Review
Biochemistry & Molecular Biology
Laura Vanderwaeren, Ruveyda Dok, Karin Voordeckers, Sandra Nuyts, Kevin J. Verstrepen
Summary: Yeast is an important enzyme source for bread and beer production and is an ideal model organism for studying eukaryotic cell biology and genetics. This review discusses the characteristics that make yeast such a widely used model organism, with a focus on the DNA damage response pathway as an example of its contribution to elucidating highly conserved biological processes. Differences in the DNA damage response of yeast and humans are also highlighted, along with the challenges of using yeast as a model system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Momoko Hayashida, Wataru Nomura, Atsushi Shiojiri, Yoshiharu Inoue
Summary: In Saccharomyces cerevisiae, the duplication and proper positioning of spindle pole bodies (SPBs) during cell cycle progression is crucial for nuclear division. This study found that the metabolite methylglyoxal (MG) derived from glycolysis perturbs the positioning of SPBs by affecting the asymmetric localization of the protein Kar9. It was also discovered that MG activates the DNA damage checkpoint pathway, which further disrupts the proper positioning of SPBs.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Li Cheng, Xuetong Yue, Zhaoyu Qin, Xiaogang Sun, Fuchu He, Junbiao Dai, Chen Ding
Summary: This study reports a genome-wide quantitative proteomic system that uses unique code peptides to achieve precise protein quantification. By systematically tagging and decoding 40 yeast proteins using mass spectrometry, the researchers successfully recovered all code peptides with a large and consistent quantitative dynamic range. Additionally, they verified the alterations in glucose and galactose metabolism pathways in yeast under different carbon source conditions.
Article
Microbiology
Li Fan, Wenqing Zhang, Josephine Rybchuk, Yu Luo, Wei Xiao
Summary: DNA-damage tolerance is a mechanism that allows eukaryotes to bypass replication-blocking lesions and continue DNA synthesis. In this study, a mutant with a pol30-A171D mutation was found to rescue DNA-damage sensitivity in a srs2-dependent and PCNA sumoylation-independent manner, providing insights into the molecular mechanism of the interaction between PCNA and Srs2.
Article
Biology
Mark C. Johnson, Geylani Can, Miguel Monteiro Santos, Diana Alexander, Philip Zegerman
Summary: This study reveals that Rad53 phosphorylates the same substrates throughout the cell cycle, not just in S-phase, suggesting its roles beyond S-phase. By inhibiting 51d3 and Dbf4, Rad53 limits re-replication in G2/M, preventing gene amplification, and inhibiting these substrates in G1 prevents premature initiation at the G1/S transition. This redefinition of the 'S-phase checkpoint' has implications for understanding checkpoint function in cancers without proper cell cycle controls.
Article
Microbiology
Ji Eun Choi, Seo-Hee Heo, Woo-Hyun Chung
Summary: In this study, it was found that Yap1 is a key gene for caffeine tolerance in yeast cells, enhancing cell resistance to high concentrations of caffeine by regulating oxidative stress response. Additionally, caffeine can reduce the generation of intracellular reactive oxygen species and affect mutation rate and DNA repair.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Daria Avdanina, Alexander Zghun
Summary: The manufacturing of sherry wines involves the use of specialized yeast strains for biological aging, resulting in unique chemical composition and sensory properties. Modern genetic and biotechnological methods contribute to breeding high-quality yeast strains.
FERMENTATION-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Elena Galati, Maria C. Bosio, Daniele Novarina, Matteo Chiara, Giulia M. Bernini, Alessandro M. Mozzarelli, Maria L. Garcia-Rubio, Belen Gomez-Gonzalez, Andres Aguilera, Thomas Carzaniga, Marco Todisco, Tommaso Bellini, Giulia M. Nava, Gianmaria Frige, Sarah Sertic, David S. Horner, Anastasia Baryshnikova, Caterina Manzari, Anna M. D'Erchia, Graziano Pesole, Grant W. Brown, Marco Muzi-Falconi, Federico Lazzaro
Summary: This study identifies VID22 as a novel regulator of genome integrity in yeast cells, involved in DNA double-strand break repair and protection of G4-DNA structures. Loss of VID22 leads to increased levels of endogenous DNA damage and hypersensitivity to G4-stabilizing ligands.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Cell Biology
Candice Qiu Xia Yam, Hong Hwa Lim, Uttam Surana
Summary: The article mainly discusses the role of the DNA damage checkpoint in cell cycle regulation, and how cells repair and continue cell cycle progression when damaged. It also mentions a potential unusual cellular response that cells may take when corrective measures fail.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ruofan Yu, Xiaohua Cao, Luyang Sun, Jun-Yi Zhu, Brian M. Wasko, Wei Liu, Emeline Crutcher, Haiying Liu, Myeong Chan Jo, Lidong Qin, Matt Kaeberlein, Zhe Han, Weiwei Dang
Summary: Histone acetylations serve as important epigenetic markers that regulate transcriptional activation in response to metabolic changes and stresses. In this study, the inactivation of the HDA complex was found to activate longevity and confer resistance to stress through trehalose metabolism. This longevity effect is independent of the Cyc8-Tup1 repressor complex and is evolutionarily conserved.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Tyler W. Doughty, Rosemary Yu, Lucy Fang- Chao, Zhongjun Qin, Verena Siewers, Jens Nielsen
Summary: The single chromosome strain exhibited poorer growth characteristics on non-fermentable carbon sources due to sirtuin-mediated silencing. Nicotinamide was able to rescue the diminished growth of the strain on ethanol.
Review
Biotechnology & Applied Microbiology
Charlotte C. Koster, Eline D. Postma, Ewout Knibbe, Celine Cleij, Pascale Daran-Lapujade
Summary: Synthetic Genomics focuses on designing chromosomes and genomes, and offers new methods for studying biology and constructing synthetic cell factories. The current limitation in Synthetic Genomics is the inability to synthesize DNA molecules longer than a few hundred base pairs, while the smallest self-replicating cell genome is several hundred thousand base pairs. This calls for the development of methods to assemble small DNA fragments into large molecules. Saccharomyces cerevisiae, a unicellular eukaryote, has been instrumental in DNA assembly and has a crucial role in the future development of Synthetic Genomics.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cell Biology
James E. Haber
Summary: Miroslav Radman's visionary ideas have had a significant impact on our study of the repair of broken eukaryotic chromosomes. For over 35 years, our lab has been investigating various aspects of chromosomal break repair in yeast, sometimes even advancing some of Radman's ideas further.
Article
Biochemistry & Molecular Biology
Anna S. Zhuk, Artem G. Lada, Youri I. Pavlov
Summary: Baker's yeast, S. cerevisiae, is widely used as a model organism for studying genome stability mechanisms. The genetic differences among commonly used yeast strains affect DNA replication, repair, and recombination processes. This study compared the genomic DNA sequence variation in five yeast strains used for studying these processes and found significant differences, even in closely related strains. These findings highlight the importance of considering strain polymorphisms when interpreting genome stability studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mrinal Sharma, Prranshu Yadav, Ankita Doshi, Hemang D. Brahmbhatt, C. Ratna Prabha
Summary: The study examined the effects of six double mutants derived from the ubiquitin mutant UbEP42 on cell functions, finding that the double mutation L50P-I61T had the most detrimental impact. Overall, these double mutations made cells more sensitive, and the negative effects under certain conditions could be compensated for by each other.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Meeting Abstract
Oncology
Celine Clemenson, Cyrus Chargari, Michele Mondini, Charles Ferte, Winchygn Liu, Mike Burbridge, Valerie Cattan, Anne Jacquet-Bescond, Eric Deutsch
Article
Oncology
Michele Mondini, Mevyn Nizard, Thi Tran, Laetitia Mauge, Mauro Loi, Celine Clemenson, Delphine Dugue, Pierre Maroun, Emilie Louvet, Julien Adam, Cecile Badoual, Dominique Helley, Estelle Dransart, Ludger Johannes, Marie-Catherine Vozenin, Jean-Luc Perfettini, Eric Tartour, Eric Deutsch
MOLECULAR CANCER THERAPEUTICS
(2015)
Article
Biotechnology & Applied Microbiology
Ingrid Fumagalli, Delphine Dugue, Jean Emmanuel Bibault, Celine Clemenson, Marie Catherine Vozenin, Michele Mondini, Eric Deutsch
ONCOTARGETS AND THERAPY
(2015)
Article
Oncology
Celine Clemenson, Cyrus Chargari, Winchygn Liu, Michele Mondini, Charles Ferte, Mike F. Burbridge, Valerie Cattan, Anne Jacquet-Bescond, Eric Deutsch
MOLECULAR CANCER THERAPEUTICS
(2017)
Article
Multidisciplinary Sciences
Bertrand Routy, Emmanuelle Le Chatelier, Lisa Derosa, Connie P. M. Duong, Maryam Tidjani Alou, Romain Daillere, Aurelie Fluckiger, Meriem Messaoudene, Conrad Rauber, Maria P. Roberti, Marine Fidelle, Caroline Flament, Vichnou Poirier-Colame, Paule Opolon, Christophe Klein, Kristina Iribarren, Laura Mondragon, Nicolas Jacquelot, Bo Qu, Gladys Ferrere, Celine Clemenson, Laura Mezquita, Jordi Remon Masip, Charles Naltet, Solenn Brosseau, Coureche Kaderbhai, Corentin Richard, Hira Rizvi, Florence Levenez, Nathalie Galleron, Benoit Quinquis, Nicolas Pons, Bernhard Ryffel, Veronique Minard-Colin, Patrick Gonin, Jean-Charles Soria, Eric Deutsch, Yohann Loriot, Francois Ghiringhelli, Gerard Zalcman, Francois Goldwasser, Bernard Escudier, Matthew D. Hellmann, Alexander Eggermont, Didier Raoult, Laurence Albiges, Guido Kroemer, Laurence Zitvogel
Review
Pharmacology & Pharmacy
Cyrus Chargari, Celine Clemenson, Isabelle Martins, Jean-Luc Perfettini, Eric Deutsch
DRUG RESISTANCE UPDATES
(2013)
Article
Oncology
Celine Clemenson, Erwan Jouannot, Ana Merino-Trigo, Chantal Rubin-Carrez, Eric Deutsch
INVESTIGATIONAL NEW DRUGS
(2013)
Article
Oncology
S. Gachet, E. Genesca, D. Passaro, M. Irigoyen, H. Alcalde, C. Clemenson, S. Poglio, F. Pflumio, A. Janin, C. Lasgi, S. Dodier, M. Soyer, G. Dumenil, J. Ghysdael
Meeting Abstract
Oncology
Cyrus Chargari, Celine Clemenson, Michele Mondini, Charles Ferte, Anne Jacquet-Bescond, Valerie Cattan, Stephane Depil, Eric Deutsch
MOLECULAR CANCER THERAPEUTICS
(2013)
Article
Oncology
Charles Ferte, Yohann Loriot, Celine Clemenson, Frederic Commo, Andrea Gombos, Jean-Emmanuel Bibault, Ingrid Fumagalli, Saad Hamama, Nathalie Auger, Benoit Lahon, Cyrus Chargari, Julien Calderaro, Jean-Charles Soria, Eric Deutsch
MOLECULAR CANCER THERAPEUTICS
(2013)
Article
Oncology
Michele Mondini, Pierre-Louis Loyher, Pauline Hamon, Marine Gerbe de Thore, Marie Laviron, Kevin Berthelot, Celine Clemenson, Benoit L. Salomon, Christophe Combadiere, Eric Deutsch, Alexandre Boissonnas
CANCER IMMUNOLOGY RESEARCH
(2019)
Article
Oncology
Celine Clemenson, Winchygn Liu, Denis Bricout, Loren Soyez-Herkert, Cyrus Chargari, Michele Mondini, Raphael Haddad, Xiuping Wang-Zhang, Laurent Benel, Christian Bloy, Eric Deutsch
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2019)
Article
Oncology
Vanessa Tran Chau, Winchygn Liu, Marine Gerbe de Thore, Lydia Meziani, Michele Mondini, Mark J. O'Connor, Eric Deutsch, Celine Clemenson
BRITISH JOURNAL OF CANCER
(2020)
Article
Oncology
Lydia Meziani, Marine Gerbe de Thore, Pauline Hamon, Sophie Bockel, Ruy Andrade Louzada, Celine Clemenson, Raphael Corre, Wincgygn Liu, Corinne Dupuy, Michele Mondini, Eric Deutsch
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2020)
Meeting Abstract
Oncology
Michele Mondini, Pierre L. Loyher, Kevin Berthelot, Celine Clemenson, Alexandre Boissonnas, Eric Deutsch
Article
Genetics & Heredity
Marit A. E. van Bueren, Aniek Janssen
Summary: Eukaryotic nuclei rely on multiple repair pathways to accurately repair DNA damage, particularly in chromatin domains enriched for repetitive DNA sequences. Tailored repair mechanisms are necessary to maintain genome stability in these domains.