Article
Plant Sciences
Hee Tae Lee, Hyo-Young Park, Keh Chien Lee, Jeong Hwan Lee, Jeong-Kook Kim
Summary: We investigated the transcriptomic changes in shoot apices during floral transition in Arabidopsis mutants of splicing factors AtU2AF65a and AtU2AF65b. AtU2AF65a mutants showed delayed flowering, while AtU2AF65b mutants exhibited accelerated flowering. RNA-seq analysis revealed that AtU2AF65a mutants had more differentially expressed genes compared to AtU2AF65b mutants. The gene FLOWERING LOCUS C (FLC), a major floral repressor, was significantly up- or down-regulated in the mutants. Additionally, the expression and alternative splicing patterns of several FLC upstream regulators were altered in the mutants. Our findings suggest that AtU2AF65a and AtU2AF65b splicing factors modulate FLC expression by affecting the expression or AS patterns of FLC upstream regulators, leading to different flowering phenotypes.
Review
Biochemistry & Molecular Biology
Rahaba Marima, Rodney Hull, Georgios Lolas, Konstantinos N. Syrigos, Minah Kgoebane-Maseko, Andreas Martin Kaufmann, Zodwa Dlamini
Summary: Cervical cancer is a major public health issue in low-to-middle-income countries, with human papillomavirus infection being the main cause. HIV positive women are at higher risk of HPV and cervical cancer, particularly in African countries. While studies have shown a relationship between HPV, HIV, and cervical cancer, there is still a gap in understanding the precise nature of this tripartite association.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
A. S. Venkataramany, K. M. Schieffer, K. Lee, C. E. Cottrell, P. Y. Wang, E. R. Mardis, T. P. Cripe, D. S. Chandler
Summary: Alternative splicing plays a critical role in the formation and growth of pediatric cancers. This review highlights the various splice variants and affected genes in different types of cancers. Understanding the mechanisms of disease-inducing splicing events can contribute to the development of novel therapeutics.
ANNALS OF ONCOLOGY
(2022)
Article
Genetics & Heredity
Jing Song, Jia Liu, Dekang Lv, Xuan Meng, Xiaodong Li
Summary: This study identified survival-associated AS signatures in Lung Adenocarcinoma (LUAD) and established a new combined prognostic model with strong predictive properties. The research also revealed a splicing network between AS and SFs, as well as a possible mechanism for tumors to evade immune recognition by decreasing expression of antigen presentation genes. Additionally, several small-molecule drugs were provided as potential inhibitors of LUAD, with NVP-AUY922 showing promising results in prolonging patient survival.
FRONTIERS IN GENETICS
(2021)
Review
Immunology
Pingping Ren, Luying Lu, Shasha Cai, Jianghua Chen, Weiqiang Lin, Fei Han
Summary: Alternative splicing is a complex regulatory mechanism that affects a large percentage of genes and organs, aberrant splicing can lead to various diseases, though its association with autoimmune diseases requires further examination.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Agronomy
Xiaojiao Hu, Hongwu Wang, Kun Li, Xiaogang Liu, Zhifang Liu, Yujin Wu, Shuqiang Li, Changling Huang
Summary: The study reveals a large number of significant differential alternative splicing events in the maize hybrid ZD808 compared to its parents, with NG5-dominant events playing a prevalent role and mainly involved in regulating genes associated with carbon/nitrogen metabolism and cell division processes.
Article
Oncology
Yao-Jie Pan, Fu-Chun Huo, Meng-Jie Kang, Bo-Wen Liu, Meng-Di Wu, Dong-Sheng Pei
Summary: SRSF11 is found to be overexpressed in colorectal cancer (CRC) and associated with poor prognosis. It plays a pro-metastatic role in both in vitro and in vivo settings. The study highlights the relationship between SRSF11-regulated splicing and CRC metastasis, suggesting a potential therapeutic target and prognostic biomarker in CRC.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Plant Sciences
Marta Budziszewska, Przemyslaw Wieczorek
Summary: Research described the genetic variability of necrosis-inducing ToTV-Wal'17 derived from ToTV-Wal'03 after long-term passages in plants, including single nucleotide substitutions, amino acid changes in viral polyproteins, and a shorter 3'UTR compared to Wal'03. Sequence analyses revealed that the 3'UTR of RNA1 of ToTV-Wal'17 is the most divergent among all previously described European isolates.
Article
Microbiology
Han Xiao, Emanuel Wyler, Miha Milek, Bastian Grewe, Philipp Kirchner, Arif Ekici, Ana Beatriz Oliveira Villela Silva, Doris Jungnickl, Florian Full, Marco Thomas, Markus Landthaler, Armin Ensser, Klaus Ueberla
Summary: The HIV-1 Rev protein is crucial for nuclear export of unspliced and incompletely spliced HIV-1 RNAs. Depletion of proteins like CRNKL1 can significantly enhance levels of unspliced HIV-1 RNA in the cytoplasm, potentially revealing a cellular mechanism exploited by HIV-1 to regulate its complex splicing pattern.
Article
Biochemistry & Molecular Biology
Victor Olmos, Evrett N. Thompson, Neha Gogia, Kimberly Luttik, Vaishnavi Veeranki, Luhan Ni, Serena Sim, Kelly Chen, Diane S. Krause, Janghoo Lim
Summary: This study reveals that mutant ATAXIN-1 can lead to diverse splicing events and contribute to the pathogenesis of spinocerebellar ataxia type 1. The researchers identify Rbfox1 as a mediator of mutant ATAXIN-1's effect on misregulated alternative splicing and demonstrate that manipulation of Rbfox1 expression can modify neurodegenerative phenotypes in a Drosophila model. These findings provide valuable insights into the molecular mechanisms and potential therapeutic strategies for spinocerebellar ataxia type 1.
HUMAN MOLECULAR GENETICS
(2023)
Article
Oncology
Yuchen Shen, Xinyu Li, Deming Wang, Liming Zhang, Xiao Li, Lixin Su, Xindong Fan, Xitao Yang
Summary: This study aimed to identify a novel prognostic biomarker for head and neck squamous cell carcinoma (HNSCC) based on tumor immunology-related alternative splicing (AS). Through the establishment of an AS-related risk model, the researchers found that Collagen Type III Alpha 1 Chain (COL3A1) is a reliable biomarker for predicting the prognosis of HNSCC patients. COL3A1 expression levels were elevated in HNSCC tissues compared to normal tissues and were associated with HNSCC differentiation and T stage. Additionally, COL3A1 was found to be better at evaluating immune cell infiltrations and activities in HNSCC lesions compared to the risk model.
Review
Genetics & Heredity
Ling Li, Jinxia Zheng, Sebastian Oltean
Summary: This article discusses the role of alternative splicing (AS) in the regulation of epithelial-mesenchymal transition (EMT) and suggests that reversing or inhibiting EMT may provide new therapeutic strategies for suppressing tumor cell migration and metastasis.
Review
Virology
Ruixue Li, Shenyan Gao, Huayuan Chen, Xiaozhan Zhang, Xia Yang, Jun Zhao, Zeng Wang
Summary: Since invasion, there is a continuous battle between host and virus for cellular resources during infection. Alternative splicing (AS), a mechanism to increase protein diversity, plays a crucial role in regulating viral protein expression and host immune response against viral infection. This review provides insights into host-virus interactions, viral pathogenesis, and potential targets for antiviral drug development.
Article
Biochemistry & Molecular Biology
Hade Ramos, Anne Monette, Meijuan Niu, Aldo Barrera, Brenda Lopez-Ulloa, Yazmin Fuentes, Paola Guizar, Karla Pino, Luc DesGroseillers, Andrew J. Mouland, Marcelo Lopez-Lastra
Summary: This study provides evidence that Staufen1 plays a novel role as an ITAF promoting HIV-1 vRNA IRES activity, by associating with both the HIV-1 vRNA and the Gag protein, and affecting HIV-1 translation initiation in a cap- or an IRES-dependent mechanism. SiRNA-mediated depletion of Staufen1 decreases HIV-1 vRNA translation, while cDNA-mediated overexpression of Staufen1 acutely regulates HIV-1 IRES activity. Additionally, the interaction between Staufen1 and HIV-1 vRNA is necessary for enhancing HIV-1 IRES activity.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Immunology
Luke Adam White, Thomas C. Bisom, Hunter L. Grimes, Miyuki Hayashi, Jean-Marc Lanchy, J. Stephen Lodmell
Summary: The study reveals that Rift Valley fever virus infection leads to alternative splicing of TR2-beta, which decreases cellular immune response. Additionally, TR2-beta is identified as a key regulator of RIOK3, contributing to its antiviral effect against Rift Valley fever virus.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Public, Environmental & Occupational Health
Nicole M. Coombs, Joanne E. Porter, Michael Barbagallo, Virginia Plummer
Summary: This article conducted a survey on the extent, range, and nature of public health education provided by emergency nurses and found that there is limited research in this area. Emergency nurses need to make use of every emergency admission as a "teachable moment" to provide preventative education and improve health outcomes while reducing the demand on the healthcare system.
PATIENT EDUCATION AND COUNSELING
(2022)
Review
Health Policy & Services
Elizabeth M. Miller, Joanne E. Porter, Michael S. Barbagallo
Summary: This literature review explores how truth disclosure is experienced by health professionals, patients, and families in the healthcare setting when delivering bad news. The study identified enablers for breaking bad news and the concept of truth avoidance/disclosure. The findings highlight the importance of truth disclosure in communication dynamics when breaking bad news.
PALLIATIVE & SUPPORTIVE CARE
(2022)
Review
Public, Environmental & Occupational Health
Elizabeth M. Miller, Joanne E. Porter, Michael S. Barbagallo
Summary: The aim of the study was to review how the physical hospital environment affects palliative patients and their families. The findings suggest that despite a preference for dying at home, most palliative patients end up receiving end-of-life care in hospital. Improving the hospital environment to meet the needs of patients and families is crucial for providing a safe, private, customizable space for reflection and sharing with loved ones.
HERD-HEALTH ENVIRONMENTS RESEARCH & DESIGN JOURNAL
(2022)
Review
Environmental Sciences
Elissa Dabkowski, Joanne E. Porter, Michael S. Barbagallo, Valerie Prokopiv, Megan R. Jackson
Summary: The need for continued research into suicide prevention strategies is undeniable, especially in rural and regional areas where there is a higher risk of dying by suicide. This scoping review explored literature on community-based suicide prevention initiatives in rural and regional Australia and found a lack of research in this area. Further evaluation and tailoring of programs is needed to ensure effective suicide prevention for these communities.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Review
Education & Educational Research
Joanne E. Porter, Elissa Dabkowski, Val Prokopiv, Karen Missen, Michael Barbagallo, Michelle James
Summary: This review examines the impact of physical literacy programs designed for two- to five-year-old preschool children. The findings suggest that early childhood physical literacy programs provide holistic benefits, though further research is needed in an Australian context. Families and community members in the early childhood sector could benefit from additional education and training to enhance awareness of physical literacy.
AUSTRALASIAN JOURNAL OF EARLY CHILDHOOD
(2023)
Article
Emergency Medicine
Nicole M. Coombs, Joanne E. Porter, Michael Barbagallo, Virginia Plummer
Summary: This study aims to explore the practice and attitudes of Australian emergency nurses in providing public health messages during disasters. The study found that there are barriers, such as negative attitudes, lack of confidence, time, policy, and training, that hinder the provision of public health messages in the emergency setting. Therefore, it is necessary to address these barriers to ensure that nurses are confident and supported in their public health messaging practice during disasters.
AUSTRALASIAN EMERGENCY CARE
(2023)
Article
Health Policy & Services
Elizabeth M. Miller, Joanne E. Porter, Michael S. Barbagallo
Summary: This study explores how the therapeutic nature of the environment affects patients and their families when receiving bad news. Through the Therapeutic Landscapes theoretical framework, the study found that language and environment had an impact on the experience of receiving bad news. These findings are significant for informing practice, future policy, and the design and aesthetics of environments where bad news is delivered.
PALLIATIVE & SUPPORTIVE CARE
(2023)
Article
Rehabilitation
Michelle H. James, Valerie Prokopiv, Michael S. Barbagallo, Joanne E. Porter, Nicholas Johnson, Jan Jones, Tanisha Smitherson
Summary: This study aims to explain why Aboriginal and Torres Strait Islander People with a disability continue to face barriers to service engagement. The meta-synthesis of existing literature reveals that disability services do not adequately consider the cultural needs of this population or communicate in a culturally appropriate manner. More efforts are needed to align disability services with culturally appropriate support and improve health outcomes.
DISABILITY AND REHABILITATION
(2023)
Article
Emergency Medicine
Nicole M. Coombs, Joanne E. Porter, Michael Barbagallo
Summary: This study investigates the perceptions and experiences of Australian emergency nurses regarding preventative messaging provided during disasters as well as the governance and processes supporting this practice. The findings highlight the importance of nurse confidence in delivering preventative messages and reveal a lack of preparation and support within departments.
AUSTRALASIAN EMERGENCY CARE
(2023)
Article
Public, Environmental & Occupational Health
Elizabeth M. Miller, Joanne E. Porter, Michael S. Barbagallo
Summary: The study aims to explore regional nurses' perspectives on how bad news is delivered and the physical, natural, social, and symbolic environments where these conversations occur.
HERD-HEALTH ENVIRONMENTS RESEARCH & DESIGN JOURNAL
(2023)
Review
Psychology, Multidisciplinary
Elissa Dabkowski, Joanne E. Porter, Michael Barbagallo, Val Prokopiv, Christopher Snell, Karen Missen
Summary: Physical inactivity is a global issue, with many adolescents and adults not meeting daily exercise recommendations. This systematic literature review examined the efficacy of workplace physical activity programs and identified barriers and enablers. Eighteen papers met the eligibility criteria, and four themes emerged from the data: Benefits to physical and mental health, barriers to workplace physical activity, and enablers. The findings provide recommendations for organizations aiming to improve the health of their employees, highlighting the positive outcomes of workplace physical activity programs.
Article
Social Sciences, Interdisciplinary
Elizabeth M. Miller, Joanne E. Porter, Michael S. Barbagallo
Summary: Qualitative case study research is often overlooked due to conflicting definitions and dense material. This article provides a step-by-step guide using Merriam and Stake's approaches with a palliative care setting as an example, aiming to help novice researchers understand and conduct case study research in a trustworthy and rigorous manner.
QUALITATIVE REPORT
(2023)
Review
Public, Environmental & Occupational Health
Michael S. Barbagallo, Sara Brito, Joanne E. Porter
Summary: Men's sheds are community-based organizations that provide a space for men to engage in hands-on activities and promote their health and wellbeing. This review examines the role of Australian men's sheds in improving the health and wellbeing of male participants. The review followed a systematic process and identified three key themes: health promotion and socialization, intergenerational mentoring, and Indigenous men's health. Men's sheds are ideal locations to deliver initiatives that positively impact male participants' health and wellbeing. Further research is needed to explore the implementation and evaluation of these initiatives in different communities.
HEALTH & SOCIAL CARE IN THE COMMUNITY
(2023)
Article
Nursing
Michael S. Barbagallo, Joanne E. Porter, Amany Abdelkader, Ainsley James
Summary: This study explored the learning experiences of undergraduate nursing students in an Anatomy and Physiology course through an open creative assessment task. The findings revealed that peer teaching and simplified learning methods brought the subject to life for students, while personalized learning catered to different learning styles. This qualitative exploratory study provides pedagogical insights into the use of art and creative approaches in teaching.
INTERNATIONAL JOURNAL OF NURSING EDUCATION SCHOLARSHIP
(2022)
Article
Computer Science, Interdisciplinary Applications
Amany Abdelkader, Michael S. Barbagallo
Summary: The COVID-19 pandemic caused disruptions to the delivery of Anatomy and Physiology courses to nursing students worldwide. This study evaluates nursing students' experiences and perceptions of transitioning to online study during the pandemic. Results show that face-to-face study mode was preferred, with a renewed appreciation for the blended study mode, while online study mode was the least preferred.
CIN-COMPUTERS INFORMATICS NURSING
(2022)