Journal
E-JOURNAL OF CHEMISTRY
Volume 7, Issue -, Pages S239-S244Publisher
HINDAWI LTD
DOI: 10.1155/2010/724915
Keywords
Gemcitabine HCl; Lyophilized injection; Forced degradation study; HPLC
Categories
Ask authors/readers for more resources
A stability indicating RP HPLC method has been developed for the determination of gemcitabine hydrochloride. Chromatography was carried out on an ODS C-18 column (250x4.6 mm; 5 mu) using a mixture of methanol and phosphate buffer (40: 60 v/v) as the mobile phase at a flow rate of 1.0 mL/min. The detection of the drug was monitored at 270 nm. The retention time of the drug was found to be 2.31 min. The method produced linear responses in the concentration range of 10 to 60 mu g/mL of gemcitabine HCl. The method was found to be reproducible for analysis of the drug in injectable dosage forms. The stability of the drug was assessed by forced degradation studies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available