Article
Oncology
Christopher J. Webster, Kelly L. George, Gillian R. Woollett
Summary: The principles of comparability assessments are globally accepted as sensitive and reliable tools for evaluating potential changes in biologics. Using these standards consistently for all biologics can improve market competitiveness and provide important efficiencies for regulators.
Article
Chemistry, Physical
Tobias Guckeisen, Saman Hosseinpour, Wolfgang Peukert
Summary: The change in protein secondary structure affects functionality and performance, with proteins responding differently to chemical denaturation in different environments. The liquid/air interface can either enhance or decrease protein conformation stability, while the presence of urea induces denaturation in bulk solution but may be prevented at the liquid/air interface.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yike Jiang, Weiping Jin, Jessica Li, Qingrong Huang
Summary: The study revealed that TF-3 primarily interacted with CPPs through hydrogen bonding, leading to the aggregation of CPPs. The binding of TF-3 to CPPs enhanced the cellular antioxidant activity of TF-3.
Article
Multidisciplinary Sciences
Nirupa Nagaratnam, Jose M. Martin-Garcia, Jay-How Yang, Matthew R. Goode, Gihan Ketawala, Felicia M. Craciunescu, James D. Zook, Manashi Sonowal, Dewight Williams, Thomas D. Grant, Raimund Fromme, Debra T. Hansen, Petra Fromme
Summary: Francisella tularensis is a highly infectious pathogen causing Tularemia. The lack of structural information on F. tularensis outer membrane protein A (FopA) hampers its therapeutic interventions against Tularemia. In this study, we successfully determined the structure and organization of membrane localized mature FopA, paving the way for understanding its role in pathogenesis and developing anti-tularemia therapeutics.
Article
Chemistry, Analytical
Hum Bahadur Lamichhane, Terence G. Henares, Mark J. Hackett, Damien W. M. Arrigan
Summary: Understanding protein interactions at interfaces, particularly using experimental methods such as ITIES, can provide insights into how protein structure is influenced by interactions at interfaces. In this study, the destabilization of native insulin secondary structure and the promotion of helix secondary structures at interfaces were found to be driven by protein-interface interactions, highlighting the potential of ITIES as a tool for studying protein structure and interactions.
ANALYTICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Martin Eduardo Villanueva, Maria Angel da Silva, Jose Luis Barra, Guillermo Gabriel Montich, Ismael Dario Bianco, Silvina R. Salinas
Summary: This study investigated the biophysical properties of recombinant chi18-5. The conformation of chi18-5 was found to change near its isoelectric point, and with increasing temperature, there was an increase in beta-sheet secondary structure and a decrease in alpha-helix. Additionally, at pH below the isoelectric point, chi18-5 exhibited a less compact structure, which may have implications for substrate interaction and enzyme activity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jose L. Neira, Salome Araujo-Abad, Ana Camara-Artigas, Bruno Rizzuti, Olga Abian, Ana Marcela Giudici, Adrian Velazquez-Campoy, Camino de Juan Romero
Summary: This study investigates the biophysical features of PADI4 and its altered expression in cancer cells. The researchers found different expression patterns and subcellular localizations of PADI4 in various cancer cell lines, and its potential role in tumorigenesis by regulating p53 expression.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Kavyashree Sakharayapatna Ranganatha, Ashapogu Venugopal, Dinesh Kumar Chinthapalli, Rajagopal Subramanyam, Siva Kumar Nadimpalli
Summary: In this study, a low molecular weight acidic alpha-galactosidase (CaG) was identified from custard apple seeds and purified using various chromatographic techniques. The purified CaG was found to be a heterodimer with specific kinetic parameters and secondary structures identified through CD spectral analysis. These findings provide insights into the primary sequence and glycan structures of CaG, defining its potential physiological roles in plants and industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Ingrid Contardo, Fanny Guzman, Javier Enrione
Summary: This study investigated the impact of simulated salivary alterations on the conformational properties and secondary structure of chickpea protein isolate (CPI) in elderly and adult subjects. The results showed that the ordered secondary protein structure was significantly affected in the elderly population, with a decrease in alpha-helix and an increase in unordered random coil state. These findings could be attributed to an increase in electrolytes in the salivary composition. The study provides meaningful insights into understanding how alterations in the elderly oral system affect protein conformation and may contribute to improving the understanding of plant-based protein digestibility.
Article
Food Science & Technology
Gbemisola J. Fadimu, Harsharn Gill, Asgar Farahnaky, Tuyen Truong
Summary: The effects of high-power and low-frequency ultrasound pretreatment, as well as different durations and proteases, on lupin protein hydrolysates were examined in this study. The results showed changes in structural characteristics, antioxidant activities, and physicochemical properties of the hydrolysates. Ultrasound treatment had a noticeable impact on the protein structure and antioxidant activity of the hydrolysates.
FOOD AND BIOPROCESS TECHNOLOGY
(2021)
Article
Biology
Torleif Tollefsrud Gjolberg, Rahel Frick, Simone Mester, Stian Foss, Algirdas Grevys, Lene Stokken Hoydahl, Oystein Kalsnes Jorstad, Tilman Schlothauer, Inger Sandlie, Morten C. Moe, Jan Terje Andersen
Summary: The analysis of clinically approved antibody-based therapeutics reveals that different structural designs have distinct biophysical properties affecting FcRn binding, intracellular transport, and plasma half-life. Understanding the impact of different features of antibody-based therapeutics on FcRn binding and transport is crucial for optimizing cellular sorting and plasma half-life. This study compares the FcRn engagement of IgG1 Fc fragment to clinically relevant IgGs and receptor domain Fc fusions, binding to VEGF or TNF-alpha, and provides insights into their intracellular accumulation, plasma half-life, and cellular transportation.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anurag S. Rathore, Srishti Joshi, Neh Nupur, Nikita Saxena, Sanghati Bhattacharya, Souhardya Roy
Summary: Biosimilar manufacturers use a chemometric approach to assess biosimilarity, aiming to simplify assessment and eliminate individual bias. This approach collates chemical information into a single plot for quantitative assessment. It allows for numerical grading of similarity for biosimilars and can be easily incorporated within the existing regulatory framework.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Jeffrey A. Julien, Martin G. Fernandez, Katrina M. Brandmier, Joshua T. Del Mundo, Carol M. Bator, Lucie A. Loftus, Esther W. Gomez, Enrique D. Gomez, Kerney Jebrell Glover
Summary: Nanodiscs, disc-shaped entities containing a central lipid bilayer encased by amphipathic helices, have become a leading membrane mimic. The current method of forming nanodiscs involves a time-consuming process of dialysis and/or incubation with sorptive beads to remove detergent. In contrast, the method described in this study offers a rapid procedure using Sephadex (R) G-25 resin, attracting time-sensitive and high-throughput experiments.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Chemistry, Multidisciplinary
Ayonbala Baral, Lakkoji Satish, Subrat K. Padhy, Dipti P. Das, Shaohua Ju, Malay K. Ghosh
Summary: This work describes the synthesis of lithium-manganese dioxide from natural manganese resources, and investigates the molecular interactions of the synthesized nanocomposites with lysozyme. The study highlights the importance of understanding the fate of nanocomposites in biological applications, and provides insights into the potential use of different nanocomposites for further biological studies.
JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS
(2021)
Article
Chemistry, Physical
Anna Tanuja Safala Bodapati, Ragaiahgari Srinivas Reddy, Kandikonda Lavanya, Shravya Rao Madku, Bijaya Ketan Sahoo
Summary: The present study investigates the interaction between the new generation proton pump inhibitor dexlansoprazole (DLP) and human serum albumin (HSA). Fluorescence and UV-visible spectroscopy were used to quantify the interaction and determine parameters such as quenching constant, binding constant, and thermodynamic parameters. The results show that DLP and HSA interact with a hypochromic effect observed in absorption studies. The binding constants obtained from fluorescence studies indicate a collisional-type quenching mechanism. The study also confirms the binding of DLP in subdomain IIA of HSA through site marker competitive displacement studies and molecular docking.
JOURNAL OF MOLECULAR LIQUIDS
(2023)