Journal
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 107, Issue -, Pages 214-219Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2014.06.006
Keywords
Toxicity; Antioxidative system; GST; MDA; ROS; SCGE
Categories
Funding
- National Natural Science Foundation of China [21277083, 41071164, 21377075, 41001152]
- Specialized Research Fund for the Doctoral Program of Higher Education [20113702110007]
- Higher Educational Science and Technology Program in Shandong Province [J12LC01]
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Azoxystrobin has been widely used in recent years. The present study investigated the oxidative stress and DNA damage effects of azoxystrobin on earthworms (Eisenia fetida). Earthworms were exposed to different azoxystrobin concentrations in an artificial soil (0, 0.1, 1, and 10 mg/kg) and sampled on days 7, 14, 21, and 28. Superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POD), glutathione-Stransferase (GST), reactive oxygen species (ROS), and malondialdehyde (MDA) content were measured by an ultraviolet spectrophotometer to determine the antioxidant responses and lipid peroxidation. Single cell gel electrophoresis (SCGE) was used to detect DNA damage in the coelomocytes. Compared with these in the controls, earthworms exposed to azoxystrobin had excess ROS accumulation and greater SOD, POD, and GST activity while the opposite trend occurred for CAT activity. MDA content increased after 14-day exposure, and DNA damage was enhanced with an increase in the concentration of azoxystrobin. In conclusion, azoxystrobin caused oxidative stress leading to lipid peroxidation and DNA damage in earthworms. (C) 2014 Elsevier Inc. All rights reserved.
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