Comparing the diel vertical migration of Karlodinium veneficum (dinophyceae) and Chattonella subsalsa (Raphidophyceae): PSII photochemistry, circadian control, and carbon assimilation

Diel vertical migration (DVM) is thought to provide an adaptive advantage to some phytoplankton, and may help determine the ecological niche of certain harmful algae. Here we separately compared DVM patterns between two species of harmful algae isolated from the Delaware Inland Bays, Karlodinium veneficum and Chattonella subsalsa, in laboratory columns. We interpreted the DVM patterns of each species with Photosystem II (PSII) photochemistry, rates of carbon assimilation, and specific growth rates. Each species migrated differently, wherein K. veneficum migrated closer to the surface each day with high population synchrony, while C. subsalsa migrated near to the surface from the first day of measurements with low population synchrony. Both species appeared to downregulate PSII in high light at the surface, but by different mechanisms. C. subsalsa grew slower than K. veneficum in low light intensities (approximate to bottom of columns), and exhibited maximal rates of C-assimilation (Pr-max) at surface light intensities, suggesting this species may prefer high light, potentially explaining this species' rapid surface migration. Contrastingly, K. veneficum showed declines in carbon assimilation at surface light intensities, and exhibited a smaller reduction in growth at low (bottom) light intensities (compared to C. subsalsa), suggesting that this species' step-wise migration was photoacclimative and determined daily migration depth. DVM was found to be under circadian control in C. subsalsa, but not in K. veneficum. However, there was little evidence for circadian regulation of PSII photochemistry in either species. Migration conformed to each species' physiology, and the results contribute to our understanding each alga's realized environmental niche. (C) 2014 Elsevier B.V. All rights reserved.