Journal
ELECTROANALYSIS
Volume 20, Issue 11, Pages 1220-1226Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200704169
Keywords
DNA biosensor; multiwalled carbon nanotubes; gold nanoparticles; layer-by-layer assembly; covalent attachment
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A novel DNA biosensor has been fabricated for the detection of DNA hybridization based on layer-by-layer (LBL) covalent assembly of gold nanoparticles (GNPs) and multiwalled carbon nanotubes (MWCNTs). The stepwise LBL assembly process was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The hybridization events were monitored by differential pulse voltammetry (DPV) measurement of the intercalated doxorubicin, and the factors influencing the performance of the DNA hybridization was investigated in detail. ne signal was linearly changed with target DNA concentration increased from 0.5 to 0.01 nM, and had a detection limit of 7.5 pM (signal/noise ratio of 3). In addition, the DNA biosensor showed an excellent reproducibility and stability under the DNA-hybridization conditions.
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