Journal
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 73, Issue 7, Pages 1666-1670Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2010.07.013
Keywords
Acetylcholinesterase mRNA; Common carp; Real-time quantitative PCR; Chlorpyrifos; Atrazine
Categories
Funding
- Governmental Public Industry Research Special Funds [200909043]
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The uses of pesticides and herbicides have become an integral part of modern agricultural systems. The intensive use of pesticides chlorpyrifos (CPF) and herbicides atrazine (AIR) has resulted in serious environmental problems. Herein, we have developed real-time quantitative polymerase chain reaction assays for common carp (Cyprinus carpio L.) mRNA. The levels of AChE mRNA were evaluated in brain and muscle collected from common carp by treatment of ATR, CPF, and their mixture. The decreased transcription of AChE was detected in both tissues at different doses of the toxicants in the end of exposure tests, and the changes were improved in the end of recovery tests in varying degrees. It is suggested that transcription inhibition of AChE might be significant in long-playing single or associated exposure of AIR and CPF in common carp. Alteration in transcription of AChE caused by ATR, CPF, and their mixture could reveal the toxic mechanisms related to cholinergic signaling. (C) 2010 Elsevier Inc. All rights reserved.
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