver nanoparticles, the electrochemical properties are improved. The avidin modified electrode shows better sensing performance for biotin compared to the other method.
RESULTS IN CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Amna Yaqub, Syeda Rubina Gilani, Sehrish Bilal, Akhtar Hayat, Anila Asif, Saadat Anwar Siddique
Summary: A novel redox-sensing film was successfully constructed based on cobalt-substituted polyoxometalate and silver nanoparticles using layer-by-layer self-assembly method, demonstrating excellent electrocatalytic efficiency for carbofuran detection. The fabricated sensor showed high sensitivity and a low detection limit for the highly toxic insecticide.
Article
Materials Science, Multidisciplinary
Xiaoli Cai, Lei Jiao, Hongye Yan, Yu Wu, Wenling Gu, Dan Du, Yuehe Lin, Chengzhou Zhu
Summary: Nanozymes, as substitutes for enzymes, possess enzymelike activities with high stability and tolerance, making them ideal for biomimetic catalysis systems in biomedical applications.
Review
Materials Science, Multidisciplinary
Shichao Ding, Nan Zhang, Zhaoyuan Lyu, Wenlei Zhu, Yu-Chung Chang, Xiaoling Hu, Dan Du, Yuehe Lin
Summary: Protein-based nanomaterials and nanosystems (PNNS) have captured significant scientific interest in recent years due to their potential in bio-applications, with a focus on designing multi-functional PNNS based on different protein characteristics. The latest developments in unique synthesis strategies and detailed classifications of PNNS have been reviewed, highlighting the functions of proteins in biomedical applications such as targeting, carriers, enzymes, and fluorescent indicators. Challenges and future prospects in PNNS research have also been discussed.
Article
Chemistry, Multidisciplinary
Shichao Ding, Zhaoyuan Lyu, Hong Zhong, Dong Liu, Erik Sarnello, Lingzhe Fang, Mingjie Xu, Mark H. Engelhard, Hangyu Tian, Tao Li, Xiaoqing Pan, Scott P. Beckman, Shuo Feng, Dan Du, Jin-Cheng Li, Minhua Shao, Yuehe Lin
Summary: A method for synthesizing carbon-based single-atom metal catalysts using an ion-imprinting derived strategy was proposed to obtain materials enriched with single-atom metal active sites, resulting in outstanding performance in the oxygen reduction reaction.
Article
Chemistry, Multidisciplinary
Yang Song, Xiaoli Cai, Grayson Ostermeyer, Jierui Yu, Dan Du, Yuehe Lin
Summary: Paper-based rapid diagnostic tests are valuable alternatives for biomarker detection, but require further refinement. The integration of self-assembling allochroic nanocatalysts can improve the analytic sensitivity of these tests. The successful utilization of SANs in LFA-based biomarker detection may inspire integration of similar nanocatalysts into immunochromatographic testing methods.
Article
Multidisciplinary Sciences
Xiaoli Cai, Mingming Wang, Peng Mu, Tengyue Jian, Dong Liu, Shichao Ding, Yanan Luo, Dan Du, Yang Song, Chun-Long Chen, Yuehe Lin
Summary: The combination of chemotherapy, photothermal therapy (PTT), and photodynamic therapy (PDT) has shown promising results in treating malignant glioma, one of the most aggressive brain tumors, by achieving high tumor killing efficacy. The synthesized IR780 dye-labeled tube-forming peptoids (PepIR) self-assembled into crystalline nanotubes, showing excellent efficacy for PDT/PTT by effectively packing and separating photosensitizers within nanotubes. Additionally, efficient DOX loading in the nanotubes contributed to synergistic chemotherapy against glioma cells. This multimodal therapeutic system offers great promises for future glioma therapy in clinic.
Review
Chemistry, Multidisciplinary
Mansour Mahmoudpour, Shichao Ding, Zhaoyuan Lyu, Ghasem Ebrahimi, Dan Du, Jafar Ezzati Nazhad Dolatabadi, Mohammadali Torbati, Yuehe Lin
Summary: This review discusses the design and applications of aptamer functionalized nanomaterials (AFMs) in the biomedical field, including synthesis strategies, versatile roles of nanomaterials, and representative examples of biomedical applications. Challenges and opportunities in this emerging field are also presented.
Article
Chemistry, Multidisciplinary
Shichao Ding, Zhaoyuan Lyu, Lingzhe Fang, Tao Li, Wenlei Zhu, Suiqiong Li, Xin Li, Jin-Cheng Li, Dan Du, Yuehe Lin
Summary: Developing novel substitutes for heme enzymes, such as Fe-N-C based single-atomic site catalysts (SASCs), can provide high catalytic activity and selectivity in biosensing applications. Synthesizing a SASC (Fe-SASC/NW) by doping single iron atoms into carbon nanowires shows promising heme enzyme-like catalytic performance for hydrogen peroxide detection. The electrochemical sensor based on Fe-SASC/NW exhibits excellent detection capabilities for H2O2 due to the isolated Fe-N-x active sites and their structural similarity with natural metalloproteases.
Article
Nanoscience & Nanotechnology
Chenxi Huyan, Shichao Ding, Zhaoyuan Lyu, Mark H. Engelhard, Yuhao Tian, Dan Du, Dong Liu, Yuehe Lin
Summary: A new nanostructured ion exchanger PPy@Fe2O3 NN-CC has been designed and synthesized in this study, which effectively enhances the adsorption speed and efficiency of PFBA, ensuring high adsorption capacity and rapid regeneration property, thereby reducing the generation of secondary waste. ESIX based on this nano composite material shows high selectivity for the adsorption of PFBA over other common anions in water.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Yuting Zhao, Xiaofan Ruan, Yang Song, Jordan N. Smith, Natalia Vasylieva, Bruce D. Hammock, Yuehe Lin, Dan Du
Summary: Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-MS (LC-MS) are commonly used methods to detect pesticides, but not suitable for on-site biomonitoring. A smartphone-based dual-channel immunochromatographic test strip (ICTS) was developed for simultaneous detection of cypermethrin and its metabolite, promising for accurate point-of-care biomonitoring of pesticide exposure. Polymer carbon dots (PCDs) were used as signal amplifier, showing excellent linear responses for detecting cypermethrin and 3-phenoxybenzoic acid. This innovative device provides a comprehensive analysis of pesticide exposure in a convenient and efficient manner.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Shichao Ding, Zhaoyuan Lyu, Suiqiong Li, Xiaofan Ruan, Mingen Fei, Yang Zhou, Xiangheng Niu, Wenlei Zhu, Dan Du, Yuehe Lin
Summary: An electrochemical sensor based on molecularly imprinted polypyrrole nanotubes has been developed for the detection of glyphosate with high sensitivity and specificity. The sensor demonstrates a wide detection range, low limit of detection, excellent stability, reproducibility, and selectivity against interferents. The sensor's practicality has been verified by detecting glyphosate in orange juice and rice beverages, making it suitable for monitoring pesticides in actual food and environmental samples.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Yang Song, P. Grayson Ostermeyer, Dan Du, Yuehe Lin
Summary: Carbon dot-encapsulated silica nanospheres (CSNs) exhibit good material stability and signal amplification, making them suitable for use in solid-phase immunoassays. Through E. coli O157:H7 quantitation experiments, the detection limit was determined to be 2.4 CFU mL(-1), with estimated recoveries in the range of 91.7-110.5% in spiked samples, indicating the method's capability for detecting E. coli O157:H7 in food samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Physical
Shichao Ding, Zhaoyuan Lyu, Erik Sarnello, Mingjie Xu, Lingzhe Fang, Hangyu Tian, Sam Ellery Karcher, Tao Li, Xiaoqing Pan, John McCloy, Guodong Ding, Qiang Zhang, Qiurong Shi, Dan Du, Jin-Cheng Li, Xiao Zhang, Yuehe Lin
Summary: In this study, a MnOx engineered Fe-N-C single-atom catalyst (Mn-Fe-N-C SAC) was developed to reduce oxidative corrosion and enhance the stability of the oxygen reduction reaction (ORR). The Mn-Fe-N-C SAC exhibited ultralow H2O2 yield and negligible potential shift after continuous potential cycles, demonstrating excellent ORR stability and improved activity compared to common Fe-N-C SACs. The interaction between MnOx and Fe-N-x sites enhanced the intrinsic activity of single iron sites, providing a promising solution for the stability issue of Fe-N-C SACs while maintaining high catalytic activity.
JOURNAL OF MATERIALS CHEMISTRY A
(2022)
Article
Environmental Sciences
Yuhao Tian, Jianyu Xing, Chenxi Huyan, Chengzhou Zhu, Dan Du, Wenlei Zhu, Yuehe Lin, Indranil Chowdhury
Summary: A novel process for PFOA removal from contaminated water was developed using electrically switched ion exchange with PPy deposited on conductive materials. The PPy showed excellent selectivity and regeneration ability in batch experiments, providing a promising method for addressing PFOA contamination in natural aqueous environments.
Article
Materials Science, Multidisciplinary
Yang Song, Mingming Wang, Susrut Akkineni, Wenchao Yang, Jeevapani J. Hettige, Haibao Jin, Zhihao Liao, Peng Mu, Feng Yan, Marcel Baer, James J. De Yoreo, Dan Du, Yuehe Lin, Chun-Long Chen
Summary: In this study, highly bright and photostable membrane-mimetic 2D nanosheets were designed and synthesized from sequence-defined peptoids, showing high quantum yield and photostability due to the precise placement of dye molecules. These nanosheets were utilized as biocompatible and programmable probes for live cell imaging and cell labeling, with the potential for enhanced lysosome escape and efficient intracellular delivery of macromolecular drugs by programming different surface charges.
ACS MATERIALS LETTERS
(2021)
Review
Chemistry, Multidisciplinary
Lei Jiao, Weiqing Xu, Yu Wu, Hongye Yan, Wenling Gu, Dan Du, Yuehe Lin, Chengzhou Zhu
Summary: The development of highly sensitive biosensors has been a focus in recent years. Single-atom catalysts show promise in enhancing signal amplification in biosensing, offering improved sensitivity and selectivity through tunable metal-support interactions and the structural features of active sites.
CHEMICAL SOCIETY REVIEWS
(2021)