Journal
DRUG METABOLISM AND PHARMACOKINETICS
Volume 23, Issue 3, Pages 175-180Publisher
JAPANESE SOC STUDY XENOBIOTICS
DOI: 10.2133/dmpk.23.175
Keywords
metabolites; glycyrrhetic acid; bile; diconjugate; LC-MS
Categories
Funding
- National Natural Science Foundation of China [30472060]
- China 863 Plan [2003AA2Z347A]
Ask authors/readers for more resources
To study the metabolites of glycyrrhetic acid (GA) in rat bile, an analytical method was developed to identify GA and its metabolites by liquid chromatography mass spectrometry (LC-MS). Rat bile was collected after i.v. injection of GA. Three major GA-related peaks were detectable in rat bile by high-performance liquid chromatography (HPLC) analysis at 254 nm. LC-MS spectra showed their protonated molecular ions at m/z 727, 647, and 551. Furthermore, the three metabolites were also confirmed to exist in rat bile on LC-MS total ion chromatogram (TIC). Taken together with the susceptible nature to beta-glucuronidase digestion and alkaline conditions, they were identified to be a novel sulfate-glucuronide diconjugate and the known monoglucuronide and sulfate conjugate, respectively.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available