Article
Pharmacology & Pharmacy
Melina Lopez, Pedro F. Malacarne, Anna Gajos-Draus, Xinxin Ding, Andreas Daiber, Jon O. Lundberg, Stefan Offermanns, Ralf P. Brandes, Flavia Rezende
Summary: The study suggests that metabolism by vascular CYPs does not contribute to the pharmacological function of organic nitrates, while ALDH2 plays an important role in activating organic nitrates.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marta Nowak, Katarzyna Zawadzka, Janusz Szemraj, Aleksandra Goralczyk-Binkowska, Katarzyna Lisowska
Summary: This study investigated the biodegradation mechanism of chloroxylenol by fungal strains and analyzed the ecotoxicity of emerging by-products. The results showed a high elimination rate of PCMX by the tested microorganisms, and the identification of new fungal by-products. Furthermore, the study revealed a detoxifying nature of the biodegradation process of PCMX.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Seth A. Strom, Aaron G. Hager, Jeanaflor Crystal T. Concepcion, Nicholas J. Seiter, Adam S. Davis, James A. Morris, Shiv S. Kaundun, Dean E. Riechers
Summary: The resistance of waterhemp to the herbicide S-metolachlor involves a combination of Phase I and Phase II metabolic activities, specifically the O-demethylation reaction conferring resistance. Greater activities of GSTs and P450s were observed in resistant populations compared to sensitive populations, leading to increased metabolism of S-metolachlor and the formation of resistant metabolites.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nadezhda T. Doncheva, Oana Palasca, Reza Yarani, Thomas Litman, Christian Anthon, Martien A. M. Groenen, Peter F. Stadler, Flemming Pociot, Lars J. Jensen, Jan Gorodkin
Summary: Animal models are vital for studying molecular pathways in human diseases, but the conservation of tissue expression between species is still unclear. By comparing tissue expression data in animal models, we can identify pathways that are more consistent with human pathways. While mouse data is more comprehensive, differences among species should be considered when studying pathway activity.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Genetics & Heredity
Shuai Zhang, Song Gao, Yu Chen, Sha Xu, Shiqin Yu, Jingwen Zhou
Summary: The leaves of Ampelopsis grossedentata are rich in dihydromyricetin, which has many potential applications. In this study, hydroxylase genes involved in the biosynthesis of dihydromyricetin were identified and characterized. The hydroxylation process and carbon flux pathways of dihydromyricetin in Ampelopsis grossedentata were successfully identified.
Article
Engineering, Environmental
Yuhao Chu, Chaofan Zhang, Rupeng Wang, Xi Chen, Nanqi Ren, Shih-Hsin Ho
Summary: This study investigated the biotransformation of sulfamethoxazole by microalgae, particularly highlighting the role of CYP450 enzymes in the degradation process. The research elucidated the SMX biodegradation pathway involving CYP450 in microalgae, providing insights for using microalgae-mediated CYP450 to eliminate antibiotics.
Article
Chemistry, Physical
Ya Wang, Ana Luisa Malaco Morotti, Yiren Xiao, Zhuo Wang, Song Wu, Jianghua Chen, Evangelos C. Tatsis
Summary: This study characterized three cytochrome P450 enzymes from the Garcinia xanthochymus tree and found that they play important roles in the biosynthesis of benzophenones and xanthones. Mutagenesis experiments on CYP3 revealed the key role of the V375 residue in product diversity. Engineering of the mutant enzyme further improved product specificity and catalytic transformation.
Article
Chemistry, Physical
Ya Wang, Ana Luisa Malaco Morotti, Yiren Xiao, Zhuo Wang, Song Wu, Jianghua Chen, Evangelos C. Tatsis
Summary: In this study, three CYP enzymes from the Garcinia xanthochymus tree were characterized for their roles in the biosynthesis pathways of benzophenones and xanthones. Through engineering experiments, product specificity and catalytic transformation efficiency were improved.
Article
Engineering, Environmental
Yuhao Chu, Chaofan Zhang, Rupeng Wang, Xi Chen, Nanqi Ren, Shih-Hsin Ho
Summary: This study elucidated the SMX biodegradation pathway involving CYP450 in microalgae and revealed key details of the hydroxylamine group. It advances the development of using microalgae-mediated CYP450 to eliminate antibiotics.
Article
Biochemistry & Molecular Biology
He Liu, Zixia Hu, Ningning Han, Yi Yang, Guode Zhao, Mengdie Su, Yue Zhang, Weiwei Li, Ying Peng, Jiang Zheng
Summary: The study aimed to investigate the metabolic activation of MTX and its possible correlation with cytotoxicity. An oxidative metabolite (M1) and a GSH conjugate (M2) were observed in S9 fraction incubations and rat primary hepatocyte culture after MTX exposure. M1 and M2 were also detected in the bile of MTX-treated rats. CYP2A6 was found to be the dominant enzyme responsible for the oxidation of MTX. Inhibition of CYP2A6 and sulfotransferase significantly decreased the formation of M2. Pretreatment of primary hepatocytes with inhibitors reduced the susceptibility to MTX-induced cytotoxicity.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Medicine, Research & Experimental
Y. Gloor, C. Lloret-Linares, M. Bosilkovska, N. Perroud, H. Richard-Lepouriel, J. -m. Aubry, Y. Daali, J. A. Desmeules, M. Besson
Summary: Cytochromes from the P450 family have a central role in the metabolism of antidepressants, but the variability in drug metabolic capacities among individuals can present risks and complicate drug prescription. This study investigates the genetic and environmental factors contributing to decreased cytochrome activities in a cohort of antidepressant-treated patients. The results reveal a significant rate of phenoconversion and reduced activities of certain cytochromes, highlighting the importance of phenotyping and the risks in a polymedicated population.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Plant Sciences
Jianwen Xu, Jun Zhao, Jianguang Liu, Chengguang Dong, Liang Zhao, Nijiang Ai, Peng Xu, Guoli Feng, Zhenzhen Xu, Qi Guo, Junling Cheng, Yueping Wang, Xin Wang, Ningshan Wang, Songhua Xiao
Summary: In this study, the important role of GbCYP72A1 genes in plant response and resistance to Verticillium wilt (VW) was revealed. Through quantitative trait loci (QTL) mapping, a cytochrome P450 (CYP) gene associated with resistance to the nondefoliated strain of the fungal pathogen was identified. Transcriptome sequencing and pathway enrichment analyses showed that the GbCYP72A1 genes primarily affected disease resistance through plant hormone signal transduction, plant-pathogen interaction, and mitogen-activated protein kinase (MAPK) signaling pathways. Furthermore, the difference in disease resistance ability between GbCYP72A1d and GbCYP72A1a was potentially attributed to a synaptic structure present in the GbCYP72A1d protein.
Review
Biotechnology & Applied Microbiology
Yifeng Zhang, Lin Ma, Ping Su, Luqi Huang, Wei Gao
Summary: Terpenoids, as the largest family of natural products, play significant roles in medicine, agriculture, cosmetics, and food. Traditional extraction methods have low yields, waste resources, and are not suitable for endangered species. Modern biotech approaches are promising in discovering and characterizing plant terpenoid-related P450 enzymes, and metabolic engineering strategies can be employed to increase terpenoid production.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Toxicology
Slobodan P. Rendic, F. Peter Guengerich
Summary: This overview discusses the metabolic activation of various compounds by cytochrome P450 enzymes, with a focus on bioactivation reactions. The study identifies a higher level of activation for general chemicals, drugs, and natural products compared to physiological compounds. Enzymes such as P450 3A4, 1A2, 2E1, and 1A1 are highlighted for their roles in catalyzing the formation of activated metabolites from different compound groups.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Marcelo R. A. De Figueiredo, Hamlin Barnes, Claudia M. Boot, Ana Beatriz T. B. De Figueiredo, Scott J. Nissen, Franck E. Dayan, Todd A. Gaines
Summary: Amaranthus tuberculatus, a waterhemp plant species resistant to 2,4-dichlorophenoxyacetic acid (2,4-D), has been found to possess rapid metabolic detoxification ability. Through a novel detoxification mechanism, resistant plants hydroxylate 2,4-D into a less toxic metabolite, while susceptible plants conjugate 2,4-D to aspartate. This evolution of detoxification reactions in resistant plants allows for more efficient detoxification of 2,4-D.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemical Research Methods
Yunping Huang, Jinmei Fu, Richard Ludwig, Li Tao, Jacob Bongers, Li Ma, Ming Yao, Mingshe Zhu, Tapan Das, Reb Russell
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2017)
Article
Biochemical Research Methods
Kai Cao, John A. Brailsford, Ming Yao, Janet Caceres-Cortes, Robert Espina, Samuel J. Bonacorsi
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
(2017)
Article
Pharmacology & Pharmacy
Ming Yao, Bingming Chen, Weiping Zhao, John T. Mehl, Lingjun Li, Mingshe Zhu
DRUG METABOLISM AND DISPOSITION
(2018)
Review
Biochemical Research Methods
Ming Yao, Nuggehally R. Srinivas
BIOMEDICAL CHROMATOGRAPHY
(2009)
Article
Biochemical Research Methods
Ming Yao, Nuggehally R. Srinivas
BIOMEDICAL CHROMATOGRAPHY
(2012)
Article
Pharmacology & Pharmacy
Sreeneeranj Kasichayanula, Xiaoni Liu, Melanie Pe Benito, Ming Yao, Marc Pfister, Frank P. LaCreta, William Griffith Humphreys, David W. Boulton
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2013)
Article
Chemistry, Medicinal
Wenying Jian, Ming Yao, Duxi Zhang, Mingshe Zhu
CHEMICAL RESEARCH IN TOXICOLOGY
(2009)
Article
Pharmacology & Pharmacy
M. Obermeier, M. Yao, A. Khanna, B. Koplowitz, M. Zhu, W. Li, B. Komoroski, S. Kasichayanula, L. Discenza, W. Washburn, W. Meng, B. A. Ellsworth, J. M. Whaley, W. G. Humphreys
DRUG METABOLISM AND DISPOSITION
(2010)
Article
Pharmacology & Pharmacy
Lifei Wang, Donglu Zhang, Nirmala Raghavan, Ming Yao, Li Ma, Charles A. Frost, Brad D. Maxwell, Shiang-yuan Chen, Kan He, Theunis C. Goosen, W. Griffith Humphreys, Scott J. Grossman
DRUG METABOLISM AND DISPOSITION
(2010)
Article
Biochemical Research Methods
Ming Yao, Li Ma, Eva Duchoslav, Mingshe Zhu
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2009)
Article
Chemistry, Analytical
Naiyu Zheng, Ian M. Catlett, Kristin Taylor, Huidong Gu, Mark A. Pattoli, Robert J. Neely, Wenying Li, Alban Allentoff, Xiling Yuan, Eugene Ciccimaro, Ming Yao, Bethanne M. Warrack, James R. Burke, Yan J. Zhang, Renuka Pillutla, Jianing Zeng
ANALYTICAL CHEMISTRY
(2019)
Article
Medicine, Research & Experimental
Hong Shen, Ming Yao, Michael Sinz, Punit Marathe, A. David Rodrigues, Mingshe Zhu
MOLECULAR PHARMACEUTICS
(2019)
Article
Pharmacology & Pharmacy
Ming Yao, Tingting Cai, Eva Duchoslav, Li Ma, Xu Guo, Mingshe Zhu
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2020)
Article
Pharmacology & Pharmacy
Hong Shen, Hong Su, Tongtong Liu, Ming Yao, Gabe Mintier, Lun Li, R. Marcus Fancher, Ramaswamy Iyer, Punit Marathe, Yurong Lai, A. David Rodrigues
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2015)