Article
Veterinary Sciences
Agnieszka W. Jonczyk, Katarzyna K. Piotrowska-Tomala, Dariusz J. Skarzynski
Summary: This study investigated the effects of direct injection of different doses of dinoprost into the bovine corpus luteum on hormonal concentrations and mRNA levels. It also examined the effects of dinoprost on luteal function and vascular network status. The results showed that injection of dinoprost directly into the corpus luteum can affect luteal function by modulating these indicators.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Reproductive Biology
Megan A. Mezera, Wenli Li, Lihe Liu, Rina Meidan, Francisco Penagaricano, Milo C. Wiltbank
Summary: Research suggests that early subluteolytic PGF pulses impact the luteal transcriptome during natural luteolysis, though not by directly affecting steroidogenesis or inhibiting cellular function. Instead, these early pulses mainly alter pathways related to local steroid and glutathione metabolism.
BIOLOGY OF REPRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Sang-Hee Lee, Seunghyung Lee
Summary: The study found that PGF2 alpha affects cell adhesion in bovine luteal theca cells by disrupting cytokeratin, vimentin, and desmoplakin proteins, as well as increasing ROCK protein levels, leading to decreased cell-cell adhesion during corpus luteum regression.
Article
Agriculture, Dairy & Animal Science
Barbara Maria Socha, Piotr Lada, Agnieszka Walentyna Jonczyk, Anna Justyna Korzekwa, Dariusz Jan Skarzynski
Summary: The aim of this study was to determine the expression of PPAR isoforms in the bovine corpus luteum throughout the estrous cycle, and their involvement in PGF(2 alpha)-induced processes related to luteolysis. The results showed that the mRNA and protein expression of PPARs changes in the bovine corpus luteum throughout the estrous cycle and under the influence of PGF(2 alpha). PPAR gamma, among all examined isoforms, could be involved in the regulation of PGF(2 alpha)-induced luteolysis in cattle.
Article
Reproductive Biology
Rafael R. Domingues, O. J. Ginther, Victor Gomez-Leon, Thadeu Castro, Milo C. Wiltbank
Summary: This study demonstrates that a single minor PGF treatment can upregulate endogenous PGF synthesis in heifers and mares through distinct regulatory mechanisms, stimulating endogenous PGF synthesis. This results in a transient decrease in P4 in heifers but not in mares, and leads to changes in gene expression in both species.
BIOLOGY OF REPRODUCTION
(2022)
Article
Agriculture, Dairy & Animal Science
Cecily Bishop, Vimal Selvaraj, David H. Townson, Joy L. Pate, Milo C. Wiltbank
Summary: The corpus luteum (CL) forms from the cellular remnants of the follicle and produces progesterone required for successful pregnancy. The CL and the embryo have a symbiotic relationship, both relying on each other for survival. The Northeast Multistate Project has contributed to our understanding of the regulation and function of the CL in cattle, providing valuable insights for improving fertility.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Agriculture, Dairy & Animal Science
Thiago O. Cunha, Leah R. Statz, Rafael R. Domingues, Joao Paulo N. Andrade, Milo C. Wiltbank, Joao Paulo N. Martins
Summary: The objective of this study was to investigate the effects of inducing accessory corpus luteum on follicular and luteal dynamics in lactating Holstein cows. The results showed that inducing accessory corpus luteum not only altered the dynamics of follicles and corpus luteum but also deferred and prolonged the process of luteolysis.
JOURNAL OF DAIRY SCIENCE
(2022)
Review
Physiology
Corrine F. Monaco, John S. Davis
Summary: The corpus luteum is a transient endocrine gland in the ovary that produces progesterone for pregnancy. The formation and function of this gland rely on angiogenesis, which establishes the necessary blood flow and microvasculature. During regression, the corpus luteum undergoes structural changes and the blood vessels regress, impacting progesterone production. Factors released during luteolysis play a role in these processes, but the exact mechanisms remain unknown.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Jaya Bharati, N. H. Mohan, Satish Kumar, Jayashree Gogoi, Sai Kumar, Bosco Jose, Meeti Punetha, Sanjib Borah, Amit Kumar, Mihir Sarkar
Summary: RNA-Seq was used to identify differentially expressed genes (DEGs) in porcine corpus luteum (CL) during different physiological stages, revealing significant enrichment of gene functions in early, mid, and late stages. Apoptosis was most active in the regressed stage. Pathway analysis indicated lipid metabolism, translation, immune, and endocrine system pathways were associated with CL function. Gene network analysis identified novel hub genes AR, FOS, CDKN1A potentially regulating CL physiology.
Article
Cell Biology
Ewa Mlyczynska, Marta Kiezun, Patrycja Kurowska, Monika Dawid, Karolina Pich, Natalia Respekta, Mathilde Daudon, Edyta Rytelewska, Kamil Dobrzyn, Barbara Kaminska, Tadeusz Kaminski, Nina Smolinska, Joelle Dupont, Agnieszka Rak
Summary: In this review, we summarize the current knowledge about the expression and role of adipokines and neuropeptides in the physiological regulation of corpus luteum function, as well as their potential involvement in pathologies affecting the luteal cells that disrupt the estrous cycle.
Article
Agriculture, Dairy & Animal Science
M. L. Oliveira, B. P. Mello, A. M. Gonella-Diaza, S. C. Scolari, G. Pugliesi, T. Martins, I. R. Feltrin, R. Sartori, A. M. O. Canavessi, M. Binelli, C. M. B. Membrive
Summary: The study found that E2-induced PGF2 alpha release and luteolysis involve an unexpected and transient downregulation of components in the PGF2 alpha synthesis cascade, except for OXTR, which was upregulated. This suggests that E2 connects newly-synthesized OXTR to pre-existing cellular machinery to synthesize PGF2 alpha and cause luteal regression.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2022)
Article
Reproductive Biology
Corrine F. Monaco, Michele R. Plewes, Emilia Przygrodzka, Jitu W. George, Fang Qiu, Peng Xiao, Jennifer R. Wood, Andrea S. Cupp, John S. Davis
Summary: During the regression of the ovarian corpus luteum, fibroblasts are responsible for rapid matrix remodeling. However, little is known about the role of fibroblasts in the functional or regressing corpus luteum. In this study, it was found that FGF2 activates luteal fibroblasts, leading to collagen production and cell proliferation.
BIOLOGY OF REPRODUCTION
(2023)
Article
Biology
Meeti Punetha, Sai Kumar, Avishek Paul, Bosco Jose, Jaya Bharati, Arvind Sonwane, Jonathan A. Green, Kristin Whitworth, Mihir Sarkar
Summary: This study aimed to better understand the role of EGR1 in transactivation of TGF beta 1 during PGF2 alpha induced luteal regression using buffalo luteal cells. The results showed that EGR1 and TGF beta 1 mRNA expression were significantly upregulated at 12 hours post PGF2 alpha induction, and knocking out EGR1 did not modulate the expression of TGF beta 1. Additionally, the Caspase 3 mRNA expression was significantly increased in EGR1 KO luteal cells treated with PGF2 alpha compared to wild type luteal cells.
BIOLOGICAL RESEARCH
(2021)
Article
Reproductive Biology
Joao Paulo N. Martins, Melisa J. T. Acevedo, Christian G. Piterini, Thiago O. Cunha, J. Richard Pursley
Summary: The study tested the effect of low progesterone concentrations on pre-ovulatory follicle development in heifers and found that treatments affecting progesterone levels did not effectively control CL and follicle function, and therefore could not be used as a model to test the impact of progesterone on fertility parameters in Holstein heifers.
Article
Agriculture, Dairy & Animal Science
J. E. Gadsby, A. M. Tyson Nipper, H. A. Faircloth, M. D'Annibale-Tolhurst, J. Chang, P. W. Farin, I. M. Sheldon, D. H. Poole
REPRODUCTION IN DOMESTIC ANIMALS
(2017)
Article
Agriculture, Dairy & Animal Science
Rajagopal Sriperumbudur, Leah Zorrilla, John E. Gadsby
ANIMAL REPRODUCTION SCIENCE
(2010)
Article
Agriculture, Dairy & Animal Science
L. M. Zorrilla, M. S. Irvin, J. E. Gadsby
DOMESTIC ANIMAL ENDOCRINOLOGY
(2009)
Article
Agriculture, Dairy & Animal Science
I. M. Sheldon, S. B. Price, J. Cronin, R. O. Gilbert, J. E. Gadsby
REPRODUCTION IN DOMESTIC ANIMALS
(2009)
Article
Agriculture, Dairy & Animal Science
L. M. Zorrilla, M. A. D'Annibale, S. E. Swing, J. E. Gadsby
REPRODUCTION IN DOMESTIC ANIMALS
(2013)
Article
Veterinary Sciences
Theresa Beachler, Hanna Gracz, Nathan M. Long, Luke Borst, David Morgan, Amber Nebel, Natalie Andrews, Joanna Koipillai, Samantha Frable, Stasia Bembenek Bailey, Katelyn Ellis, Karen Von Dollen, Sara Lyle, John Gadsby, Christopher Scott Bailey
JOURNAL OF EQUINE VETERINARY SCIENCE
(2019)
Article
Agriculture, Dairy & Animal Science
J. E. Gadsby, S. Frandsen, J. Chang, B. Celestino, E. Tucker, D. H. Poole
DOMESTIC ANIMAL ENDOCRINOLOGY
(2020)
Article
Veterinary Sciences
Theresa M. Beachler, Hanna S. Gracz, David R. Morgan, Stasia A. Bembenek Bailey, Luke Borst, Katey E. Ellis, Karen A. Von Dollen, Sara K. Lyle, Amber Nebel, Natalie C. Andrews, Joanna Koipalli, John E. Gadsby, Christopher S. Bailey
Summary: Metabolomics was used to characterize plasma metabolomic profiles in healthy and induced placentitis mares. The study identified changes in metabolite concentrations post-infection that could potentially aid in early diagnosis of placentitis.
EQUINE VETERINARY JOURNAL
(2021)
Article
Agriculture, Dairy & Animal Science
J. Chang, S. Frandsen, J. E. Gadsby
DOMESTIC ANIMAL ENDOCRINOLOGY
(2017)
Article
Chemistry, Multidisciplinary
Hyun Suk Whang, Franck A. P. Vendeix, Hanna S. Gracz, John Gadsby, Alan Tonelli
PHARMACEUTICAL RESEARCH
(2008)
Article
Developmental Biology
U Boonyaprakob, JE Gadsby, V Hedgpeth, P Routh, GW Almond
Article
Agriculture, Dairy & Animal Science
ZP Ge, E Miller, W Nicholson, V Hedgpeth, J Gadsby
DOMESTIC ANIMAL ENDOCRINOLOGY
(2003)
Article
Developmental Biology
EA Miller, Z Ge, V Hedgpeth, JE Gadsby
Article
Developmental Biology
U Boonyaprakob, JE Gadsby, V Hedgpeth, P Routh, GW Almond
Article
Agriculture, Dairy & Animal Science
Mehak Nisar, Shafayat Ahmad Beigh, Abdul Qayoom Mir, Syed Ashaq Hussain, Aijaz Ahmad Dar, Iqbal Yatoo, Adil Mehraj Khan
Summary: This study aimed to evaluate the role of vitamin D in redox balance, insulin resistance, and its predicting value for subclinical pregnancy toxemia (SPT) in pregnant ewes. The results showed that ewes with insufficient vitamin D had significantly lower total antioxidant capacity (TAC) compared to ewes with sufficient vitamin D, and there were significant differences in metabolite concentrations between the two groups. Additionally, vitamin D was positively correlated with fructosamine, calcium, and TAC, and negatively correlated with non-esterified fatty acid (NEFA) and total oxidant status (TOS). Ewes with insufficient vitamin D had a higher risk of developing SPT two weeks prior to lambing and at lambing.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2024)
Article
Agriculture, Dairy & Animal Science
L. G. Gonzalez-Arostegui, A. Munoz-Prieto, C. P. Rubio, J. J. Ceron, L. Bernal, I. Rubic, V. Mrljak, J. C. Gonzalez-Sanchezc, A. Tvarijonaviciute
Summary: This study investigated changes in the saliva and serum proteome of dogs with hypothyroidism using TMT labelling and LC-MS/MS. The findings showed that this disease induces alterations in the saliva and serum proteome, reflecting physiological and pathological changes in the immune system, metabolism, skin, and coagulation. The downregulation of LDH in saliva may serve as a potential biomarker for canine hypothyroidism.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2024)
Article
Agriculture, Dairy & Animal Science
Camila R. C. Brito, Agatha D. Cordeiro, Pricila Baldessar, Carolini Schultz, Monike Quirino, Rafael R. Ulguim, Paulo B. D. Goncalves, Thomaz Lucia Jr, Ivan Bianchi, Bernardo G. Gasperin
Summary: This study tested synchronization protocols without progestogens during the luteal phase in gilts. The results showed that the protocols based on PGF and hCG were effective in reducing the interval between estrus in gilts. Additionally, the hCG2 protocol had negative effects on the number of piglets born alive and total litter size.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2024)
Article
Agriculture, Dairy & Animal Science
F. Frers, J. Delarocque, K. Feige, K. Huber, T. Warnken
Summary: This study aimed to investigate the relationship between insulin dysregulation and the expression and phosphorylation of key proteins in the insulin signaling cascade in different tissues of horses. The results showed enhanced phosphorylation of the insulin receptor and protein kinase B in response to stimulation, while phosphorylation of adenosine monophosphate protein kinase alpha decreased after glucose administration. Interestingly, horses with insulin dysregulation had significantly higher phosphorylation of mechanistic target of rapamycin in muscle tissue. These findings suggest that insulin signaling is maintained in horses, but post-receptor alterations may occur.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2024)
Article
Agriculture, Dairy & Animal Science
Patrice Dufour, Vincent Frisee, Goulven Rigaux, Flore Brutinel, Sophie Egyptien, Philippe Bossaert, Jessica Deleersnyder, Stefan Deleuze, Stephanie Peeters, Caroline Le Goff, Jerome Ponthier, Etienne Cavalier
Summary: This report describes the method of measuring progesterone and estrone-sulfate using Liquid Chromatography coupled with Mass Spectrometry in American Bison feces. This method provides an alternative to serum assay for studying the pregnancy-related evolution of these hormones in American Bison.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2024)