Journal
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
Volume 34, Issue 12, Pages 1297-1305Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/03639040802071661
Keywords
aquasomes; nanoparticles; hydroxyapatite; vaccines; bovine serum albumin; trehalose; cellobiose
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Funding
- Department of Biotechnology, New Delhi
- Council for Scientific and Industrial Research, New Delhi
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The development of compound that enhances immune responses to recombinant or synthetic epitopes is of considerable importance in vaccine research. Of the many different types of immunopotentiating compounds that have been researched, aquasomes are of considerable promise, because of their potency and adjuvanticity. Aquasomes were prepared by self-assembling of hydroxyapatite by co-precipitation method and thereafter preliminary coated with polyhydroxyl oligomers (cellobiose and trehalose) and subsequently adsorbed with bovine serum albumin (BSA) as a model antigen. The prepared systems were characterized for size, shape, antigen-loading efficiency, in vitro antigen stability, and in vivo performance. BSA-immobilized aquasomes were around 200 nm in diameter and spherical in shape and had approximately 20-30% BSA-loading efficiency. The immunological activity of the formulated aquasomes was compared with plain BSA and better results were observed. Studies also indicated that aquasome formulations could elicit combined T-helper 1 (Th1) and Th2 immune response.
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