Journal
ECOTOXICOLOGY
Volume 21, Issue 5, Pages 1381-1390Publisher
SPRINGER
DOI: 10.1007/s10646-012-0892-5
Keywords
Roundup (R); Glyphosate; POEA; Genotoxicity; DNA lesion-specific repair enzymes; Fish
Categories
Funding
- Fundacao para a Ciencia e Tecnologia (FCT
- Government of Portugal) [PTDC/AAC-AMB/114123/2009]
- FCT/MCTES in its national budget component (PIDDAC)
- European Regional Development Fund (ERDF) through COMPETE - Thematic Factors of Competitiveness Operational Programme (POFC)
- Ph.D. fellowship [SFRH/BD/42103/2007]
- Centre for Environmental and Marine Studies (CESAM)
- Fundação para a Ciência e a Tecnologia [PTDC/AAC-AMB/114123/2009] Funding Source: FCT
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It has been widely recognized that pesticides represent a potential threat in aquatic ecosystems. However, the knowledge on the genotoxicity of pesticides to fish is still limited. Moreover, genotoxic studies have been almost exclusively focused on the active ingredients, whereas the effect of adjuvants is frequently ignored. Hence, the present study addressed the herbicide Roundup(A (R)), evaluating the relative contribution of the active ingredient (glyphosate) and the surfactant (polyethoxylated amine; POEA) to the genotoxicity of the commercial formulation on Anguilla anguilla. Fish were exposed to equivalent concentrations of Roundup(A (R)) (58, 116 mu g L-1), glyphosate (17.9, 35.7 mu g L-1) and POEA (9.3, 18.6 mu g L-1), during 1 and 3 days. The comet assay was applied to blood cells, either as the standard procedure, or with an extra step involving DNA lesion-specific repair enzymes in an attempt to clarify DNA damaging mechanisms. The results confirmed the genotoxicity of Roundup(A (R)), also demonstrating the genotoxic potential of glyphosate and POEA individually. Though both components contributed to the overall genotoxicity of the pesticide formulation, the sum of their individual effects was never observed, pointing out an antagonistic interaction. Although POEA is far from being considered biologically inert, it did not increase the risk associated to glyphosate when the two were combined. The analysis of oxidatively induced breaks suggested that oxidation of DNA bases was not a dominant mechanism of damage. The present findings highlighted the risk posed to fish populations by the assessed chemicals, jointly or individually, emphasizing the need to define regulatory thresholds for all the formulation components and recommending, in particular, the revision of the hazard classification of POEA.
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