Journal
DRYING TECHNOLOGY
Volume 28, Issue 11, Pages 1245-1254Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/07373937.2010.498068
Keywords
Cercospora kikuchii; Enzyme stabilization; Lipase; Spray drying
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Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP [04/07935-6, 08/52732-7]
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In this study the effects of spray-drying conditions on the retention of enzyme activity of lipase produced by the endophytic fungus Cercospora kikuchii have been investigated. Drying runs were carried out in a bench-top spray dryer with a concurrent flow regime. The influence of the variables inlet temperature of drying gas, Tgi (86.4 to 153.6 degrees C); mass flow rate of the enzymatic extract fed to the dryer, Ws (2.63 to 9.36g/min); and concentration of the drying adjuvant added to the extract, ADJ (1.95 to 12.05%), on the spray-drying performance and on product quality was evaluated through experimental planning and regression analysis. The use of maltodextrin, as a stabilizing agent, slightly improved the retention of enzyme activity compared to -cyclodextrin. Statistical optimization of the experimental results allowed the determination of the processing conditions that maximized the retention of the enzymatic activity (RAE), namely, concentration of drying adjuvants of 12.05%, inlet temperature of the drying gas of 153.6 degrees C, and flow rate of the enzymatic extract fed to the dryer of 9.36g/min for the both drying adjuvants investigated.
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