Article
Agriculture, Dairy & Animal Science
Cecily Bishop, Vimal Selvaraj, David H. Townson, Joy L. Pate, Milo C. Wiltbank
Summary: The corpus luteum (CL) forms from the cellular remnants of the follicle and produces progesterone required for successful pregnancy. The CL and the embryo have a symbiotic relationship, both relying on each other for survival. The Northeast Multistate Project has contributed to our understanding of the regulation and function of the CL in cattle, providing valuable insights for improving fertility.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Thiago O. Cunha, Leah R. Statz, Rafael R. Domingues, Joao Paulo N. Andrade, Milo C. Wiltbank, Joao Paulo N. Martins
Summary: The objective of this study was to investigate the effects of inducing accessory corpus luteum on follicular and luteal dynamics in lactating Holstein cows. The results showed that inducing accessory corpus luteum not only altered the dynamics of follicles and corpus luteum but also deferred and prolonged the process of luteolysis.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Jaya Bharati, N. H. Mohan, Satish Kumar, Jayashree Gogoi, Sai Kumar, Bosco Jose, Meeti Punetha, Sanjib Borah, Amit Kumar, Mihir Sarkar
Summary: RNA-Seq was used to identify differentially expressed genes (DEGs) in porcine corpus luteum (CL) during different physiological stages, revealing significant enrichment of gene functions in early, mid, and late stages. Apoptosis was most active in the regressed stage. Pathway analysis indicated lipid metabolism, translation, immune, and endocrine system pathways were associated with CL function. Gene network analysis identified novel hub genes AR, FOS, CDKN1A potentially regulating CL physiology.
Article
Reproductive Biology
Megan A. Mezera, Wenli Li, Lihe Liu, Rina Meidan, Francisco Penagaricano, Milo C. Wiltbank
Summary: Research suggests that early subluteolytic PGF pulses impact the luteal transcriptome during natural luteolysis, though not by directly affecting steroidogenesis or inhibiting cellular function. Instead, these early pulses mainly alter pathways related to local steroid and glutathione metabolism.
BIOLOGY OF REPRODUCTION
(2021)
Article
Cell Biology
Ewa Mlyczynska, Marta Kiezun, Patrycja Kurowska, Monika Dawid, Karolina Pich, Natalia Respekta, Mathilde Daudon, Edyta Rytelewska, Kamil Dobrzyn, Barbara Kaminska, Tadeusz Kaminski, Nina Smolinska, Joelle Dupont, Agnieszka Rak
Summary: In this review, we summarize the current knowledge about the expression and role of adipokines and neuropeptides in the physiological regulation of corpus luteum function, as well as their potential involvement in pathologies affecting the luteal cells that disrupt the estrous cycle.
Article
Agriculture, Dairy & Animal Science
Barbara Maria Socha, Piotr Lada, Agnieszka Walentyna Jonczyk, Anna Justyna Korzekwa, Dariusz Jan Skarzynski
Summary: The aim of this study was to determine the expression of PPAR isoforms in the bovine corpus luteum throughout the estrous cycle, and their involvement in PGF(2 alpha)-induced processes related to luteolysis. The results showed that the mRNA and protein expression of PPARs changes in the bovine corpus luteum throughout the estrous cycle and under the influence of PGF(2 alpha). PPAR gamma, among all examined isoforms, could be involved in the regulation of PGF(2 alpha)-induced luteolysis in cattle.
Article
Biology
Meeti Punetha, Sai Kumar, Avishek Paul, Bosco Jose, Jaya Bharati, Arvind Sonwane, Jonathan A. Green, Kristin Whitworth, Mihir Sarkar
Summary: This study aimed to better understand the role of EGR1 in transactivation of TGF beta 1 during PGF2 alpha induced luteal regression using buffalo luteal cells. The results showed that EGR1 and TGF beta 1 mRNA expression were significantly upregulated at 12 hours post PGF2 alpha induction, and knocking out EGR1 did not modulate the expression of TGF beta 1. Additionally, the Caspase 3 mRNA expression was significantly increased in EGR1 KO luteal cells treated with PGF2 alpha compared to wild type luteal cells.
BIOLOGICAL RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Sang-Hee Lee, Seunghyung Lee
Summary: The study found that PGF2 alpha affects cell adhesion in bovine luteal theca cells by disrupting cytokeratin, vimentin, and desmoplakin proteins, as well as increasing ROCK protein levels, leading to decreased cell-cell adhesion during corpus luteum regression.
Article
Reproductive Biology
Mariana Diel de Amorim, Sarah A. Bramer, Gayathri D. Rajamanickam, Claudia Klein, Claire Card
Summary: The study investigated the changes in endometrial and luteal gene expression during different reproductive stages and treatments in horses. The results showed that serum LNPEP concentration was higher in pregnant and oxytocin-treated mares compared to diestrous mares. Endometrial PTGES expression was higher in diestrous, pregnant, and oxytocin-treated groups. Carbetocin treatment decreased serum progesterone and LNPEP, increased endometrial PLA2G2C expression, and decreased endometrial PTGES and luteal aromatase. Meclofenamic acid treatment decreased endometrial PLA2G2C, increased endometrial PTGES, and resulted in less OXTR and OXT luteal abundance. The expression of LNPEP is involved in luteal function and pregnancy recognition pathways in horses.
Review
Physiology
Corrine F. Monaco, John S. Davis
Summary: The corpus luteum is a transient endocrine gland in the ovary that produces progesterone for pregnancy. The formation and function of this gland rely on angiogenesis, which establishes the necessary blood flow and microvasculature. During regression, the corpus luteum undergoes structural changes and the blood vessels regress, impacting progesterone production. Factors released during luteolysis play a role in these processes, but the exact mechanisms remain unknown.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Veterinary Sciences
Fang Chen, Yi'an Hou, Xiaoqing Zhu, Cheng Mei, Rihong Guo, Zhendan Shi
Summary: In recent decades, embryo transfer has been widely used in dairy cows, but improving the conception rate after transfer remains a major challenge. This review conducted a meta-analysis to evaluate the efficacy of hCG and GnRH in improving pregnancy rates after transfer. The results showed that hCG and GnRH can improve pregnancy rates in cows with poor fertility, but not in cows with good fertility.
VETERINARY SCIENCES
(2023)
Article
Reproductive Biology
Rafael R. Domingues, O. J. Ginther, Victor Gomez-Leon, Thadeu Castro, Milo C. Wiltbank
Summary: This study demonstrates that a single minor PGF treatment can upregulate endogenous PGF synthesis in heifers and mares through distinct regulatory mechanisms, stimulating endogenous PGF synthesis. This results in a transient decrease in P4 in heifers but not in mares, and leads to changes in gene expression in both species.
BIOLOGY OF REPRODUCTION
(2022)
Article
Agriculture, Dairy & Animal Science
Granit Thaqi, Bajram Berisha, Michael W. Pfaffl
Summary: The study aimed to evaluate the mRNA expression levels of various local novel adipokines, including vaspin, adiponectin, visfatin, and resistin, along with their associated receptors, in the corpus luteum (CL) during luteal regression in dairy cows. The results showed significant fluctuations in the expression of adipokines, suggesting their potential roles in regulating the process of luteal regression and subsequent structural changes in the ovary.
Article
Reproductive Biology
Corrine F. Monaco, Michele R. Plewes, Emilia Przygrodzka, Jitu W. George, Fang Qiu, Peng Xiao, Jennifer R. Wood, Andrea S. Cupp, John S. Davis
Summary: During the regression of the ovarian corpus luteum, fibroblasts are responsible for rapid matrix remodeling. However, little is known about the role of fibroblasts in the functional or regressing corpus luteum. In this study, it was found that FGF2 activates luteal fibroblasts, leading to collagen production and cell proliferation.
BIOLOGY OF REPRODUCTION
(2023)
Article
Veterinary Sciences
Uxia Yanez, Antonio V. Murillo, Juan J. Becerra, Pedro G. Herradon, Ana I. Pena, Luis A. Quintela
Summary: The aim of this study was to compare the accuracy of different methods in assessing corpus luteum functionality. Results showed that the largest diameter measurement was more accurate than subjective size in Experiment 1. In Experiment 2, corpus luteum area was found to be the best indicator, although subjective and objective corpus luteum blood flow also provided accurate information 24 hours after injection.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Jakob Albrecht, Samantha Exner, Carsten Groetzinger, Sonal Prasad, Frank Konietschke, Nicola Beindorff, Anja A. Kuhl, Vikas Prasad, Winfried Brenner, Eva J. Koziolek
Summary: Peptide receptor radionuclide therapy (PRRT) using radiolabeled somatostatin receptor (SSTR) analogs is a common approach in advanced neuroendocrine neoplasms. Recently, SSTR antagonists have shown promising results for imaging and therapy due to a higher number of binding sites than in commonly used agonists. Lu-177-DOTA-JR11 exhibited a higher tumor-to-kidney ratio, increased G2/M phase accumulation of cells, decreased tumor size, prolonged median survival, and stable tumor uptake over 2 treatment cycles compared to Lu-177-DOTATOC.
JOURNAL OF NUCLEAR MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
David Bode, Lukas Semmler, Paulina Wakula, Niklas Hegemann, Uwe Primessnig, Nicola Beindorff, David Powell, Raphael Dahmen, Hartmut Ruetten, Christian Oeing, Alessio Alogna, Daniel Messroghli, Burkert M. Pieske, Frank R. Heinzel, Felix Hohendanner
Summary: This study found that the SGLT-1&2 inhibitor sotagliflozin improved left atrial remodeling in a metabolic HFpEF rat model, reduced the occurrence of cellular arrhythmias, and enhanced mitochondrial function. These effects may be related to the inhibition of sodium-calcium exchanger (NCX) forward-mode activity.
CARDIOVASCULAR DIABETOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Isabelle Heing-Becker, Carsten Grotzinger, Nicola Beindorff, Sonal Prasad, Sarah Erdmann, Samantha Exner, Rainer Haag, Kai Licha
Summary: Modular synthesis methods were utilized to synthesize a new asymmetric bifunctional cyanine dye as a structural and functional linker for constructing hybrid imaging probes, which was introduced into Ga-68-DOTATATE radiopeptide targeting SSTR2-overexpressing neuroendocrine tumors, allowing targeted imaging with high affinity and uptake.
Article
Oncology
Jan Lisec, Dennis Kobelt, Wolfgang Walther, Margarita Mokrizkij, Carsten Groetzinger, Carsten Jaeger, Katharina Baum, Mareike Simon, Jana Wolf, Nicola Beindorff, Winfried Brenner, Ulrike Stein
Summary: MACC1 acts as an important regulator of cancer metabolism by altering the utilization of glucose, glutamine, and pyruvate, thereby supporting metabolic activities in cancer cells.
Article
Cardiac & Cardiovascular Systems
Niklas Hegemann, Uwe Primessnig, David Bode, Paulina Wakula, Nicola Beindorff, Robert Klopfleisch, Laura Michalick, Jana Grune, Felix Hohendanner, Daniel Messroghli, Burkert Pieske, Wolfgang M. Kuebler, Frank R. Heinzel
Summary: Right ventricular dysfunction in obese ZSF-1 rats with HFpEF is associated with intrinsic RV cardiomyocyte remodelling including reduced cytosolic Ca2+ amplitudes, loss of frequency-dependent augmentation of Ca2+ release, and increased myofilament Ca2+ sensitivity.
Article
Biochemistry & Molecular Biology
Akvile Haeckel, Lena Ascher, Nicola Beindorff, Sonal Prasad, Karolina Garczynska, Jing Guo, Eyk Schellenberger
Summary: XTEN864-anxA5, a fusion protein with an 864-amino-acid XTEN polypeptide and Annexin A5, has a long circulating half-life in mice after intravenous injection, indicating its potential as a therapeutic drug with promising applications in cardiovascular diseases and cancer. Additionally, XTEN864-anxA5 was found to efficiently target specific tissue regions, such as atherosclerotic plaques and the adventitia, demonstrating its potential for targeted therapy.
Article
Computer Science, Interdisciplinary Applications
Mathias Lukas, Anne Kluge, Nicola Beindorff, Winfried Brenner
Summary: Recent advances in clinical SPECT instrumentation enable improvements in non-standard multi-isotope experiments at the edge of physical feasibility, enhancing the efficiency of pharmaceutical research. The development of a Monte Carlo simulation model allows for high-accuracy energy spectra, planar images, and tomographic reconstructions for multi-isotope experiments.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2021)
Article
Multidisciplinary Sciences
Nicola Beindorff, Fabian Schmitz-Peiffer, Daniel Messroghli, Winfried Brenner, Janet F. Eary
Summary: Functional imaging of European round back slug (Arionidae) and leopard slug (Limacidae) was conducted using radionuclide imaging with SPECT and PET, combined with MRI and CT for anatomic definition. The study established an imaging protocol for comparative species study and described the detailed functional anatomy of the slugs, including organ biodistribution patterns.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Giulia Sivelli, Gaurasundar M. Conley, Carolina Herrera, Kathryn Marable, Kyle J. Rodriguez, Heinrich Bollwein, Mateus J. Sudano, Jurgen Brugger, Andre J. Simpson, Giovanni Boero, Marco Grisi
Summary: The article discusses the potential of using NMR for analyzing microscopic entities, such as early-stage mammalian embryos. It introduces a device that combines ultra-compact micro-chip technologies and 3D-printed micro-structures to overcome handling and sensitivity issues. The study demonstrates the device's ability to obtain NMR spectra from bovine pre-implantation embryos, indicating its potential for non-invasive embryo selection prior to transfer.
JOURNAL OF MAGNETIC RESONANCE
(2022)
Article
Biochemistry & Molecular Biology
Preeti Bakrania, Gareth Hall, Yvonne Bouter, Caroline Bouter, Nicola Beindorff, Richard Cowan, Sarah Davies, Jemma Price, Chido Mpamhanga, Elizabeth Love, David Matthews, Mark D. Carr, Thomas A. Bayer
Summary: New routes for active and passive immunisation against Alzheimer's disease (AD) have been discovered through the identification of a unique conformational epitope in the N-terminal region of the A beta protein. A vaccine called TAPAS showed promising results in reducing amyloid-plaque formation, rescuing brain glucose metabolism, stabilizing neuron loss, and improving memory deficiencies in mouse models of AD. Treatment with a humanised version of the TAP01 antibody also had positive effects in both models.
MOLECULAR PSYCHIATRY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Dirk Zboralski, Aileen Hoehne, Anne Bredenbeck, Anne Schumann, Minh Nguyen, Eberhard Schneider, Jan Ungewiss, Matthias Paschke, Christian Haase, Jan L. von Hacht, Tanya Kwan, Kevin K. Lin, Jan Lenore, Thomas C. Harding, Jim Xiao, Andrew D. Simmons, Ajay-Mohan Mohan, Nicola Beindorff, Ulrich Reineke, Christiane Smerling, Frank Osterkamp
Summary: The study confirms the high expression of FAP-2286 in multiple tumor types and demonstrates its ability to have persistent uptake and retention in FAP-positive tumors, suggesting its potential for imaging and therapeutic applications.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Article
Multidisciplinary Sciences
Nadine Curdt, Franziska W. Schmitt, Caroline Bouter, Trendelina Iseni, Hanna C. Weile, Berfin Altunok, Nicola Beindorff, Thomas A. Bayer, Matthew B. Cooke, Yvonne Bouter
Summary: Spatial disorientation and allocentric deficits can be detected in early stages of Alzheimer's disease, and analyzing search strategies in rodent models can be a powerful tool for preclinical drug testing and early therapeutic successes.
SCIENTIFIC REPORTS
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Nicola Beindorff, Nikolaos Papadopoulos, Stefan Hoffmann, Ajay-Mohan Mohan, Mathias Lukas, Winfried Brenner, Paul-Georg Jost-Brinkmann, Thomas Prager
Summary: The study aimed to investigate the impact of piezocision on bone remodeling in rats using bone SPECT imaging. The results showed measurable tooth movement induced by orthodontic appliances, but the additional effect of piezocision on bone remodeling was not significant.
NUKLEARMEDIZIN-NUCLEAR MEDICINE
(2022)
Article
Clinical Neurology
Caroline Bouter, Frederik Wilhelm Ott, Daniel Guenther, Lukas Weig, Fabian Schmitz-Peiffer, Mahriban Rozyyeva, Nicola Beindorff, Yvonne Bouter
Summary: There is increasing evidence that cannabinoids have promising medicinal and pharmacological effects, but the growing interest in medical cannabis highlights the need to understand the brain alterations associated with cannabinoids and clinical and behavioral phenotypes. This study investigated the effects of long-term WIN 55,212-2 treatment on cerebral metabolism and memory function in mice. The findings suggest that WIN 55,212-2 treatment leads to brain metabolism changes and memory deficits, particularly when administered during early adulthood.
JOURNAL OF NEURAL TRANSMISSION
(2023)
Article
Dentistry, Oral Surgery & Medicine
Nikolaos Papadopoulos, Nicola Beindorff, Stefan Hoffmann, Paul-Georg Jost-Brinkmann, Thomas Michael Praeger
Summary: This study found that piezocision can accelerate tooth movement while also causing increased root resorption in an experiment involving 10 rats. There was a strong correlation between the speed of tooth movement and root resorption on the side with piezocision, whereas the control side showed only a weak correlation.
KOREAN JOURNAL OF ORTHODONTICS
(2021)
Article
Agriculture, Dairy & Animal Science
Mehak Nisar, Shafayat Ahmad Beigh, Abdul Qayoom Mir, Syed Ashaq Hussain, Aijaz Ahmad Dar, Iqbal Yatoo, Adil Mehraj Khan
Summary: This study aimed to evaluate the role of vitamin D in redox balance, insulin resistance, and its predicting value for subclinical pregnancy toxemia (SPT) in pregnant ewes. The results showed that ewes with insufficient vitamin D had significantly lower total antioxidant capacity (TAC) compared to ewes with sufficient vitamin D, and there were significant differences in metabolite concentrations between the two groups. Additionally, vitamin D was positively correlated with fructosamine, calcium, and TAC, and negatively correlated with non-esterified fatty acid (NEFA) and total oxidant status (TOS). Ewes with insufficient vitamin D had a higher risk of developing SPT two weeks prior to lambing and at lambing.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2024)
Article
Agriculture, Dairy & Animal Science
L. G. Gonzalez-Arostegui, A. Munoz-Prieto, C. P. Rubio, J. J. Ceron, L. Bernal, I. Rubic, V. Mrljak, J. C. Gonzalez-Sanchezc, A. Tvarijonaviciute
Summary: This study investigated changes in the saliva and serum proteome of dogs with hypothyroidism using TMT labelling and LC-MS/MS. The findings showed that this disease induces alterations in the saliva and serum proteome, reflecting physiological and pathological changes in the immune system, metabolism, skin, and coagulation. The downregulation of LDH in saliva may serve as a potential biomarker for canine hypothyroidism.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2024)
Article
Agriculture, Dairy & Animal Science
Camila R. C. Brito, Agatha D. Cordeiro, Pricila Baldessar, Carolini Schultz, Monike Quirino, Rafael R. Ulguim, Paulo B. D. Goncalves, Thomaz Lucia Jr, Ivan Bianchi, Bernardo G. Gasperin
Summary: This study tested synchronization protocols without progestogens during the luteal phase in gilts. The results showed that the protocols based on PGF and hCG were effective in reducing the interval between estrus in gilts. Additionally, the hCG2 protocol had negative effects on the number of piglets born alive and total litter size.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2024)
Article
Agriculture, Dairy & Animal Science
F. Frers, J. Delarocque, K. Feige, K. Huber, T. Warnken
Summary: This study aimed to investigate the relationship between insulin dysregulation and the expression and phosphorylation of key proteins in the insulin signaling cascade in different tissues of horses. The results showed enhanced phosphorylation of the insulin receptor and protein kinase B in response to stimulation, while phosphorylation of adenosine monophosphate protein kinase alpha decreased after glucose administration. Interestingly, horses with insulin dysregulation had significantly higher phosphorylation of mechanistic target of rapamycin in muscle tissue. These findings suggest that insulin signaling is maintained in horses, but post-receptor alterations may occur.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2024)
Article
Agriculture, Dairy & Animal Science
Patrice Dufour, Vincent Frisee, Goulven Rigaux, Flore Brutinel, Sophie Egyptien, Philippe Bossaert, Jessica Deleersnyder, Stefan Deleuze, Stephanie Peeters, Caroline Le Goff, Jerome Ponthier, Etienne Cavalier
Summary: This report describes the method of measuring progesterone and estrone-sulfate using Liquid Chromatography coupled with Mass Spectrometry in American Bison feces. This method provides an alternative to serum assay for studying the pregnancy-related evolution of these hormones in American Bison.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2024)