Article
Microbiology
Habibou Sarr, Aissatou Ahmet Niang, Amadou Diop, Oleg Mediannikov, Hanane Zerrouki, Seydina M. Diene, Seynabou Lo, Mouhamadou Lamine Dia, Ahmad Iyane Sow, Florence Fenollar, Jean-Marc Rolain, Linda Hadjadj, Carla Calia, Lawrence S. Young
Summary: This study investigated the antibiotic resistance of enterobacteria in Senegal and identified the emergence of carbapenemase-producing Enterobacterales (CPE) infections. The results showed that carbapenemase genes and class A β-lactamase genes were widely present in enterobacteria. This phenomenon is concerning, and strict surveillance is necessary to prevent further spread.
Article
Public, Environmental & Occupational Health
Dina Daaboul, Saoussen Oueslati, Mariam Rima, Issmat I. Kassem, Hassan Mallat, Aurelien Birer, Delphine Girlich, Monzer Hamze, Fouad Dabboussi, Marwan Osman, Thierry Naas
Summary: This study investigated the prevalence of carbapenem- and extended-spectrum cephalosporin-resistant Enterobacterales (CR-E and ESCR-E) in Lebanese clinical settings, and found a broad dissemination of these resistant bacteria. The weak awareness of antimicrobial resistance in the Lebanese community, coupled with ongoing economic and healthcare challenges, calls for urgent measures to combat this problem.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Microbiology
Michael R. Jacobs, Caryn E. Good, Ayman M. Abdelhamed, Robert A. Bonomo
Summary: Direct antimicrobial susceptibility testing (AST) of positive blood cultures with Gram-negative bacteria produces results within 24 h, compared to 48 to 96 h with conventional methods. The accuracy of direct AST methods is high, and the application of expert rules improves agreement for beta-lactam agents.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Danieli Conte, Dany Mesa, Thomas Jove, Caetana Paes Zamparette, Thais Cristine Marques Sincero, Jussara Kasuko Palmeiro, Libera Maria Dalla-Costa
Summary: Mobile genetic elements contribute to the emergence and spread of multi-drug-resistant bacteria. This study characterizes the genetic backbone of bla(GES) in Aeromonas spp. and Klebsiella spp. isolated from untreated hospital effluents. The results reveal large genetic diversity in these species and emphasize the importance of considering the public health risk associated with antimicrobial-resistant genes.
MICROBIOLOGY SPECTRUM
(2022)
Review
Infectious Diseases
Oumar Ouchar Mahamat, Marie Kempf, Manon Lounnas, Abelsalam Tidjani, Mallorie Hide, Julio A. Benavides, Christian Carriere, Anne-Laure Banuls, Helene Jean-Pierre, Abdoul-Salam Ouedraogo, Yann Dumont, Sylvain Godreuil
Summary: The study investigated the knowledge gaps on ESBL-E and CPE in West and Central Africa using a 'One Health' approach, revealing major gaps in understanding ESBL and CPE in animals and the environment. The results emphasize the urgent need to improve active surveillance programs in each country and to support antimicrobial stewardship.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Infectious Diseases
Kira Schmitt, Michael Biggel, Roger Stephan, Barbara Willi
Summary: This study investigated the infection prevention and control standards in a companion animal clinic in Switzerland and found major deficiencies in surface disinfection and hand hygiene compliance, resulting in the spread of antibiotic-resistant bacteria. Various antibiotic-resistant bacteria were found on environmental and employee surfaces, and plasmidic dissemination of bla(OXA-48) was observed within the clinic. The study highlights the importance of improving infection prevention and control standards in veterinary clinics to prevent the spread of antibiotic-resistant microorganisms into the community.
Article
Infectious Diseases
Asegdew Atlaw Woldeteklie, Habtamu Biazin Kebede, Abdurezak Ahmed Abdela, Yimtubezinash Woldeamanuel
Summary: This study evaluated the microbiological analysis and drug susceptibility testing of diabetic foot ulcer infections, highlighting the need for improved management.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Environmental Sciences
Guilherme Sgobbi Zagui, Natalia Columbaro Moreira, Danilo Vitorino Santos, Cristina Filomena Pereira Rosa Paschoalato, Jordi Sierra, Marti Nadal, Jose L. Domingo, Ana Lucia Costa Darini, Leonardo Neves Andrade, Susana Ines Segura-Munoz
Summary: In this study, the occurrence of multidrug-resistant Enterobacter spp. in different water sources was investigated. The isolates showed resistance mainly to β-lactam antibiotics and harbored β-lactam antibiotic resistance genes, with blaKPC being widely detected. Metal tolerance genes were also found in the isolates. These findings highlight the urgent need for control measures to prevent the spread of antibiotic-resistant Enterobacter spp. and emphasize the waterborne transmission of clinically important antibiotic-resistant bacteria.
ENVIRONMENTAL RESEARCH
(2023)
Article
Infectious Diseases
Agnes B. Jousset, Sandrine Bernabeu, Cecile Emeraud, Remy A. Bonnin, Alexandra Lomont, Jean Ralph Zahar, Audrey Merens, Christophe Isnard, Nathalie Soismier, Eric Farfour, Vincent Fihman, Nicolas Yin, Olivier Barraud, Herve Jacquier, Anne-Gaelle Ranc, Frederic Laurent, Stephane Corvec, Louise Ruffier d'Epenoux, Emmanuelle Bille, Nicolas Degand, Chloe Plouzeau, Thomas Guillard, Vincent Cattoir, Asaf Mizrahi, Antoine Grillon, Frederic Janvier, Cecile Le Brun, Marlene Amara, Mathilda Bastide, Alban Lemonnier, Laurent Dortet
Summary: This study aimed to provide susceptibility data on a large series of Enterobacterales since the revision of EUCAST categorization break-points in 2020. The results showed that Ceftolozane-tazobactam (C/T) had a susceptibility rate of 88% in Enterobacterales infections, with higher susceptibility in 3GC-resistant strains and lower susceptibility in CRE strains. This prospective study highlights the importance of C/T in the treatment of infections.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Environmental Sciences
Ana Puljko, Svjetlana Dekic Rozman, Ivan Barisic, Ana Maravic, Marko Jelic, Ivana Babic, Milena Milakovic, Ines Petric, Nikolina Udikovic-Kolic
Summary: The study aimed to quantify enteric opportunistic pathogens (EOPs), resistance genes, and isolate enterobacterial strains from hospital wastewater. The results revealed high levels of ESBL and carbapenemase-producing Enterobacterales (CPE) isolates, all of which were multidrug-resistant. Therefore, effective pretreatment of hospital wastewater is necessary to prevent the transmission of clinically important pathogens and resistance genes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Infectious Diseases
Michael Pietsch, Yvonne Pfeifer, Stephan Fuchs, Guido Werner
Summary: Resistance plasmids can be advantageous for bacterial hosts under selective conditions, but may impose fitness costs in the absence of selective pressure. This study investigated the fitness effects of transferring plasmids containing various beta-lactamase genes into Escherichia coli. Results showed that most plasmid acquisitions led to growth differences, with long-term growth experiments revealing compensation of fitness burdens and benefits through potential compensatory mutations identified in the evolved transconjugants' genomes.
Article
Biochemistry & Molecular Biology
Nabi Jomehzadeh, Morteza Saki, Khadijeh Ahmadi, Golshan Zandi
Summary: This study aimed to determine the prevalence of quinolone resistance and the presence of plasmid-mediated quinolone resistance (PMQR) genes among extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates. The results showed a high resistance rate to nalidixic acid and ciprofloxacin among ESBL-producing isolates. The most frequent PMQR gene was aac(6')-Ib-cr variant. The coexistence of aac(6')-Ib-cr variant and qnrB was the most widely distributed genotype among quinolone resistance isolates.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Environmental Sciences
Hasna Said Mohamed, Lokman Galal, Juliette Hayer, Julio A. Benavides, Anne-Laure Banuls, Chloe Dupont, Guilhem Conquet, Christian Carriere, Yann Dumont, Marie-Noelle Didelot, Anne-Laure Michon, Helene Jean-Pierre, Mohamed Houmed Aboubaker, Sylvain Godreuil
Summary: The study provides valuable insights into the epidemiology of carbapenem resistance at the human-animal-environment interface in Djibouti City, East Africa. The prevalence of carbapenemase-producing Gram-negative bacteria (CP-GNB) was relatively low, but plasmids carrying carbapenem resistance genes were found in humans, animals, and water samples. The study highlights the need for preventive and control measures to reduce the circulation of these potentially emerging public health threats.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Infectious Diseases
James Stewart, Louise M. Judd, Adam Jenney, Kathryn E. Holt, Kelly L. Wyres, Jane Hawkey
Summary: This study collected 239 clinical isolates of Klebsiella oxytoca from a hospital and found that they actually encompassed four different species, demonstrating significant diversity. Although antimicrobial resistance was rare, the resistance plasmids were similar to those found in other Enterobacterales, indicating potential for multi-drug resistance.
BMC INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Tsuyoshi Sekizuka, Rina Tanaka, Masanori Hashino, Koji Yatsu, Makoto Kuroda
Summary: This study characterizes environmental antimicrobial resistance (AMR) in urban areas by analyzing wastewater samples from urban wastewater treatment plants in Tokyo. The findings reveal the presence of extended-spectrum beta-lactamase- (ESBL)/carbapenemase-producing bacteria and their genetic characteristics. The study highlights the potential source of AMR burden on public health in urban communities.
Article
Infectious Diseases
Ana Paula Ramalho da Costa-Lourenco, A. Jeanine Abrams, Kesia Thais Barros dos Santos, Isabella Campelo Vilardi Argentino, Talita Coelho-Souza, Maria Cristina Albuquerque Canine, Adriana Lucia Pires Ferreira, Beatriz Meurer Moreira, Sergio Eduardo Longo Fracalanzza, David L. Trees, Raquel Regina Bonelli
INFECTION GENETICS AND EVOLUTION
(2018)
Article
Environmental Sciences
Gabriela Bergiante Kraychete, Eloiza Helena Campana, Renata Cristina Picao, Raquel Regina Bonelli
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Biotechnology & Applied Microbiology
Kesia T. Barros dos Santos, Larissa B. Skaf, Livia H. Justo-da-Silva, Raphael C. Medeiros, Ronaldo da S. Francisco Junior, Maria Cristina A. Canine, Sergio E. L. Fracalanzza, Raquel R. Bonelli
BIOMED RESEARCH INTERNATIONAL
(2019)
Article
Fisheries
A. F. M. Martins, T. L. Pinheiro, A. Imperatori, S. M. Freire, L. Sa-Freire, B. M. Moreira, R. R. Bonelli
Article
Infectious Diseases
Luis G. A. Longo, Viviane S. de Sousa, Gabriela B. Kraychete, Livia H. Justo-da-Silva, Jaqueline A. Rocha, Silvana V. Superti, Raquel R. Bonelli, Ianick S. Martins, Beatriz M. Moreira
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2019)
Article
Infectious Diseases
Larissa Alvarenga Batista Botelho, Gabriela Bergiante Kraychete, Patricia Batista Rocha, Ana Paula de Souza da-Silva, Renata Cristina Picao, Beatriz Meurer Moreira, Raquel Regina Bonelli
MICROBIAL DRUG RESISTANCE
(2020)
Letter
Infectious Diseases
Herrison Fontana, Larissa Brasil Skaf, Ana Filipa Moreira Martins, Lais Lisboa Correa, Beatriz Meurer Moreira, Raquel Regina Bonelli
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2019)
Review
Agriculture, Dairy & Animal Science
Renata F. Rabello, Raquel R. Bonelli, Bruno A. Penna, Julia P. Albuquerque, Rossiane M. Souza, Aloysio M. F. Cerqueira
Article
Engineering, Environmental
Stella Maria Casas Novas Mancano, Eloiza Helena Campana, Thais Pessanha Felix, Lina Rachel Leite Barrueto, Polyana Silva Pereira, Renata Cristina Picao
Article
Microbiology
Felipe Miceli Farias, Lucia Martins Teixeira, Deyse Christina Vallim, Maria do Carmo de Freire Bastos, Marco Antonio Lemos Miguel, Raquel Regina Bonelli
Summary: This study investigated the genetic and phenotypic characteristics of BLIS produced by Enterococcus faecium E86, focusing on the structural genes of two identified bacteriocins. Despite E. faecium E86 carrying two bacteriocinogenic gene clusters, only one cluster was found to encode a functional bacteriocin, enterocin P, which showed strong antibacterial activity.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Microbiology
Bruna Fuga, Fabio P. Sellera, Louise Cerdeira, Fernanda Esposito, Brenda Cardoso, Herrison Fontana, Quezia Moura, Adriana Cardenas-Arias, Elder Sano, Rosineide M. Ribas, Albalucia C. Carvalho, Maria Cristina B. Tognim, Marcia Maria C. de Morais, Ana Judith P. G. Quaresma, Angela Patricia Santana, Joice N. Reis, Marcelo Pilonetto, Eliana Carolina Vespero, Raquel R. Bonelli, Aloysio M. F. Cerqueira, Thais C. M. Sincero, Nilton Lincopan
Summary: The rapid spread of carbapenem and third-generation cephalosporin-resistant pathogens in Brazil poses a serious threat to global health, emphasizing the importance of integrated whole-genome sequencing surveillance data from human, animal, and environmental sources in tracking transmission routes and developing effective prevention and control strategies.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Ana Paula de Souza da-Silva, Viviane Santos de Sousa, Luis Guilherme de Araujo Longo, Saharai Caldera, Isabel Cristina Lemos Baltazar, Raquel Regina Bonelli, Guilherme Santoro-Lopes, Lee Woodland Riley, Beatriz Meurer Moreira
INFECTION GENETICS AND EVOLUTION
(2020)
Article
Microbiology
Eloiza H. Campana, Stephanie G. Chuster, Isadora R. da Silva, Raphael P. Paschoal, Raquel R. Bonelli, Beatriz M. Moreira, Renata C. Picao
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2017)
Article
Infectious Diseases
Ana Paula de Souza da-Silva, Viviane Santos de Sousa, Natacha Martins, Rubens Clayton da Silva Dias, Raquel Regina Bonelli, Lee W. Riley, Beatriz Meurer Moreira
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2017)
Article
Microbiology
Ana F. M. Martins, Herrison Fontana, Beatriz M. Moreira, Raquel R. Bonelli
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2018)