Article
Environmental Sciences
Xiyuan Xu, Shuai Wang, Chengxuan Li, Jingxi Li, Fenglei Gao, Li Zheng
Summary: Microplastics in the oceans are a new type of pollutant with complex biofilms. This study investigated the diversity and biological characteristics of quorum sensing bacteria in microplastic-associated biofilms, and found that these bacteria have strong biofilm-forming capacity and potential to exacerbate adverse environmental effects.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Review
Engineering, Environmental
Xueping Wang, Deshuang Yu, Guanghui Chen, Chengju Liu, Ao Xu, Zhihao Tang
Summary: This paper discusses the bacterial communication mechanism based on QS and the environmental factors affecting QS, compares QS inhibition strategies, and explores the role and prospects of QQ in long-term operation systems.
WATER ENVIRONMENT RESEARCH
(2021)
Article
Immunology
Pamela Ruiz, Daniela Sepulveda, Jose Miguel Vidal, Romina Romero, David Contreras, Javier Barros, Carlos Carrasco, Nathaly Ruiz-Tagle, Alex Romero, Homero Urrutia, Cristian Oliver
Summary: The study detected AHLs produced by P. salmonis using a fluorescent biosensor system and GC/MS. The results showed the presence of AHL-type molecule in both P. salmonis strains and suggested a possible alternate pathway for AHL synthesis. This report is the first to describe the type of AHLs produced by P. salmonis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Agricultural Engineering
Xiaojing Zhang, Bingbing Ma, Nan Zhang, Han Zhang, Yongpeng Ma, Yali Song, Hongzhong Zhang
Summary: This study investigated the regulatory effect of the external quorum sensing signals, N-dodecanoyl homoserine lactone (C12-HSL), on the CANON process in a membrane bioreactor. C12-HSL enhanced the activity of aerobic ammonia-oxidizing bacteria and improved ammonia monooxygenase activity, while suppressing anaerobic ammonia-oxidizing bacteria and limiting TN removal. Enzyme synthesis was enhanced, enabling the CANON system to resist high C12-HSL concentrations. C12-HSL was found to be profitable for aerobic ammonia oxidation and could be used to regulate nitrite production.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Xu Wang, Cancan Jiang, Danhua Wang, Yang Yang, Lijing Fan, Shengjun Xu, Xuliang Zhuang
Summary: Free nitrous acid (FNA) is widely used for wastewater management by altering sludge characteristic and function. This study investigated the role of collective behaviors in FNA treatment and revealed the impact of FNA on sludge morphology and composition.
Review
Biochemistry & Molecular Biology
Sania Sahreen, Hamid Mukhtar, Kalman Imre, Adriana Morar, Viorel Herman, Sundas Sharif
Summary: This review discusses the role of quorum sensing (QS) in biofilm formation and its importance in biological wastewater treatment. The different types of autoinducers and their mechanisms are explained, along with the methods of QS regulation and inhibition. The potential applications of QS-regulated biofilms in wastewater treatment are also summarized. The article concludes with an analysis of knowledge gaps in current research and proposed future study requirements.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Ecology
Mohammed Dwidar, Hyochan Jang, Naseer Sangwan, Wonsik Mun, Hansol Im, Sora Yoon, Sooin Choi, Dougu Nam, Robert J. Mitchell
Summary: The predatory bacterium Bdellovibrio bacteriovorus 109J preys on other Gram-negative bacteria to obtain nutrients for replication and survival. Two classes of bacterial signaling molecules, AHLs and DSF, were found to have different effects on the behavior and viability of B. bacteriovorus 109J. DSF significantly delayed predation and bdelloplast lysis, as well as reduced the motility of the cells. Additionally, DSF caused genome-wide changes in gene expression patterns, downregulating flagellum assembly genes and serine protease genes.
Article
Engineering, Environmental
Ying Xu, Thomas Curtis, Jan Dolfing, Yonghong Wu, Bruce E. Rittmann
Summary: Research findings suggest that the regulation of N-acyl-homoserine-lactones (AHLs) via quorum sensing signaling can optimize phosphorus (P) entrapment in microbial aggregates, promoting microbial growth and enhancing the community of polyphosphate-accumulating organisms (PAOs) to increase both intra-and extra-cellular P capture.
Article
Agricultural Engineering
Shamas Tabraiz, Burhan Shamurad, Evangelos Petropoulos, Marcos Quintela-Baluja, Alex Charlton, Jan Dolfing, Paul J. Sallis
Summary: The novel QQ strain Acinetobacter guillouiae ST01 showed high activity in reducing quorum sensing in a membrane bioreactor by decreasing AHL concentrations in the sludge and biofilm. It also significantly reduced polysaccharide and protein levels, leading to a decrease in fouling rates in the MBR. These results suggest that A. guillouiae ST01 is a promising candidate for biofouling control in wastewater treatment.
BIORESOURCE TECHNOLOGY
(2021)
Review
Biochemical Research Methods
Dhruv S. Gupta, Maushmi S. Kumar
Summary: Aquaculture industry is expanding rapidly, but it is facing challenges due to the emergence of antibiotic resistant pathogens. The usage of quorum sensing inhibitors has been proposed as a viable approach to tackle this problem.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)
Article
Multidisciplinary Sciences
Parker Smith, Martin Schuster
Summary: Quorum sensing is a widespread cell density-dependent signaling mechanism in bacteria. This study demonstrates the role of antiactivators in preventing self-sensing in the quorum sensing system of Pseudomonas aeruginosa.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Infectious Diseases
Lara Serrano-Aguirre, Rodrigo Velasco-Bucheli, Begona Garcia-Alvarez, Ana Saborido, Miguel Arroyo, Isabel de la Mata
Summary: This study identified a novel N-acylhomoserine lactone acylase from Actinoplanes utahensis, which can hydrolyze and synthesize various natural compounds, showing potential as a quorum quenching agent. This enzyme inhibits quorum sensing processes and can efficiently synthesize AHLs, highlighting its potential for the development of new antimicrobials.
Article
Environmental Sciences
Jie Cheng, Xin Su, Meng Liu, Zhijiang Lu, Jianming Xu, Yan He
Summary: This study investigated the effects of exogenous N-butanoyl homoserine lactone (C4-HSL), N-hexanoyl homoserine lactone (C6-HSL), and 3-oxo-hexanoyl homoserine lactone (3OC6-HSL) on the dechlorination of ?-hexachlorocyclohexane (?) and methane production. The results showed that exogenous C4-HSL and 3OC6-HSL increased cytochrome c production, enriched dechlorinators and electroactive bacteria, and accelerated dechlorination while inhibiting methane production. On the other hand, C6-HSL facilitated dechlorination and methane production by enhancing biofilm electroactivity and increasing membrane transportation.
ENVIRONMENTAL POLLUTION
(2023)
Review
Microbiology
Celia Mayer, Anabela Borges, Saskia-Camille Flament-Simon, Manuel Simoes
Summary: Escherichia coli is a common bacterium in the human and animal microbiota, but some strains can cause severe bacterial infections. It relies on a cell density-dependent communication system called quorum sensing (QS) to regulate virulence factors expression. Understanding the global QS network in E. coli is important for developing anti-virulence strategies.
FEMS MICROBIOLOGY REVIEWS
(2023)
Article
Microbiology
Nicole E. Smalley, Amy L. Schaefer, Kyle L. Asfahl, Crystal Perez, E. Peter Greenberg, Ajai A. Dandekar
Summary: In the bacterium Pseudomonas aeruginosa, quorum sensing (QS) can activate the expression of dozens to hundreds of genes. In this study, the researchers investigated how the size and content of the QS regulon can evolve during long-term growth of P. aeruginosa. They found that the QS regulon can undergo a reductive adaptation in response to continuous QS-dependent growth. These findings provide insights into the strain-to-strain variability in the size and content of the P. aeruginosa QS regulon.
Editorial Material
Integrative & Complementary Medicine
Sathiyanarayanan Lohidasan, K. R. Mahadik, Pulok K. Mukherjee, Subhash C. Mandal, Amit Kar
JOURNAL OF AYURVEDA AND INTEGRATIVE MEDICINE
(2023)
Review
Immunology
Md. Adil Shaharyar, Rudranil Bhowmik, Fahad A. Al-Abbasi, Shareefa A. AlGhamdi, Amira M. Alghamdi, Arnab Sarkar, Imran Kazmi, Sanmoy Karmakar
Summary: It is known that noncoding RNAs have important roles in both physiological and pathological processes. Recent findings suggest that modulating noncoding RNAs could provide therapeutic approaches. Noncoding RNAs consist of small RNAs, including long noncoding RNAs, siRNA, and microRNAs. MicroRNAs control gene expression patterns post-transcriptionally by binding to the 3' untranslated regions of target mRNA to inhibit or promote protein breakdown. On the other hand, long noncoding RNAs have a wider range of functions, including serving as molecular scaffolding, decoys, and epigenetic regulators. This article provides examples of long noncoding RNAs and microRNAs that could potentially serve as biomarkers for cardiovascular disease. The paper also highlights various RNA-based vaccine formulation strategies designed to target these biomarkers, which are either in the research pipeline or already in the global pharmaceutical market, and discusses the physiological hurdles that need to be overcome.
Article
Biochemical Research Methods
Shibu Narayan Jana, Subhadip Banerjee, Sayan Biswas, Dilip Sing, Amit Kar, Rajib Bandyopadhayay, Pallab K. Haldar, Nanaocha Sharma, Pulok K. Mukherjee
Summary: This study aims to validate and develop a simple, precise, and reproducible RP-HPLC and HPTLC methods for quantification of andrographolide in A. paniculata extracts. The validated methods confirmed different concentrations of andrographolide in the plant samples collected from different districts of West Bengal, India. This precise and reproducible method can be used for the quantification of andrographolide in kalmegh, according to the ICH guidelines recommendations.
JOURNAL OF CHROMATOGRAPHIC SCIENCE
(2023)
Article
Chemistry, Analytical
Barun Das Gupta, Amit Kar, Shyam Narayan, Chandreshwar Prasad Thakur, Pulok Kumar Mukherjee, Pallab Kanti Haldar
Summary: Ajwain, a high-value medicinal plant, is mainly distributed in India. It has various pharmacological activities such as antifungal, antimicrobial, and antitumor effects. The ethyl acetate fraction of Ajwain contains abundant phenolic compounds and flavonoids. Metabolite profiling using UPLC-QTOF-MS analysis identified 19 phytochemicals in the ethyl acetate fraction. A validated HPTLC method was developed to quantify the standard phytochemicals in the ethyl acetate fraction. This study provides a rapid and accurate method for the quality evaluation of Ajwain and its derived products.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Sushil K. Chaudhary, Amit Kar, Pardeep K. Bhardwaj, Nanaocha Sharma, Sarangthem Indira Devi, Pulok K. Mukherjee
Summary: A simple HPTLC method was developed for quantifying chlorogenic acid in Gynura cusimbua leaves extract. The method used silica gel 60F(254) pre-coated HPTLC plates with ethyl acetate-formic acid-acetic acid-water as the mobile phase. The method was validated for linearity, limits of detection and quantification, precision, accuracy, and robustness parameters. The amount of chlorogenic acid in the extract was found to be 3.37% w/w. The method can be used for standardization of G. cusimbua extract/formulation.
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC
(2023)
Article
Biochemistry & Molecular Biology
Nameirakpam Bunindro Singh, Moirangthem Lakshmipriyari Devi, Thokchom Biona, Nanaocha Sharma, Sudripta Das, Jharna Chakravorty, Pulok Kumar Mukherjee, Yallappa Rajashekar
Summary: This study investigates the chemical composition and antimicrobial activity of Artemisia vulgaris essential oil (AVEO) from the leaves of A. vulgaris grown in Manipur. The results show that AVEO has antimicrobial activity against both fungi and bacteria, making it a potential source for natural antimicrobial medications.
Article
Biochemistry & Molecular Biology
Soibam Thoithoisana Devi, Khaidem Devika Chanu, Nameirakpam Bunindro Singh, Sushil Kumar Chaudhary, Ojit Singh Keithellakpam, Kshetrimayum Birla Singh, Pulok K. Mukherjee, Nanaocha Sharma
Summary: Terminalia chebula, a medicinal plant that grows in Manipur, India, has been traditionally used for its laxative, anti-inflammatory, and remedy properties. The extracts obtained from this plant were analyzed and evaluated for their chemical constituents and anticancer activity against colon cancer cells. The study found significant variation in the composition of major compounds in the extracts, with 1,2,3-Benzenetriol being the most abundant. Marker compounds gallic acid and ellagic acid were also present in all extracts. The extracts showed strong antioxidant properties and cytotoxic effects on colon cancer cells.
Article
Biochemical Research Methods
Rupesh Banerjee, Barun Das Gupta, Amit Kar, Pardeep Kumar Bhardwaj, Nanaocha Sharma, Pallab Kanti Haldar, Rajib Bandyopadhyay, Pulok Kumar Mukherjee
Summary: This study evaluated the quality of pre- and post-marketed black rice samples using a validated high-performance thin layer chromatography method, and determined variations in total phenolics and total flavonoids with antioxidant potential. The results identified differences in quality among different varieties of black rice and demonstrated correlations between phenolic and flavonoid content and antioxidant potential. This study provides a useful method for the quality evaluation and nutritional authentication of black rice and its derived products.
PHYTOCHEMICAL ANALYSIS
(2023)
Correction
Pharmacology & Pharmacy
Sayantan Sengupta, Nipun Abhinav, Sabita Singh, Joytri Dutta, Ulaganathan Mabalirajan, Karthigeyan Kaliyamurthy, Pulok Kumar Mukherjee, Parasuraman Jaisankar, Arun Bandyopadhyay
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Analytical
Sushil Kumar Chaudhary, Amit Kar, Pardeep Kumar Bhardwaj, Nanaocha Sharma, Pulok K. Mukherjee
Summary: This study aimed to develop and validate a high-performance thin-layer chromatography method to quantify catechin in Potentilla fulgens roots extract and explore its antioxidant potential, acetylcholinesterase, and hydroxymethylglutaryl-coenzyme A reductase inhibition activity. The study found that the amount of catechin in Potentilla fulgens roots extract was 2.01% w/w. The extract showed significant scavenging potential and inhibited acetylcholinesterase and hydroxymethylglutaryl-coenzyme A reductase in a concentration-dependent manner. These results support the use of Potentilla fulgens as a folk medicine and suggest its potential as a rich source of natural antioxidants and enzyme inhibitory agents.
SEPARATION SCIENCE PLUS
(2023)
Article
Food Science & Technology
Wahengbam Deepanita Devi, Rajkumari Bonysana, Kokho Kapesa, Pulok Kumar Mukherjee, Yallappa Rajashekar
Summary: Insects are widely consumed and used for medicinal purposes around the world due to their medicinal value and therapeutic properties. Their fast reproduction rate makes them a sustainable source of therapeutic components. This traditional knowledge of entomotherapy can make a significant contribution to modern medicine. Additionally, the review highlights the traditional social beliefs associated with insects.
Article
Chemistry, Analytical
Sushil K. Chaudhary, Evanylla Kharlyngdoh, Jitendra K. Shukla, Pardeep K. Bhardwaj, Pulok K. Mukherjee
Summary: In this study, a simple, rapid and reproducible high-performance thin-layer chromatography method was developed for the quantitative estimation of genistein in Flemingia procumbens extracts. The method was validated and can be used for the standardization of the plant extract.
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC
(2023)
Article
Chemistry, Analytical
Sushil Kumar Chaudhary, K. Chandradev Sharma, Indira Sarangthem, Amit Kar, Pardeep Kumar Bhardwaj, Nanaocha Sharma, Pulok K. Mukherjee
Summary: The roots of Potentilla fulgens have been traditionally used for treating various diseases and are rich in polyphenols and flavonoids. High-performance liquid chromatography is a valuable technique for the quality control of herbal medicines, and a reversed-phase high-performance liquid chromatography method was developed and validated for the simultaneous determination of phenolic compounds in the extract of Potentilla fulgens roots. The results showed the presence of ellagic acid, rutin, and caffeic acid in the extract, while quercetin was not detected.
SEPARATION SCIENCE PLUS
(2023)
